Nanomaterials 2013, 3(2), 221-228; doi:10.3390/nano3020221
Communication

Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide

email and * email
Received: 18 March 2013; in revised form: 27 March 2013 / Accepted: 28 March 2013 / Published: 4 April 2013
(This article belongs to the Special Issue Nanomaterials in Sensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Separation of short single- and double-stranded DNA typically requires gel electrophoresis followed by DNA extraction, which is a time consuming process. Graphene oxide adsorbs single-stranded DNA more quickly than double-stranded ones, allowing for selective removal of the former with a simple mixing and centrifugation operation. The effect of DNA length and salt on adsorption selectivity has been characterized and its application in DNA melting curve measurement has been demonstrated.
Keywords: graphene; DNA; adsorption; fluorescence; separation; gel electrophoresis
PDF Full-text Download PDF Full-Text [196 KB, uploaded 4 April 2013 09:51 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Huang, P.-J.J.; Liu, J. Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide. Nanomaterials 2013, 3, 221-228.

AMA Style

Huang P-JJ, Liu J. Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide. Nanomaterials. 2013; 3(2):221-228.

Chicago/Turabian Style

Huang, Po-Jung J.; Liu, Juewen. 2013. "Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide." Nanomaterials 3, no. 2: 221-228.

Nanomaterials EISSN 2079-4991 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert