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J. Mar. Sci. Eng. 2017, 5(2), 22; doi:10.3390/jmse5020022

An Alternative Method to Niskin Sampling for Molecular Analysis of the Marine Environment

1
School of Marine Science and Engineering, Plymouth University, Drake Circus, Plymouth PL4 8AA, UK
2
Interface Analysis Center (IAC), HH Wills Physics Laboratory, Tyndall Ave, Bristol BS8 1TL, UK
3
Plymouth Marine Laboratory (PML), Prospect Place, Plymouth PL1 3DH, UK
4
Sir Alister Hardy Foundation for Ocean Science (SAHFOS), Citadel Hill, Plymouth PL1 2PB, UK
*
Author to whom correspondence should be addressed.
Academic Editor: Magnus Wahlberg
Received: 9 March 2017 / Revised: 22 May 2017 / Accepted: 31 May 2017 / Published: 2 June 2017
(This article belongs to the Special Issue Emerging Biomolecular Technologies in Marine Science)
View Full-Text   |   Download PDF [1086 KB, uploaded 23 June 2017]   |  

Abstract

The development of low-cost, open-source Remotely Operated Vehicle (ROV) systems has provided almost unrestricted access for researchers looking to monitor the marine environment in ever greater resolution. Sampling microbial communities from the marine environment, however, still usually relies on Niskin-bottle sampling (ROV or Conductivity-Temperature-Depth sampler (CTD) based), a method which introduces an inaccuracy and variability that is incompatible with metatranscriptomic analysis, for example. Here, we describe a versatile, easily-replicated platform which achieves in situ mRNA preservation, via the addition of RNAlater to filtered microbial cells, to enhance ROV or CTD functionality. View Full-Text
Keywords: remotely operated vehicle; metatranscriptomics; Niskin remotely operated vehicle; metatranscriptomics; Niskin
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Teague, J.; Scott, T.B.; Sharma, S.; Graham, G.; Allen, M.J. An Alternative Method to Niskin Sampling for Molecular Analysis of the Marine Environment. J. Mar. Sci. Eng. 2017, 5, 22.

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