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J. Mar. Sci. Eng. 2016, 4(1), 22; doi:10.3390/jmse4010022

Methodological Studies on Estimates of Abundance and Diversity of Heterotrophic Flagellates from the Deep-Sea Floor

Department of General Ecology, Institute for Zoology, Biocenter Cologne, University of Cologne, Zülpicher Straße 47b, Cologne D-50674, Germany
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Academic Editor: Angelika Brandt
Received: 30 September 2015 / Revised: 8 February 2016 / Accepted: 15 February 2016 / Published: 3 March 2016
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Abstract

Extreme environmental conditions in the deep sea hamper access to protist communities. In combination with the potentially highly diverse species composition, it demands a wide range of methods to be applied at the same time to guarantee a high resolution of quantitative and qualitative studies of deep-sea heterotrophic flagellates (HF). Within this study, we present a possible combination of several culture-independent and culture-dependent methods available for investigating benthic deep-sea HF communities. Besides live-counting and fixation of HF, we refer to cultivation methods and molecular surveys using next generation sequencing. Laboratory ecological experiments under deep-sea conditions (high pressure, low temperature) could allow the approval of the potential deep-sea origin of sampled HF. The combination of different methods offers a unique possibility to receive detailed information on nanofaunal life in the deep sea. Specific fixation techniques to preserve samples directly at the sampling depth must be applied in further studies to reflect the real biodiversity of the largest habitat on earth. View Full-Text
Keywords: live-counting; liquid-aliquot; fixation; next generation sequencing; pressure; deep-sea nanofauna live-counting; liquid-aliquot; fixation; next generation sequencing; pressure; deep-sea nanofauna
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MDPI and ACS Style

Schoenle, A.; Jeuck, A.; Nitsche, F.; Venter, P.; Prausse, D.; Arndt, H. Methodological Studies on Estimates of Abundance and Diversity of Heterotrophic Flagellates from the Deep-Sea Floor. J. Mar. Sci. Eng. 2016, 4, 22.

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