Caffeic Acid-PLGA Conjugate to Design Protein Drug Delivery Systems Stable to Irradiation
AbstractThis work reports the feasibility of caffeic acid grafted PLGA (g-CA-PLGA) to design biodegradable sterile microspheres for the delivery of proteins. Ovalbumin (OVA) was selected as model compound because of its sensitiveness of γ-radiation. The adopted grafting procedure allowed us to obtain a material with good free radical scavenging properties, without a significant modification of Mw and Tg of the starting PLGA (Mw PLGA = 26.3 ± 1.3 kDa vs. Mw g-CA-PLGA = 22.8 ± 0.7 kDa; Tg PLGA = 47.7 ± 0.8 °C vs. Tg g-CA-PLGA = 47.4 ± 0.2 °C). By using a W1/O/W2 technique, g-CA-PLGA improved the encapsulation efficiency (EE), suggesting that the presence of caffeic residues improved the compatibility between components (EEPLGA = 35.0% ± 0.7% vs. EEg-CA-PLGA = 95.6% ± 2.7%). Microspheres particle size distribution ranged from 15 to 50 µm. The zeta-potential values of placebo and loaded microspheres were −25 mV and −15 mV, respectively. The irradiation of g-CA-PLGA at the dose of 25 kGy caused a less than 1% variation of Mw and the degradation patterns of the non-irradiated and irradiated microspheres were superimposable. The OVA content in g-CA-PLGA microspheres decreased to a lower extent with respect to PLGA microspheres. These results suggest that g-CA-PLGA is a promising biodegradable material to microencapsulate biological drugs. View Full-Text
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Selmin, F.; Puoci, F.; Parisi, O.I.; Franzé, S.; Musazzi, U.M.; Cilurzo, F. Caffeic Acid-PLGA Conjugate to Design Protein Drug Delivery Systems Stable to Irradiation. J. Funct. Biomater. 2015, 6, 1-13.
Selmin F, Puoci F, Parisi OI, Franzé S, Musazzi UM, Cilurzo F. Caffeic Acid-PLGA Conjugate to Design Protein Drug Delivery Systems Stable to Irradiation. Journal of Functional Biomaterials. 2015; 6(1):1-13.Chicago/Turabian Style
Selmin, Francesca; Puoci, Francesco; Parisi, Ortensia I.; Franzé, Silvia; Musazzi, Umberto M.; Cilurzo, Francesco. 2015. "Caffeic Acid-PLGA Conjugate to Design Protein Drug Delivery Systems Stable to Irradiation." J. Funct. Biomater. 6, no. 1: 1-13.