Next Article in Journal
Meso-Structuring of SiCN Ceramics by Polystyrene Templates
Next Article in Special Issue
Hybrid Mesoporous Silicas and Microporous POSS-Based Frameworks Incorporating Evaporation-Induced Self-Assembly
Previous Article in Journal
Separator Membrane from Crosslinked Poly(Vinyl Alcohol) and Poly(Methyl Vinyl Ether-alt-Maleic Anhydride)
Article Menu

Export Article

Open AccessCommunication
Nanomaterials 2015, 5(2), 415-424; doi:10.3390/nano5020415

Fabrication and Cell Responsive Behavior of Macroporous PLLA/Gelatin Composite Scaffold with Hierarchical Micro-Nano Pore Structure

1
State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China
2
Medicine Department, Dalian University, Dalian 116622, China
3
Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, 639798 Singapore, Singapore
4
Department of Obstetrics and Gynecology, First Affiliated Hospital, Dalian Medical University, Dalian 116011, China
These authors contribute equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editors: Jordi Sort and Eva Pellicer
Received: 20 November 2014 / Revised: 16 March 2015 / Accepted: 17 March 2015 / Published: 25 March 2015
(This article belongs to the Special Issue Frontiers in Mesoporous Nanomaterials)
View Full-Text   |   Download PDF [485 KB, uploaded 25 March 2015]   |  

Abstract

Scaffolds providing a 3D environment which can effectively promote the adhesion, proliferation and differentiation of cells are crucial to tissue regeneration. In this study, the poly-l-lactic acid (PLLA) scaffold with hierarchical pore structural was fabricated via two-step thermally induced phase separation (TIPS). To mimic both physical architecture and chemical composite of natural bone extracellular matrix (ECM), gelatin fibers were introduced into the pores of PLLA scaffolds and formed 3D network structure via TIPS. Human adipose tissue-derived stem cells (ADSCs) were harvested and seeded into PLLA/gel hybrid scaffolds and cultured in vitro for biocompatibility assay. The surface morphology, porosity and compressive modulus of scaffolds were characterized by scanning electron microscopy (SEM), density analysis and compression test respectively. The results showed that hybrid scaffolds had high porosity (91.62%), a good compressive modulus (2.79 ± 0.20 MPa), nanometer fibers (diameter around 186.39~354.30 nm) and different grades of pore size from 7.41 ± 2.64 nm to 387.94 ± 102.48 nm. The scaffolds with mild hydrolysis by NaOH were modified by 1-ethyl-3-(3-dimethyl ami-nopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS). Gelatin was performed onto PLLA scaffold via TIPS aiming at enhancement cell-material interaction. In comparison with PLLA scaffold, the PLLA/gel scaffold had better biological performance and the mechanical properties because the gelatin fibers homogeneously distributed in each pore of PLLA scaffold and formed 3D network structure. View Full-Text
Keywords: poly-l-lactic acid (PLLA); gelatin; thermally induced phase separation; human adipose derived stem cells; bone tissue engineering poly-l-lactic acid (PLLA); gelatin; thermally induced phase separation; human adipose derived stem cells; bone tissue engineering
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Song, K.; Ji, L.; Zhang, J.; Wang, H.; Jiao, Z.; Mayasari, L.; Fu, X.; Liu, T. Fabrication and Cell Responsive Behavior of Macroporous PLLA/Gelatin Composite Scaffold with Hierarchical Micro-Nano Pore Structure. Nanomaterials 2015, 5, 415-424.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Nanomaterials EISSN 2079-4991 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top