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Nanomaterials 2015, 5(3), 1211-1222;

Facile Attachment of TAT Peptide on Gold Monolayer Protected Clusters: Synthesis and Characterization

Advanced Materials Division, DST/Mintek Nanotechnology Innovation Centre, Mintek, Private Bag X3015, Randburg 2125, South Africa
Department of Chemistry, University of Zululand, Private Bag X1001, KwaDlangezwa 3886, South Africa
Present Address: Sasol (Pty) Ltd., P.O. Box 1, Sasolburg 1947, South Africa
Present Address: CSIR Materials Science and Manufacturing, P.O. Box 395, Pretoria 0001, South Africa
Authors to whom correspondence should be addressed.
Academic Editor: Thomas Nann
Received: 20 April 2015 / Revised: 20 April 2015 / Accepted: 29 May 2015 / Published: 21 July 2015
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High affinity thiolate-based polymeric capping ligands are known to impart stability onto nanosized gold nanoparticles. Due to the stable gold-sulfur bond, the ligand forms a protective layer around the gold core and subsequently controls the physicochemical properties of the resultant nanogold mononuclear protected clusters (AuMPCs). The choice of ligands to use as surfactants for AuMPCs largely depends on the desired degree of hydrophilicity and biocompatibility of the MPCs, normally dictated by the intended application. Subsequent surface modification of AuMPCs allows further conjugation of additional biomolecules yielding bilayer or multilayered clusters suitable for bioanalytical applications ranging from targeted drug delivery to diagnostics. In this study, we discuss our recent laboratory findings on a simple route for the introduction of Trans-Activator of Transcription (TAT) peptide onto the surface of biotin-derivatised gold MPCs via the biotin-strepavidin interaction. By changing the surface loading of biotin, controlled amounts of TAT could be attached. This bioconjugate system is very attractive as a carrier in intercellular delivery of various delivery cargoes such as antibodies, proteins and oligonucleotides. View Full-Text
Keywords: nanoparticles; MPCs; gold; TAT; peptide; biotin nanoparticles; MPCs; gold; TAT; peptide; biotin

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Sosibo, N.M.; Keter, F.K.; Skepu, A.; Tshikhudo, R.T.; Revaprasadu, N. Facile Attachment of TAT Peptide on Gold Monolayer Protected Clusters: Synthesis and Characterization. Nanomaterials 2015, 5, 1211-1222.

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