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Nanomaterials 2016, 6(1), 20;

A Graphene Oxide-Based Fluorescent Platform for Probing of Phosphatase Activity

College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China
Authors to whom correspondence should be addressed.
Academic Editor: Ming Su
Received: 16 November 2015 / Revised: 23 December 2015 / Accepted: 12 January 2016 / Published: 18 January 2016
(This article belongs to the Special Issue Nanomaterials for Biosensing Applications)
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We presented a strategy for fabricating graphene oxide (GO)-based fluorescent biosensors to monitor the change of phosphorylation state and detect phosphatase activity. By regulating the interaction between the negatively charged phosphate group and the positively charged amino residue, we found that GO showed different quenching efficiency toward the phosphorylated and dephosphorylated dye-labeled peptides. To demonstrate the application of our method, alkaline phosphatase (ALP) was tested as a model enzyme with phosphorylated fluorescein isothiocyanate (FITC)-labeled short peptide FITC–Gly–Gly–Gly–Tyr(PO32−)–Arg as the probe. When the negatively charged phosphate group in the Tyr residue was removed from the peptide substrate by enzymatic hydrolysis, the resulting FITC–Gly–Gly–Gly–Tyr–Arg was readily adsorbed onto the GO surface through electrostatic interaction. As a result, fluorescence quenching was observed. Furthermore, the method was applied for the screening of phosphatase inhibitors. View Full-Text
Keywords: graphene oxide; fluorescent biosensors; phosphatase; peptide substrate; fluorescence quenching graphene oxide; fluorescent biosensors; phosphatase; peptide substrate; fluorescence quenching

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Sun, T.; Xia, N.; Liu, L. A Graphene Oxide-Based Fluorescent Platform for Probing of Phosphatase Activity. Nanomaterials 2016, 6, 20.

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