An Alternative Method to Niskin Sampling for Molecular Analysis of the Marine Environment
AbstractThe development of low-cost, open-source Remotely Operated Vehicle (ROV) systems has provided almost unrestricted access for researchers looking to monitor the marine environment in ever greater resolution. Sampling microbial communities from the marine environment, however, still usually relies on Niskin-bottle sampling (ROV or Conductivity-Temperature-Depth sampler (CTD) based), a method which introduces an inaccuracy and variability that is incompatible with metatranscriptomic analysis, for example. Here, we describe a versatile, easily-replicated platform which achieves in situ mRNA preservation, via the addition of RNAlater to filtered microbial cells, to enhance ROV or CTD functionality. View Full-Text
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Teague, J.; Scott, T.B.; Sharma, S.; Graham, G.; Allen, M.J. An Alternative Method to Niskin Sampling for Molecular Analysis of the Marine Environment. J. Mar. Sci. Eng. 2017, 5, 22.
Teague J, Scott TB, Sharma S, Graham G, Allen MJ. An Alternative Method to Niskin Sampling for Molecular Analysis of the Marine Environment. Journal of Marine Science and Engineering. 2017; 5(2):22.Chicago/Turabian Style
Teague, Jonathan; Scott, Thomas B.; Sharma, Sanjay; Graham, George; Allen, Michael J. 2017. "An Alternative Method to Niskin Sampling for Molecular Analysis of the Marine Environment." J. Mar. Sci. Eng. 5, no. 2: 22.
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