Next Article in Journal
Mn2+-ZnSe/ZnS@SiO2 Nanoparticles for Turn-on Luminescence Thiol Detection
Next Article in Special Issue
Dental Composite Formulation Design with Bioactivity on Protein Adsorption Combined with Crack-Healing Capability
Previous Article in Journal / Special Issue
Collective Migration of Lens Epithelial Cell Induced by Differential Microscale Groove Patterns
Article Menu

Export Article

Open AccessFeature PaperArticle
J. Funct. Biomater. 2017, 8(3), 35; doi:10.3390/jfb8030035

Correlation and Comparison of Cortical and Hippocampal Neural Progenitor Morphology and Differentiation through the Use of Micro- and Nano-Topographies

1
Mechanobiology Institute, National University of Singapore, T-Lab, #05-01, 5A Engineering Drive 1, Singapore 117411
2
Department of Biomedical Engineering, National University of Singapore, 4 Engineering Drive 3, Engineering Block 4, #04-08, Singapore 117583
3
Institut für Biophysik, Universität Bremen, Otto-Hahn-Allee 1, Bremen 28359, Germany
4
Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, NUHS Tower Block, Level 8, 1E Kent Ridge Road, Singapore 119228
5
Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, ON N2L 3G1, Canada
*
Author to whom correspondence should be addressed.
Received: 1 July 2017 / Revised: 3 August 2017 / Accepted: 7 August 2017 / Published: 12 August 2017
(This article belongs to the Special Issue Journal of Functional Biomaterials: Feature Papers 2016)
View Full-Text   |   Download PDF [2101 KB, uploaded 12 August 2017]   |  

Abstract

Neuronal morphology and differentiation have been extensively studied on topography. The differentiation potential of neural progenitors has been shown to be influenced by brain region, developmental stage, and time in culture. However, the neurogenecity and morphology of different neural progenitors in response to topography have not been quantitatively compared. In this study, the correlation between the morphology and differentiation of hippocampal and cortical neural progenitor cells was explored. The morphology of differentiated neural progenitors was quantified on an array of topographies. In spite of topographical contact guidance, cell morphology was observed to be under the influence of regional priming, even after differentiation. This influence of regional priming was further reflected in the correlations between the morphological properties and the differentiation efficiency of the cells. For example, neuronal differentiation efficiency of cortical neural progenitors showed a negative correlation with the number of neurites per neuron, but hippocampal neural progenitors showed a positive correlation. Correlations of morphological parameters and differentiation were further enhanced on gratings, which are known to promote neuronal differentiation. Thus, the neurogenecity and morphology of neural progenitors is highly responsive to certain topographies and is committed early on in development. View Full-Text
Keywords: neuronal differentiation; topography; cell morphology; neural progenitor cells; neurons; astrocytes; gratings; polydimethlysiloxane; correlation analysis; quantitative comparison neuronal differentiation; topography; cell morphology; neural progenitor cells; neurons; astrocytes; gratings; polydimethlysiloxane; correlation analysis; quantitative comparison
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Sathe, S.; Chan, X.Q.; Jin, J.; Bernitt, E.; Döbereiner, H.-G.; Yim, E.K. Correlation and Comparison of Cortical and Hippocampal Neural Progenitor Morphology and Differentiation through the Use of Micro- and Nano-Topographies. J. Funct. Biomater. 2017, 8, 35.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
J. Funct. Biomater. EISSN 2079-4983 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top