Next Article in Journal
Hydrodynamic and Sediment Modelling within a Macro Tidal Estuary: Port Curtis Estuary, Australia
Next Article in Special Issue
The Dynamics, Ecological Variability and Estimated Carbon Stocks of Mangroves in Mahajamba Bay, Madagascar
Previous Article in Journal
Observations and Predictions of Wave Runup, Extreme Water Levels, and Medium-Term Dune Erosion during Storm Conditions
Previous Article in Special Issue
Artificial Crab Burrows Facilitate Desalting of Rooted Mangrove Sediment in a Microcosm Study
Article Menu

Export Article

Open AccessArticle
J. Mar. Sci. Eng. 2015, 3(3), 699-719; doi:10.3390/jmse3030699

Phylogenetic Diversity of Diazotrophs along an Experimental Nutrient Gradient in Mangrove Sediments

1
College of Marine Science, University of South Florida, 140 7th Avenue South, St. Petersburg, FL 33701, USA
2
University of Southern California, 3616 Trousdale Parkway, Los Angeles, CA 90089, USA
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Joseph M. Smoak
Received: 28 May 2015 / Accepted: 13 July 2015 / Published: 24 July 2015
(This article belongs to the Special Issue Biogeochemical Cycles in Mangrove Forests)
View Full-Text   |   Download PDF [1663 KB, uploaded 24 July 2015]   |  

Abstract

The diversity of diazotrophs was studied in the sediment of mangrove forests (Twin Cays, Belize) subjected to a long-term fertilization with nitrogen and phosphorus. Terminal Restriction Fragment Length Polymorphism (TRFLP) and cloning of PCR-amplified nifH genes were combined via in silico analysis to assign clones to TRFLP-nifH phylotypes, as well as to characterize the occurrence of phylotypes in response to environmental conditions. Results indicated that mangrove sediments from Belize harbor a unique diazotrophic community with a low metabolic diversity dominated by sulfate reducers. The variability of potential nitrogen-fixing sulfate reducers was explained by several environmental parameters, primarily by the abundance of dead roots in the sediments, and the concentration of H2S in the pore-waters. This study describes the complexity of microbial communities within the mangrove sediments with specific functional groups varying along environmental gradients. View Full-Text
Keywords: diazotrophs; mangrove sediments; phylogenetic analysis; generalized additive models diazotrophs; mangrove sediments; phylogenetic analysis; generalized additive models
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Romero, I.C.; Jacobson-Meyers, M.E.; Fuhrman, J.A.; Capone, D.G. Phylogenetic Diversity of Diazotrophs along an Experimental Nutrient Gradient in Mangrove Sediments. J. Mar. Sci. Eng. 2015, 3, 699-719.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
J. Mar. Sci. Eng. EISSN 2077-1312 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top