J. Funct. Biomater. 2013, 4(2), 74-88; doi:10.3390/jfb4020074
Article

Incorporation of Exogenous RGD Peptide and Inter-Species Blending as Strategies for Enhancing Human Corneal Limbal Epithelial Cell Growth on Bombyx mori Silk Fibroin Membranes

Received: 22 March 2013; in revised form: 6 May 2013 / Accepted: 6 May 2013 / Published: 17 May 2013
(This article belongs to the Special Issue Advances in Ophthalmic Biomaterials)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: While fibroin isolated from the cocoons of domesticated silkworm Bombyx mori supports growth of human corneal limbal epithelial (HLE) cells, the mechanism of cell attachment remains unclear. In the present study we sought to enhance the attachment of HLE cells to membranes of Bombyx mori silk fibroin (BMSF) through surface functionalization with an arginine-glycine-aspartic acid (RGD)-containing peptide. Moreover, we have examined the response of HLE cells to BMSF when blended with the fibroin produced by a wild silkworm, Antheraea pernyi, which is known to contain RGD sequences within its primary structure. A procedure to isolate A. pernyi silk fibroin (APSF) from the cocoons was established, and blends of the two fibroins were prepared at five different BMSF/APSF ratios. In another experiment, BMSF surface was modified by binding chemically the GRGDSPC peptide using a water-soluble carbodiimide. Primary HLE were grown in the absence of serum on membranes made of BMSF, APSF, and their blends, as well as on RGD-modified BMSF. There was no statistically significant enhancing effect on the cell attachment due to the RGD presence. This suggests that the adhesion through RGD ligands may have a complex mechanism, and the investigated strategies are of limited value unless the factors contributing to this mechanism become better known.
Keywords: silk fibroin; corneal limbal epithelial cells; RGD peptide; fibroin blends; cell adhesion
PDF Full-text Download PDF Full-Text [376 KB, uploaded 17 May 2013 15:49 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Bray, L.J.; Suzuki, S.; Harkin, D.G.; Chirila, T.V. Incorporation of Exogenous RGD Peptide and Inter-Species Blending as Strategies for Enhancing Human Corneal Limbal Epithelial Cell Growth on Bombyx mori Silk Fibroin Membranes. J. Funct. Biomater. 2013, 4, 74-88.

AMA Style

Bray LJ, Suzuki S, Harkin DG, Chirila TV. Incorporation of Exogenous RGD Peptide and Inter-Species Blending as Strategies for Enhancing Human Corneal Limbal Epithelial Cell Growth on Bombyx mori Silk Fibroin Membranes. Journal of Functional Biomaterials. 2013; 4(2):74-88.

Chicago/Turabian Style

Bray, Laura J.; Suzuki, Shuko; Harkin, Damien G.; Chirila, Traian V. 2013. "Incorporation of Exogenous RGD Peptide and Inter-Species Blending as Strategies for Enhancing Human Corneal Limbal Epithelial Cell Growth on Bombyx mori Silk Fibroin Membranes." J. Funct. Biomater. 4, no. 2: 74-88.

J. Funct. Biomater. EISSN 2079-4983 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert