Next Article in Journal
Investigation of MnO2 and Ordered Mesoporous Carbon Composites as Electrocatalysts for Li-O2 Battery Applications
Next Article in Special Issue
Characterization of Nanoparticle Dispersion in Red Blood Cell Suspension by the Lattice Boltzmann-Immersed Boundary Method
Previous Article in Journal
Synthesis of Nickel Nanowires with Tunable Characteristics
Previous Article in Special Issue
Determination of Cd2+ and Pb2+ Based on Mesoporous Carbon Nitride/Self-Doped Polyaniline Nanofibers and Square Wave Anodic Stripping Voltammetry
Article Menu

Export Article

Open AccessCommunication
Nanomaterials 2016, 6(1), 20; doi:10.3390/nano6010020

A Graphene Oxide-Based Fluorescent Platform for Probing of Phosphatase Activity

College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Ming Su
Received: 16 November 2015 / Revised: 23 December 2015 / Accepted: 12 January 2016 / Published: 18 January 2016
(This article belongs to the Special Issue Nanomaterials for Biosensing Applications)
View Full-Text   |   Download PDF [1396 KB, uploaded 18 January 2016]   |  

Abstract

We presented a strategy for fabricating graphene oxide (GO)-based fluorescent biosensors to monitor the change of phosphorylation state and detect phosphatase activity. By regulating the interaction between the negatively charged phosphate group and the positively charged amino residue, we found that GO showed different quenching efficiency toward the phosphorylated and dephosphorylated dye-labeled peptides. To demonstrate the application of our method, alkaline phosphatase (ALP) was tested as a model enzyme with phosphorylated fluorescein isothiocyanate (FITC)-labeled short peptide FITC–Gly–Gly–Gly–Tyr(PO32−)–Arg as the probe. When the negatively charged phosphate group in the Tyr residue was removed from the peptide substrate by enzymatic hydrolysis, the resulting FITC–Gly–Gly–Gly–Tyr–Arg was readily adsorbed onto the GO surface through electrostatic interaction. As a result, fluorescence quenching was observed. Furthermore, the method was applied for the screening of phosphatase inhibitors. View Full-Text
Keywords: graphene oxide; fluorescent biosensors; phosphatase; peptide substrate; fluorescence quenching graphene oxide; fluorescent biosensors; phosphatase; peptide substrate; fluorescence quenching
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Sun, T.; Xia, N.; Liu, L. A Graphene Oxide-Based Fluorescent Platform for Probing of Phosphatase Activity. Nanomaterials 2016, 6, 20.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Nanomaterials EISSN 2079-4991 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top