Nanomaterials 2013, 3(2), 221-228; doi:10.3390/nano3020221
Communication

Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide

Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada
* Author to whom correspondence should be addressed.
Received: 18 March 2013; in revised form: 27 March 2013 / Accepted: 28 March 2013 / Published: 4 April 2013
(This article belongs to the Special Issue Nanomaterials in Sensors)
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Abstract: Separation of short single- and double-stranded DNA typically requires gel electrophoresis followed by DNA extraction, which is a time consuming process. Graphene oxide adsorbs single-stranded DNA more quickly than double-stranded ones, allowing for selective removal of the former with a simple mixing and centrifugation operation. The effect of DNA length and salt on adsorption selectivity has been characterized and its application in DNA melting curve measurement has been demonstrated.
Keywords: graphene; DNA; adsorption; fluorescence; separation; gel electrophoresis

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MDPI and ACS Style

Huang, P.-J.J.; Liu, J. Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide. Nanomaterials 2013, 3, 221-228.

AMA Style

Huang P-JJ, Liu J. Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide. Nanomaterials. 2013; 3(2):221-228.

Chicago/Turabian Style

Huang, Po-Jung J.; Liu, Juewen. 2013. "Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide." Nanomaterials 3, no. 2: 221-228.

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