Next Article in Journal / Special Issue
Morphological, Chemical Surface, and Diffusive Transport Characterizations of a Nanoporous Alumina Membrane
Previous Article in Journal
Upconverting NIR Photons for Bioimaging
Previous Article in Special Issue
Recent Advance on Mesoporous Silica Nanoparticles-Based Controlled Release System: Intelligent Switches Open up New Horizon
Article Menu

Export Article

Open AccessArticle
Nanomaterials 2015, 5(4), 2169-2191; doi:10.3390/nano5042169

Utilization of Enzyme-Immobilized Mesoporous Silica Nanocontainers (IBN-4) in Prodrug-Activated Cancer Theranostics

1
Department of Life Science and Institute of Biotechnology, National Dong Hwa University, Hualien-974, Taiwan
2
Department of Chemistry, Kakatiya University, Telangana State-506009, India
3
Department of Chemistry, Tamkang University, New Taipei City 251, Taiwan
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editors: Jordi Sort and Eva Pellicer
Received: 25 September 2015 / Accepted: 26 November 2015 / Published: 4 December 2015
(This article belongs to the Special Issue Frontiers in Mesoporous Nanomaterials)
View Full-Text   |   Download PDF [2586 KB, uploaded 4 December 2015]   |  

Abstract

To develop a carrier for use in enzyme prodrug therapy, Horseradish peroxidase (HRP) was immobilized onto mesoporous silica nanoparticles (IBN-4: Institute of Bioengineering and Nanotechnology), where the nanoparticle surfaces were functionalized with 3-aminopropyltrimethoxysilane and further conjugated with glutaraldehyde. Consequently, the enzymes could be stabilized in nanochannels through the formation of covalent imine bonds. This strategy was used to protect HRP from immune exclusion, degradation and denaturation under biological conditions. Furthermore, immobilization of HRP in the nanochannels of IBN-4 nanomaterials exhibited good functional stability upon repetitive use and long-term storage (60 days) at 4 °C. The generation of functionalized and HRP-immobilized nanomaterials was further verified using various characterization techniques. The possibility of using HRP-encapsulated IBN-4 materials in prodrug cancer therapy was also demonstrated by evaluating their ability to convert a prodrug (indole-3- acetic acid (IAA)) into cytotoxic radicals, which triggered tumor cell apoptosis in human colon carcinoma (HT-29 cell line) cells. A lactate dehydrogenase (LDH) assay revealed that cells could be exposed to the IBN-4 nanocomposites without damaging their membranes, confirming apoptotic cell death. In summary, we demonstrated the potential of utilizing large porous mesoporous silica nanomaterials (IBN-4) as enzyme carriers for prodrug therapy. View Full-Text
Keywords: mesoporous silica nanoparticles; enzyme immobilization; Institute of Bioengineering and Nanotechnology (IBN-4); anti-cancer; prodrug therapy mesoporous silica nanoparticles; enzyme immobilization; Institute of Bioengineering and Nanotechnology (IBN-4); anti-cancer; prodrug therapy
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Hung, B.-Y.; Kuthati, Y.; Kankala, R.K.; Kankala, S.; Deng, J.-P.; Liu, C.-L.; Lee, C.-H. Utilization of Enzyme-Immobilized Mesoporous Silica Nanocontainers (IBN-4) in Prodrug-Activated Cancer Theranostics. Nanomaterials 2015, 5, 2169-2191.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Nanomaterials EISSN 2079-4991 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top