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J. Funct. Biomater. 2015, 6(1), 1-13; doi:10.3390/jfb6010001

Caffeic Acid-PLGA Conjugate to Design Protein Drug Delivery Systems Stable to Irradiation

1
Università degli Studi di Milano, Department of Pharmaceutical Sciences—via Giuseppe Colombo, 71-20133 Milano, Italy
2
Università degli Studi della Calabria, Department of Pharmacy, Health and Nutritional Sciences, 87036 Arcavacata di Rende, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Pankaj Vadgama
Received: 25 September 2014 / Accepted: 24 December 2014 / Published: 5 January 2015
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Abstract

This work reports the feasibility of caffeic acid grafted PLGA (g-CA-PLGA) to design biodegradable sterile microspheres for the delivery of proteins. Ovalbumin (OVA) was selected as model compound because of its sensitiveness of γ-radiation. The adopted grafting procedure allowed us to obtain a material with good free radical scavenging properties, without a significant modification of Mw and Tg of the starting PLGA (Mw PLGA = 26.3 ± 1.3 kDa vs. Mw g-CA-PLGA = 22.8 ± 0.7 kDa; Tg PLGA = 47.7 ± 0.8 °C vs. Tg g-CA-PLGA = 47.4 ± 0.2 °C). By using a W1/O/W2 technique, g-CA-PLGA improved the encapsulation efficiency (EE), suggesting that the presence of caffeic residues improved the compatibility between components (EEPLGA = 35.0% ± 0.7% vs. EEg-CA-PLGA = 95.6% ± 2.7%). Microspheres particle size distribution ranged from 15 to 50 µm. The zeta-potential values of placebo and loaded microspheres were −25 mV and −15 mV, respectively. The irradiation of g-CA-PLGA at the dose of 25 kGy caused a less than 1% variation of Mw and the degradation patterns of the non-irradiated and irradiated microspheres were superimposable. The OVA content in g-CA-PLGA microspheres decreased to a lower extent with respect to PLGA microspheres. These results suggest that g-CA-PLGA is a promising biodegradable material to microencapsulate biological drugs. View Full-Text
Keywords: anti-oxidant; microencapsulation; drug release; caffeic acid; grafting; γ-irradiation; microspheres; ovalbumin; poly(lactide-co-glycolide); sterilization anti-oxidant; microencapsulation; drug release; caffeic acid; grafting; γ-irradiation; microspheres; ovalbumin; poly(lactide-co-glycolide); sterilization
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Selmin, F.; Puoci, F.; Parisi, O.I.; Franzé, S.; Musazzi, U.M.; Cilurzo, F. Caffeic Acid-PLGA Conjugate to Design Protein Drug Delivery Systems Stable to Irradiation. J. Funct. Biomater. 2015, 6, 1-13.

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