Next Article in Journal
Clarification of Orange Press Liquors by PVDF Hollow Fiber Membranes
Previous Article in Journal
Treatment of the Bleaching Effluent from Sulfite Pulp Production by Ceramic Membrane Filtration
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Membranes
Volume 6
Issue 1
10.3390/membranes6010008
membranes-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Lamina Associated Polypeptide 1 (LAP1) Interactome and Its Functional Features

by
Joana B. Serrano
,
Odete A. B. Da Cruz e Silva
and
Sandra Rebelo
*
Neuroscience and Signalling Laboratory, Department of Medical Sciences, Institute of Biomedicine—iBiMED, University of Aveiro, 3810-193 Aveiro, Portugal
*
Author to whom correspondence should be addressed.
Membranes 2016, 6(1), 8; https://doi.org/10.3390/membranes6010008
Submission received: 30 October 2015 / Revised: 5 January 2016 / Accepted: 6 January 2016 / Published: 15 January 2016
Browse Figures
Versions Notes

Abstract

:
Lamina-associated polypeptide 1 (LAP1) is a type II transmembrane protein of the inner nuclear membrane encoded by the human gene TOR1AIP1. LAP1 is involved in maintaining the nuclear envelope structure and appears be involved in the positioning of lamins and chromatin. To date, LAP1’s precise function has not been fully elucidated but analysis of its interacting proteins will permit unraveling putative associations to specific cellular pathways and cellular processes. By assessing public databases it was possible to identify the LAP1 interactome, and this was curated. In total, 41 interactions were identified. Several functionally relevant proteins, such as TRF2, TERF2IP, RIF1, ATM, MAD2L1 and MAD2L1BP were identified and these support the putative functions proposed for LAP1. Furthermore, by making use of the Ingenuity Pathways Analysis tool and submitting the LAP1 interactors, the top two canonical pathways were “Telomerase signalling” and “Telomere Extension by Telomerase” and the top functions “Cell Morphology”, “Cellular Assembly and Organization” and “DNA Replication, Recombination, and Repair”. Once again, putative LAP1 functions are reinforced but novel functions are emerging.

Graphical Abstract

1. Introduction

The eukaryotic nucleus is a complex organelle enclosed by a highly organized double membrane, the nuclear envelope (NE). The NE separates the nucleus from the cytoplasm and is essentially composed by the inner nuclear membrane (INM), the outer nuclear membrane (ONM), the nuclear pore complexes (NPCs) and nuclear lamina. The INM and ONM are separated by the perinuclear space of 40–50 nm of diameter and are crossed and therefore connected at the NPCs. The perinuclear space is continuous with the lumen of the rough endoplasmic reticulum (RER) and the ONM is continuous with the rough endoplasmic reticulum membrane that comprises the ribosomes. The lamins, which are type V intermediate filament proteins exclusively found in the nucleus and associated with INM proteins, can be found in the nuclear lamina. The NE and NE proteins have received more attention in the last few years and there is increasing evidence that the NE is responsible for integrating many cellular functions, including chromatin organization, signalling pathways, transcription regulation and cytoskeletal organization [1,2,3].
The nuclear membranes are considered an interconnected membrane system associated with the RER that comprises a specific group of proteins that are specifically enriched in the INM and ONM, but not in the RER. Of these, approximately 80 transmembrane proteins are concentrated in the INM [4] and a significantly lower number is concentrated in the ONM. Some of the INM proteins remain uncharacterized, but others were found to interact with lamins and/or chromatin. One of the first lamina associated proteins identified was lamina-associated polypeptide 1 (LAP1) [5] which is a type II transmembrane protein of the inner nuclear membrane, encoded by the human gene TOR1AIP1. In rats, three LAP1 isoforms were described and are derived by alternative RNA splicing, these are LAP1A, LAP1B and LAP1C with molecular weights of 75, 68 and 55 KDa, respectively [5,6]. In humans, the LAP1B isoform was previously identified by Kondo et al. [7] and a novel human isoform, the LAP1C, was recently identified. This new isoform is N-terminally truncated, with a molecular weight of approximately 55 KDa contrasting with the 68 KDa of the LAP1B [8]. The function of LAP1 remains poorly understood. However, several LAP1 binding partners have been identified as is the case with lamins (directly binding) and chromosomes (indirectly binding) [9]. Therefore it is assumed that LAP1 might be involved in the positioning of lamins and chromatin in close proximity to the NE, thereby contributing to the maintenance of NE structure [6,10]. Another important LAP1 binding protein is torsinA, which is the central protein in DYT1 dystonia [11]. A mutation of a single glutamic acid within torsinA (∆E-torsinA) is responsible for DYT1 dystonia, a dominantly inherited neurological and movement disorder characterized by prolonged involuntary twisting movements [12]. Interestingly, while the wild type torsinA is localized in both RER and the perinuclear space, the mutated torsinA (∆E-torsinA; pathogenic variant) preferentially concentrates in the perinuclear space [13,14]. Of note, torsinA variants that bind more efficiently to LAP1 do not hydrolyze ATP. Furthermore, LAP1 has been shown to bind torsinA and to activate its ATPase activity [15,16]. Recently, LAP1 was found to interact with another INM protein, namely emerin [17], the latter is associated with the X-linked Emery-Dreifuss muscular dystrophy [18]. The interaction of these two INM proteins is mediated via their nucleoplasmic domain, whereby emerin binds to LAP1 residues 1-330 [17]. We recently reported that the human LAP1B binds to protein phosphatase 1 (PP1) in the nucleoplasm domain and that it is dephosphorylated by this phosphatase [19]. Furthermore, five different LAP1 phosphorylated residues were identified: Ser143, Ser216, Thr221, Ser306 and Ser310. From these, it was possible to establish that only Ser306 and Ser310 are dephosphorylated by PP1 [8].
Recently, two mutations were identified in the TOR1AIP1 gene, that were directly associated with disease conditions. The first TOR1AIP1 mutation (“Turkish” mutation) reported, affects three members of a Turkish family with an autosomal recessive limb-girdle muscular dystrophy with joint contractures. It was predicted that this c.186delG mutation, truncates the 584 amino acid LAP1B protein to an apparently non-functional protein, only 83 amino acids long [20]. Consequently, expression of LAP1B was absent in the skeletal muscle fibers of these patients. The authors also showed, by ultrastructural examination of the muscle fibers, that patients had intact sarcomeric organization but clearly evident alterations of the nuclear envelope including nuclear fragmentation. Of note, this study places LAP1B as a pivotal protein associated with striatal muscle function and increases the number of genes associated with nuclear envelopathies. A second TOR1AIP1 mutation (“Maroccan” mutation) was reported in a boy, born from consanguineous healthy parents, who developed rapidly progressing dystonia, progressive cerebellar atrophy, and dilated cardiomyopathy. Upon whole exome sequencing a homozygous missense mutation in TOR1AIP1 was identified, resulting in a highly conserved glutamic acid change to alanine at amino acid 482 [21]. The in vivo studies performed by the authors indicated that the patients revealed reduced expression levels of LAP1 and its misallocation and aggregation in the endoplasmic reticulum [21]. Of note, both LAP1 human isoforms (LAP1B and C) are affected by this mutation. These two mutations strengthen functional association of LAP1 with DYT1 dystonia (in neurons) and muscular dystrophy (skeletal muscle) [22].
To date the precise LAP1 function is not fully elucidated. However a significant amount of work has been associated with the identification and functional characterization of LAP1 interactions, particularly with lamins, torsinA, emerin and PP1. The identification of additional LAP1 binding proteins and their association to specific cellular pathways and cellular functions are therefore crucial to understanding the precise physiological roles of LAP1. The work here described proposes novel LAP1 functions, through the bioinformatic analysis of the protein’s interactors.

2. Results and Discussion

In order to determine LAP1 interacting proteins the approach summarized in Figure 1 was used. Briefly, binary interactions with LAP1 were collected and manually curated to obtain the working dataset of LAP1 interactors of (step 2.1, Figure 1). Subsequent integration of the interactions among the listed interactors was performed with GeneMANIA, resulting in a more complete prospective network (step 2.2, Figure 1). GO and BiNGO enrichment analysis assisted in ascertaining the most prevalent biological processes and the cellular distribution of the interactors (step 2.3, Figure 1). Finally, by resorting to Ingenuity Pathway Analysis the physiological and functional relevance of the selected proteins were explored (step 2.4, Figure 1).
Membranes 06 00008 g001 1024
Figure 1. Methods overview. Schematic representation of the workflow; the steps necessary to integrate the Network of LAP1 integrators are depicted.
Figure 1. Methods overview. Schematic representation of the workflow; the steps necessary to integrate the Network of LAP1 integrators are depicted.
Membranes 06 00008 g001

2.1. LAP1 Interactor’s Network

Having curated information retrieved from the online databases, all the experimentally tested interactions (Table 1) were loaded into Cytoscape 3.2.1 [23] in order to manually build the network of LAP1 interactors (Figure 2). Of these interactions, 36 were verified in human (grey), one in mouse and rat (blue), one only in rat (green) and three between human LAP1 and viral proteins (pink for HIV-1, red for HRSVA and orange for HHV-4). All the white nodes represent proteins, while RNA is denoted as yellow nodes. Grey edges signify protein-protein interaction, while protein-RNA or RNA-RNA interactions are coloured in black (Figure 2).
Throughout the construction of this particular dataset one might consider the fact that every experimental method used in the study of protein-protein interactions has its drawbacks. In Table 1, the techniques included two-hybrid, affinity capture-MS/WB, co-fractionation and reconstituted complex. These techniques give completely different information but together they enable the identification of LAP1 putative biological functions. Two-hybrid and reconstructing complexes indicate direct protein-protein interactions, even if their biological relevance is questionable. Conversely, affinity capture-MS/WB and co-fractionation represent interactions that could be indirectly occurring in a complex. Consequently, one has to be critical when considering the interactions that are indicated in Table 1.
The methodology used for validation of the interaction and the number of times it was validated can strengthen the proposed interaction. More relevance should be given to the interactions proven multiple times through different techniques. For instance, the interaction between LAP1 and TorsinA has been validated in nine different publications by affinity capture-MS/WB and reconstituted complex methods. Additionally, the LAP1-TorsinA complex has been investigated several times [22,24] as well as the LAP1-Lamin A complex, which is associated to the maintenance of the nuclear envelope structure. Conversely, the Nuclear pore complex protein, Nup50, has been described with only one publication, using co-fractionation protocols but the biological function was not investigated. Considering these examples, there is stronger evidence for TorsinA and Lamin A as LAP1 interactors than for Nup50.
The bioinformatic analysis retrieved two RNA integrating interactions with LAP1. The first of these is ELAVL1, an RNA-binding protein that regulates the stability and translation of numerous mRNAs encoding proteins that respond to stress or proliferation [25]. ELAV-like protein 1 physically interacts with TOR1AIP1 mRNA (Figure 2), possibly promoting stability or influencing translation, in response to environmental changes [25]. The other RNA interaction described was with WHSC1, which is a histone methyltransferase (also known as MMSET) that has a sequence for ACA11 within an intron of its gene [26]. ACA11 is an orphan box H/ACA class small nuclear RNA (snoRNA) that is localized to the nucleoli and integrates a new snRNP (small nuclear ribonucleoprotein) complex that is involved in post-splicing intron complexes [26]. This snRNP is proposed to target other snoRNAs intermediates, in particular, snoRNAs hosted within ribosomal protein (RB) genes and to bind various nucleolar proteins associated with the regulation of RNA processing [26]. Functionally, ACA11 was found to suppress oxidative stress, confer resistance to chemotherapy and increase the proliferation in multiple myeloma cells. In the work of Chu and colleagues [26], LAP1 was found to bind this snoRNA, where both share binary interactions with HNRNPM, VIM, LMNA and LMNB1 (Figure 2) [26]. Given the absence of additional functional studies on ACA11, the meaning of its associations with LAP1 and its interactors remains unclear. However, the emerging hypothesis is that LAP1 might be associated with transcription regulation through the interaction with ACA11 and HNRNPM (Figure 2).
Moreover, a subset of viral proteins that integrate the LAP1 network, namely tat, 1C and LMP2 (Figure 2) was also identified. Tat is a nuclear regulatory protein crucial for HIV-1 replication that also coordinates HIV-1 provirus transcriptional regulation [27]. Alternatively, RNA viruses like HRSV have limited coding capacity, and their proteins often possess multiple functional domains with the ability to interact either with viral or cellular proteins [28]. This is the case for 1C, which binds various human proteins that are associated with transcriptional and cell cycle regulation [28]. Lastly, the Epstein-Barr virus LMP2B isoform co-localizes with LMP2A in perinuclear regions in transiently transfected cells [29], however LMP2 function in the NE remains unclear.
Due to the significant role of the NE as a cellular barrier, multiple viral organisms have developed the capability to modulate their permeability in order to infect the host cell (reviewed in [30]). Viruses can modulate NE permeability for different reasons. Some viruses disrupt NE in order to transport the viral genome into the nucleus for replication (HIV), while others cause NE disruption during nuclear egress of newly assembled capsids (HHV-4) (reviewed in [30]). In addition, many viruses modulate NE permeability either to destabilize compartmentalization of host proteins or to inhibit the nuclear transport of host antiviral response proteins (reviewed in [30]).
Table
Table 1. LAP1 interactors obtained from online databases after validating curated interactions via consulting respective publications.
Table 1. LAP1 interactors obtained from online databases after validating curated interactions via consulting respective publications.
GeneProteinUniprot Accession NumberSpeciesInteraction Detection MethodReferences
1CNon-structural protein 1P04544TOR1AIP1 Hs—1C HRSVAAffinity Capture-MS[28]
AIFM1Apoptosis-inducing factor 1, mitochondrialO95831Homo sapiensAffinity Capture-MS[31]
ATMSerine-protein kinase ATMQ13315Homo sapiensAffinity Capture-MS[32]
Atp1b4Protein ATP1B4Q99ME6Mus musculusTwo-hybrid[33]
Q9R193Rattus norvegicusAffinity Capture-WB
CA10Carbonic anhydrase-related protein 10Q9NS85Homo sapiensAffinity Capture-MS[34]
CANXCalnexinP35564Tor1aip1 Mm—CANX HsAffinity Capture-MS[35]
CCDC8Coiled-coil domain-containing protein 8Q9H0W5Homo sapiensAffinity Capture-MS[36]
EGFREpidermal growth factor receptorP00533Homo sapiensAffinity Capture-MS[37]
ELAVL1 *ELAV-like protein 1Q15717Homo sapiensAffinity Capture-RNA[25]
HNRNPMHeterogeneous nuclear ribonucleoprotein MP52272Homo sapiensCo-fractionation[38]
HPDL4-hydroxyphenylpyruvate dioxygenase-like proteinQ96IR7Homo sapiensCo-fractionation[38]
KLHL15Kelch-like protein 15Q96M94Homo sapiensAffinity Capture-MS[31]
LMNAPrelamin-A/CP48678Mus musculusAffinity Capture-MS[39]
P02545Homo sapiensTwo-hybrid[40]
Homo sapiensReconstituted Complex[9]
Homo sapiensProximity Label-MS[41]
Lmnb1Lamin-B1P70615Rattus norvegicusAffinity Capture-WB[42]
LMP2Latent membrane protein 2Q1HVJ2TOR1AIP1 Hs—LMP2 HHV-4Affinity Capture-MS[43]
LRRK2Leucine-rich repeat serine/threonine-protein kinase 2Q5S007Homo sapiensAffinity Capture-MS[44]
Mad2l1MAD2 mitotic arrest deficient-like 1Q9Z1B5TOR1AIP1 Hs—Mad2l1 MmAffinity Capture-MS[35]
MAD2L1BPMAD2 mitotic arrest deficient-like 1Q15013Homo sapiensAffinity Capture-MS[31]
MDM2E3 ubiquitin-protein ligase Mdm2Q00987Homo sapiensAffinity Capture-MS[45]
NDUFV1NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrialP49821Homo sapiensCo-fractionation[38]
NIT2Omega-amidase NIT2Q9NQR4Homo sapiensCo-fractionation[38]
NTRK1High affinity nerve growth factor receptorP04629Homo sapiensAffinity Capture-MS[46]
NUP50Nuclear pore complex protein Nup50Q9UKX7Homo sapiensCo-fractionation[38]
OXCT1Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrialP55809Homo sapiensCo-fractionation[38]
PPP1CASerine/threonine-protein phosphatase PP1-alpha catalytic subunitP62136Homo sapiensAffinity Capture-WB[47]
Two-hybrid[48]
PPP1CCSerine/threonine-protein phosphatase PP1-gamma catalytic subunitP36873Homo sapiensAffinity Capture-WB[47]
Two-hybrid[49]
PTCH1Protein patched homolog 1Q13635Homo sapiensAffinity Capture-MS[34]
RIF1Telomere-associated protein RIF1Q5UIP0Homo sapiensCo-fractionation[38]
SCARNA22 **Small Cajal body-specific RNA 22Gene ID: 677770Homo sapiensAffinity Capture-RNA[26]
S100A16Protein S100-A16Q96FQ6Homo sapiensCo-fractionation[38]
SEPT9Septin-9Q9UHD8Homo sapiensCo-fractionation[38]
TatProtein TatP04608TOR1AIP1 Hs—tat HIV-1Affinity Capture-MS[27]
TERF2Telomeric repeat-binding factor 2Q15554Homo sapiensTwo-hybrid[50]
TERF2IPTelomeric repeat-binding factor 2-interacting protein 1Q9NYB0Homo sapiensTwo-hybrid[50]
TOR1ATorsin-1AO14656Homo sapiensAffinity Capture-MS/WB[11,15,16,51,52]
Reconstituted Complex[52]
[16,52]
Affinity Capture-MS[53]
TOR1AIP1Lamina-associated polypeptide 1BQ5JTV8Tor1aip1 Mm—TOR1AIP1 HsAffinity Capture-MS[35]
TOR1BTorsin-1BO14657Homo sapiensAffinity Capture-WB[53]
TOR2ATorsin-2AQ5JU69Homo sapiensAffinity Capture-WB[53]
TOR3ATorsin-3AQ9H497Homo sapiensAffinity Capture-WB[53]
UBCPolyubiquitin-CP0CG48Homo sapiensAffinity Capture-MS[54,55,56,57,58,59]
VIMVimentinP08670Homo sapiensAffinity Capture-WB[60]
* signifies protein-RNA and ** represents RNA-RNA interactions. Hs, Homo sapiens; Mm, Mus musculus; HRSVA, Human respiratory syncytial virus A (strain A2); HHV-4, Human herpes virus 4 (Epstein-Barr virus (strain AG876)); HIV-1, Human Immunodeficiency Virus 1; MS, mass spectrometry; WB, western blot.
Membranes 06 00008 g002 1024
Figure 2. LAP1 interactome built using Cytoscape 3.2.1 [1]. Of these interactions, 36 were verified in human (grey); one in mouse and rat (blue); one only in rat (green); and three between human LAP1 and viral proteins (pink for HIV-1, red for HRSVA and orange for HHV-4). All the white nodes represent proteins, while RNA is denoted with yellow filling. Grey edges signify protein-protein interaction, while protein-RNA or RNA-RNA interactions are coloured with black edges. LAP1 is also able to form dimers, which is represented by a self-binding edge.
Figure 2. LAP1 interactome built using Cytoscape 3.2.1 [1]. Of these interactions, 36 were verified in human (grey); one in mouse and rat (blue); one only in rat (green); and three between human LAP1 and viral proteins (pink for HIV-1, red for HRSVA and orange for HHV-4). All the white nodes represent proteins, while RNA is denoted with yellow filling. Grey edges signify protein-protein interaction, while protein-RNA or RNA-RNA interactions are coloured with black edges. LAP1 is also able to form dimers, which is represented by a self-binding edge.
Membranes 06 00008 g002
The mammalian proteins’ interactions were analysed in the next section with the support of GeneMANIA.

2.2. Network Construction with GeneMANIA

In order to generate an extended protein-protein interaction network compiled by the set of proteins that were obtained previously, excluding RNA interactions and viral proteins, all the remaining binary interactions were imported to GeneMANIA [61]. Network mapping of LAP1 protein interactors was only possible by extrapolating the mouse and rat interactions to human. The version 3.4.0 of GeneMANIA [61] was installed into Cytoscape 3.2.1 [23] enabling network editing.
The set of gene IDs was introduced and the functional associative network was set to include only physical interactions between proteins. The output includes probable protein interactors in the interactome of LAP1, extending the network based on a guilt-by-association approach that derives predictions from a combination of potentially heterogeneous data sources. Thereby, the grey nodes are genes inferred by the GeneMANIA plugin. GeneMANIA extends the user’s list with genes that are functionally similar, or have shared properties with the initial query genes, and displays an interactive functional association network, illustrating the relationships among the genes and data sets [61]. The resulting network represents a prospective LAP1 interactome (Figure 3) that we propose to have functional relevance regarding its probable integrative role as a complex assembly or stabilization protein. For instance, LAP1 association with itself could be important to form dimers that provide a structural link between the nucleoskeleton (lamina) and cytoskeleton across the NE, as previously described for SUN domain proteins in the NE (reviewed in [62]).
Membranes 06 00008 g003 1024
Figure 3. LAP1 (TOR1AIP1) interacting network complemented by the GeneMANIA plugin in cytoscape. Missing binary interactions to TOR1AIP1 were added to the GeneMANIA output and are represented by the black edges, while the remaining are in pink. Black nodes signify the interactors inserted into the plugin and grey nodes denote GeneMANIA’s additional input. LAP1 is also able to form dimers, which is represented by a self-binding edge.
Figure 3. LAP1 (TOR1AIP1) interacting network complemented by the GeneMANIA plugin in cytoscape. Missing binary interactions to TOR1AIP1 were added to the GeneMANIA output and are represented by the black edges, while the remaining are in pink. Black nodes signify the interactors inserted into the plugin and grey nodes denote GeneMANIA’s additional input. LAP1 is also able to form dimers, which is represented by a self-binding edge.
Membranes 06 00008 g003
The association between MAD2L1 and MAD2L1BP occurs in the mitotic checkpoint complex (MCC) [63], and both proteins have been proposed to physically bind LAP1 and MAD1L1 [35] (Figure 3). The MCC inhibits the anaphase-promoting complex/cyclosome (APC/C), which is an E3 ubiquitin ligase that initiates chromosome segregation [64]. This inhibition regulates the spindle assembly checkpoint (SAC) which is responsible for delaying chromosome segregation until all sister chromatids achieve bipolar attachment to the mitotic spindle, ensuring genome stability [64]. The experimental evidence of LAP1’s direct interactions with MAD2L1 and MAD2L1BP reinforces the early hypothesis that LAP1 is a key regulator of mitosis [9,65], and strengthens the upcoming perspective that LAP1 might be functionally associated to the MCC.
Human LAP1B was previously described to be phosphorylated on Ser143, in a proline-directed manner similar to CDKs substrates [66], and on Ser164 within the ATM/ATR recognition sequence motifs [67]. Furthermore, rat LAP1B Ser142 (homologous to human LAP1B Ser143) was found to be phosphorylated by CDK1-cyclin B [68]. Indeed, LAP1 seems to be functionally associated with several signalling proteins that activate, inhibit and mediate signalling cascades. Among the most relevant regulators are protein kinases, in this case comprising EGFR, NTRK1, LRRK2 and ATM (Figure 3). DNA damage events, such as ultraviolet irradiation, result in the nuclear translocation of EGFR, in a ligand-independent pathway [69]. Similarly, LAP1 phosphorylation by ATM on S133, S145, S169 and S164 was associated to DNA damage, consolidating the hypothesis that LAP1 might be phosphorylated as a cellular response to genotoxic stress [32].
Alternatively, protein phosphatases PP1α (PPP1CA) and PP1γ (PPP1CC) were shown to regulate LAP1 by dephosphorylation, forming a complex that was validated in rat brain and cultured cells [19]. This reveals the role of LAP1 in complexes important in regulation, through phosphorylation. Moreover, the PP1 regulatory protein CCDC8 (alternatively termed PPP1R20) is a core component of the 3-M complex. This complex is required to regulate microtubule dynamics and genome integrity [36]. Correspondingly, CCDC8 associates with the mRNA splicing machinery, in particular to the HNRNP family, which is highly represented in the 3-M interactome [36]. The above-mentioned LAP1 mediated events illustrate, the potential dynamic role of LAP1, in the regulation of transcription and cytoskeleton mechanisms via interaction with CCDC8 (Figure 3).
LAP1 might communicate to the shelterin complex. The latter is an emerging protein complex with DNA remodelling activity that coordinates with DNA repair factors, to change the structure of the telomeric DNA, thereby protecting chromosome ends (reviewed in [70]). This complex is composed of TRF1 and TRF2 as double-stranded DNA binding proteins that recognize TTAGGG repeats [50]. TRF2 in particular is associated to TERF2IP which is then regulated by RIF1 (reviewed in [70]). RIF1 is required for checkpoint mediated arrest in response to DNA damage during the S-phase (the intra-S-phase checkpoint) [71]. This checkpoint can be activated by at least by two parallel pathways involving the ATM kinase [71]. LAP1 might have a role in assembly or stabilizing this particular subset of proteins of the shelterin complex, as it binds to TRF2, TERF2IP, RIF1 and ATM (Figure 3). Telomere associated functions have been previously described for various INM proteins such as LMNA, SUN1, LAP1 and BAF (reviewed in [72]).
The relevance of LAP1 interactions regarding biological processes and cellular localizations were analysed in the next section with the support of GO enrichment analysis [73] and BiNGO [74].

2.3. GO Term Enrichment Analysis

The online GO Consortium term enrichment service, supported by Panther (available at [75]) [73,76], was used to conduct a GO term enrichment analysis for the 38 LAP1 interactors described in Table 1. Viral connections (tat, 1C and LMP2) were excluded. The biological process and cellular component GO terms that were enriched among these target proteins were scored (Figure 4). All the IDs of the interactors were mapped with one exception, SCARNA22.
BiNGO [74] was alternatively used to build a directed acyclic graph (DAG) network that conveys visualization of the enriched terms organized in a tree-like structure, starting from more general terms at the root (for example, biological regulation) to the most specific at the leaves (for example, the regulation of mitotic cell cycle) [74]. Each term is coded by size and colour, so that larger nodes contain more genes and darker nodes are more significantly enriched. Insignificant intermediate terms are denoted as small white nodes [74] (Figures S1 and S2).
Upon examining the biological processes of the proteins found in the filtered list of interactions (Figure 4A and Figure S1), significant enrichment was observed in the processes of “regulation of response to DNA damage stimulus”, “nuclear membrane organization”, “nuclear envelope organization”, “cell cycle”, “chaperone mediated protein folding requiring cofactor”, “chaperone-mediated protein folding”, “nucleus organization”, “telomere maintenance”, “cellular component organization”, “telomere organization” and “cellular component disassembly involved in execution phase of apoptosis”. By analysing the cellular component that the protein interactors might integrate (Figure 4B and Figure S2), significant and relevant enrichment was found in the “nuclear envelope”, “chromosomal region”, “endoplasmic reticulum lumen”, “chromosome and telomeric region” and “lamin filament”.
This output reinforces the idea that LAP1 might function as a stabilizing element by forming multiple complexes as noted before. In particular, integrating a response to DNA damage through ATM activation and subsequently participating in telomere regulation, through the interaction with RIF1, TERF2IP and TRF2 [71]. Furthermore, there are strong evidences that LAP1 intervenes in mitotic regulation via association to the MCC complex proteins, as previously studied [9]. The vastly documented relationship with the Torsin family [77], resident in the endoplasmic reticulum, conveys the chaperone related annotations observed in the enrichment analysis. Finally, LAP1 collaboration with the NE cytoskeleton [9,77] outputs “nuclear membrane organization”, “nuclear envelope organization”, “cellular component organization” and “nucleus organization”, as some of the most prominent biological processes attributed to LAP1 interactors.
Membranes 06 00008 g004 1024
Figure 4. Significantly enriched Gene Ontology terms from LAP1 interaction network (A) Biological process; and (B) Cellular Component. Bonferroni corrected p-values (α = 0.05) were transformed by −log10.
Figure 4. Significantly enriched Gene Ontology terms from LAP1 interaction network (A) Biological process; and (B) Cellular Component. Bonferroni corrected p-values (α = 0.05) were transformed by −log10.
Membranes 06 00008 g004
Physiological and functional analysis of the pathways’ with which LAP1’s interactome integrates is presented in the next section, with the aid of the Ingenuity Pathway Analysis.

2.4. Ingenuity Pathways Analysis (IPA) Physiological and Functional Analysis

Gene identifications of the previously described interactors were imported into the Ingenuity Pathways Analysis (IPA) tool, once more extrapolating the mouse and rat interactions to human and excluding viral connections. Function classifications, signal pathways and interacting networks were constructed and analysed based on the underlying biological evidence from the IPA’s database. The core analysis performed in IPA retrieved seven distinctive functional sets: canonical pathways, upstream regulators, diseases and disorders, molecular and cellular functions, physiological system development and functions, associated network functions and toxicological lists that are depicted in Table 2, Table 3, Table 4, Table 5 and Table 6 and Figure 5, Figures S3–S6. Right-tailed Fisher’s exact test was used to calculate the significance of each biological function and/or disease assigned, which was reported if the adjusted p-value was below a significance threshold of 0.05.
Table
Table 2. Top Canonical Pathways for LAP1 interactors dataset.
Table 2. Top Canonical Pathways for LAP1 interactors dataset.
Namep-ValueOverlap %
Telomerase Signalling2.99 × 10−54.00%4/99
Telomere Extension by Telomerase3.28 × 10−413.30%2/15
HER-2 Signalling in Breast Cancer3.47 × 10−43.90%3/76
Glioma Signalling6.68 × 10−43.20%3/95
Huntington’s Disease Signalling7.49 × 10−41.70%4/229
Overlap represents the percentage of LAP1 interactors integrated in the total number of proteins associated to each specific pathway on the IPA Knowledge Base.
Table
Table 3. Physiological System Development and Function for LAP1 interactors dataset.
Table 3. Physiological System Development and Function for LAP1 interactors dataset.
Namep-Value# Molecules
Skeletal and Muscular System Development and Function3.61 × 10−3–1.82 × 10−67
Tissue Development3.61 × 10−3–1.82 ×10−613
Nervous System Development and Function3.61 × 10−3–3.93 × 10−613
Organ Morphology3.61 × 10−3–3.93 × 10−69
Tissue Morphology3.61 × 10−3–3.93 × 10−616
Table
Table 4. Top Associated Networks for LAP1 interactors dataset.
Table 4. Top Associated Networks for LAP1 interactors dataset.
IDAssociated Network FunctionsScore
1Cell Morphology, Cellular Assembly and Organization, DNA Replication, Recombination, and Repair47
2Cancer, Organismal Injury and Abnormalities, Respiratory Disease32
3RNA Post-Transcriptional Modification, Protein Synthesis, Gene Expression3
4Developmental Disorder, Neurological Disease, Behaviour2
Score attributes a numerical value used to rank networks according to how relevant they are to the genes in the input dataset.
Table
Table 5. Top Toxicological Lists for LAP1 interactors dataset.
Table 5. Top Toxicological Lists for LAP1 interactors dataset.
Namep-ValueOverlap %
Hypoxia-Inducible Factor Signalling2.72 ×10−44.3% 3/70
Mitochondrial Dysfunction3.90 × 10−31.7% 3/176
Cell Cycle: G2/M DNA Damage Checkpoint Regulation3.97 × 10−33.8% 2/52
Cell Cycle: G1/S Checkpoint Regulation6.32 × 10−33.0% 2/66
TR/RXR Activation1.03 × 10−22.4% 2/85
Overlap represents the percentage of LAP1 interactors integrated in the total number of proteins associated to each specific pathway on the IPA Knowledge Base.
Table
Table 6. Molecular and cellular functions for LAP1 interactors dataset.
Table 6. Molecular and cellular functions for LAP1 interactors dataset.
Namep-Value# Molecules
Cell Morphology3.61 × 10−3–3.18 × 10−1118
Cellular Assembly and Organization3.61 × 10−3–3.18 × 10−1120
DNA Replication, Recombination, and Repair3.61 × 10−3–1.34 × 10−1015
Cell Cycle3.61 × 10−3–9.17 × 10−917
Cell Death and Survival3.61 × 10−3–4.21 × 10−720
IPA reinforced the idea that LAP1 might be communicating with the shelterin complex, as is evident in Table 2, where the top two canonical pathways include “Telomerase signalling” and “Telomere Extension by Telomerase”. Furthermore, IPA analysis of physiological system development and function identified “Skeletal and Muscular System Development and Function” as the most relevant (Table 3). This result is in accordance with the previous described functions of LAP1 regarding its association with the Torsin family [77].
Regarding the rank for associated networks in Table 4, the analysis is not limited to molecular interactions. The result delivered the most pertinent functions of LAP1 interactors, which is attributed to “Cell Morphology, Cellular Assembly and Organization, DNA Replication, Recombination, and Repair” (Figure 5).
Membranes 06 00008 g005 1024
Figure 5. Network ID 1. IPA Associated Network Functions: Cell Morphology, Cellular Assembly and Organization, DNA Replication, Recombination, and Repair. Grey nodes represent proteins that are integrated in the initial dataset, while white nodes convey IPA’s supplementary missing proteins.
Figure 5. Network ID 1. IPA Associated Network Functions: Cell Morphology, Cellular Assembly and Organization, DNA Replication, Recombination, and Repair. Grey nodes represent proteins that are integrated in the initial dataset, while white nodes convey IPA’s supplementary missing proteins.
Membranes 06 00008 g005
Additionally, IPA retrieved BRAC1 as the most probable upstream regulator of the LAP1 interactome (Figure S3). This inference is in accordance with the regulation of important players in the proposed network for LAP1 interactors (Figure S3), namely EGRF and ATM concerning DNA damage events and MAD2L1, which might be activated for mitosis regulation with the MCC. Furthermore, the data shown in Table 5 also confirms this hypothesis, with the identification of “Cell Cycle: G2/M DNA Damage Checkpoint Regulation” and “Cell Cycle: G1/S Checkpoint Regulation” as top toxicological attributes.
Lastly, IPA’s analysis of molecular and cellular functions attributed to LAP1’s interactome (Table 6) acknowledges “Cell Morphology” and “Cellular Assembly and Organization” as the most pertinent, followed by “DNA Replication, Recombination, and Repair”, “Cell Cycle” and “Cell Death and Survival”. The networks describing and integrating these functions are available in the Supplementary Figures S4 and S5. By studying these interactions it is possible to deduce the interconnected pathways in which LAP1 might participate, namely, suggesting a crucial role in regulating nuclear morphology, cell cycle progression and cell survival.
Ingenuity pathway analysis confirmed the hypothesis that LAP1, besides the functional associations with lamins and nuclear morphology, might be a crucial player in cell cycle progression and DNA damage responses. These conclusions are in accordance with the prior analysis performed with GO and GeneMANIA.

3. Experimental Section

3.1. Collection of Associated Interactions of LAP1

In order to develop a comprehensive computational analysis of the LAP1 interactome, experimentally detected protein-protein and protein-nucleic acid interactions were extracted from the following public databases: BioGRID [78] (version 3.4.129, consulted 12 October 2015), IntAct [79] (version 4.1.8, consulted 13 October 2015), mentha [80] (version of 13 October 2015I2D [81] (version of 13 October 2015), APID[82] (version of 13 October 2015), HIPPIE [83] (version of 13 October 2015), NCBI [84] (version of 13 October 2015). Additionally, the PSICQUIC web service [85] (version 1.4.5, consulted 14 October 2015) enabled access to multiple PSI-MI compliant resources.
The resulting set of 180 binary interactions includes high and low-throughput data that was later manually curated by consulting the corresponding articles. Despite the worth and accessibility of protein interaction data from online databases, it also comes with limitations. Numerous databases (including the ones used in this analysis) contain interactions based on indirect evidence, for instance, data mining, genetic interactions, metabolic evidence and co-localization data. In this case, the gene set had a dimension that enabled the manual confirmation of each binary interaction. However, when the protein of interest retrieves a greater number of interactors, individually validating the curated interactions, of the results obtained might not be feasible. In these cases, filtering on the website enables the authors to clean the data prior to export. Alternatively, it is possible to import PSI-MI XML or XGMML into Cytoscape and use the filtering tools available. The output from our approach resulted in the identification of 41 LAP1 interactors (Table 1 and Figure 2).

3.2. Gene Ontology Term Enrichment Analysis

Gene Ontology (GO) provides a system of terms to consistently describe and annotate gene products [86]. GO term enrichment analysis (supported by Panther 10.0, released 15 May 2015; analysed 15 October 2015) [76] was performed online using the Search GO data tool (available at [75]) [73] to enquire for GO terms that are over-represented using the annotations regarding biological process and cellular component. The list of genes was pasted into the corresponding box to be analysed, choosing Homo sapiens as the species of genes integrated in the gene set. GO analysis of LAP1 the interactome was only possible by extrapolating the mouse and rat interactions to human. The output retrieved a table that lists significant shared GO terms (or parents of GO terms) used to describe the set of genes that users entered on the previous page, the background frequency, the sample frequency, expected p-value, an indication of over/underrepresentation for each term, and p-value. The statistical analysis included Bonferroni’s correction for multiple testing and a term was reported as enriched if the adjusted p-value is below a significance threshold of 0.05. All the input genes were mapped, however two were unclassified: TOR3A for biological process ontology and HPDL for cellular component. The p-values were corrected for multiple testing using the Bonferroni procedure and transformed by taking the -log10 for easier visualization. The online GO term enrichment service, retrieved from the online platform was stored and analysed as Excel spreadsheet files (Figure 4).

3.3. Software Platforms and Plugins

Data obtained from the online databases already described were stored and analysed as Excel spreadsheet files. The network of LAP1 interactors was built using Cytoscape (3.2.1, freely available online at [87]) [23]. Cytoscape is one of the most popular tools for visualizing, integrating, modelling, and analysing molecular and genetic interaction networks [23].
The GeneMANIA plugin (version 3.4.0) was installed using the Cytoscape App Manager (Cytopscape plugin can be download at [88]) [61]. GeneMANIA is a particularly convenient and user-friendly metasearch platform that generates a highly correlated signalling network centred on the proteins of interest [89]. To determine the interactions between the LAP1 interactors inside the network, the gene set was introduced into GeneMANIA on Cytoscape. Different results can be produced from the same gene list when using GeneMANIA. In this case, by only selecting physical interaction data, the network will only show genes linked if the proteins in question share a binary interaction established through physical experiments [89]. The integrated network obtained after using GeneMANIA was subsequently manually validated for curated interactions with the respective publications, to confirm the binary interactions. With the editing tools offered by Cytoscape this network was designed to obtain the final result presented in Figure 3.
BiNGO is a tool used to determine which Gene Ontology (GO) categories are statistically overrepresented in a set of genes and outputs a network that allows interactive visualization of results mapped by the GO hierarchy [74]. The BiNGO plugin (version 3.0.3) was installed using the Cytoscape App Manager (freely available to download at [90]) [74]. In Cytoscape, BiNGO outputs a network of the significant GO terms using p-values that were corrected for multiple testing using the Bonferroni correction. Each term is size and colour-coded, so that larger nodes have more genes and darker nodes are more significantly enriched. Intermediate terms that are not significant are present as small white nodes [74]. GO terms are organized in a tree-like structure, starting from more general terms at the root (for example, biological regulation) to the most specific at the leaves (for example, the regulation of mitotic cell cycle) distributed across the two main semantic domains—biological process and cellular location. As GO terms might have more than one parent, they are technically structured as a network called a DAG (Figures S1 and S2).
Ingenuity® Pathway Analysis (IPA®, QIAGEN Redwood City,[91]) is a commercially available software program that helps model, analyse and understand complex biological and chemical systems. The Ingenuity Knowledge Base is a repository of expertly curated biological information. Gene identifications of the previously described interactors were imported into the Ingenuity Pathways Analysis (IPA) tool, once more extrapolating the mouse and rat interactions to human and excluding viral connections. Function classifications, signal pathways and interacting networks were constructed and analysed based on the underlying biological evidence from the IPA’s database. The core analysis performed in IPA retrieved seven distinctive functional sets: canonical pathways, upstream regulators, diseases and disorders, molecular and cellular functions, physiological system development and functions, associated network functions and toxicological lists. The output generated through the use of QIAGEN’s Ingenuity Pathway Analysis (IPA®, QIAGEN Redwood City, CA, USA [91]) is depicted in Table 2, Table 3, Table 4 and Table 5 and Figure 5, Figures S3–S6. Right-tailed Fisher’s exact test was used to calculate the significance of each biological function and/or disease assigned, which was reported if the adjusted p-value was below a significance threshold of 0.05.

4. Conclusions

The integration of multiple bioinformatic tools allowed for the development of an intricate study of LAP1 interactors. These analyses attribute functions to LAP1 that have not been previously described. For instance, various DNA damage response proteins that incorporate or regulate the shelterin complex were shown to directly interact with LAP1. This function has been previously associated with INM proteins such as LMNA, SUN1, LAP1 and BAF. Additionally, the previously described association of LAP1 with mitosis was confirmed through the interaction with MCC. Therefore, these and other prospective functions of LAP1 convey the significant role of INM proteins in the regulation of multiple cellular processes and disease conditions.

Supplementary Materials

Figure S1: Significantly enriched Gene Ontology terms from the LAP1 interaction network concerning Biological Processes. Figure S2: Significantly enriched Gene Ontology terms from the LAP1 interaction network concerning Cell Components. Figure S3: Top Upstream Regulators of the LAP1 interactome. Figure S4: Molecular and cellular functions for LAP1 interactors. Figure S5: Merged molecular and cellular functions associated with LAP1 interactors.

Acknowledgments

This work was financed by the Institute for Biomedicine - UID /BIM/04501/2013. We would also like to thank Prof. A. M: M. van Pelt, the AMC Netherlands and the Fundação para a Ciência e Tecnologia of the Ministério da Educação e Ciência (Project, PTDC/BEX-BCM/0494/2013); the COMPETE program, the QREN and the European Union (Fundo Europeu de Desenvolvimento Regional) for the support in this manuscript.

Author Contributions

J.B.S., O.A.B.C.S., S.R. conceived and designed the bioinformatics methodology; J.B.S. performed the bioinformatics analysis; J.B.S. and S.R. analyzed the data; J.B.S., O.A.B.C.S., S.R. wrote and revised the paper.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Dauer, W.T.; Worman, H.J. The Nuclear Envelope as a Signaling Node in Development and Disease. Dev. Cell 2009, 17, 626–638. [Google Scholar] [CrossRef] [PubMed]
  2. Gruenbaum, Y.; Margalit, A.; Goldman, R.D.; Shumaker, D.K.; Wilson, K.L. The nuclear lamina comes of age. Nat. Rev. Mol. Cell Biol. 2005, 6, 21–31. [Google Scholar] [CrossRef] [PubMed]
  3. Burke, B.; Stewart, C.L. Functional architecture of the cell’s nucleus in development, aging, and disease. Curr. Top. Dev. Biol. 2014, 109, 1–52. [Google Scholar] [PubMed]
  4. Schirmer, E.C.; Florens, L.; Guan, T.; Yates, J.R.; Gerace, L. Nuclear membrane proteins with potential disease links found by subtractive proteomics. Science 2003, 301, 1380–1382. [Google Scholar] [CrossRef] [PubMed]
  5. Senior, A.; Gerace, L. Integral membrane proteins specific to the inner nuclear membrane and associated with the nuclear lamina. J. Cell Biol. 1988, 107, 2029–2036. [Google Scholar] [CrossRef] [PubMed]
  6. Martin, L.; Crimaudo, C.; Gerace, L. cDNA cloning and characterization of lamina-associated polypeptide 1C (LAP1C), an integral protein of the inner nuclear membrane. J. Biol. Chem. 1995, 270, 8822–8828. [Google Scholar] [CrossRef] [PubMed]
  7. Kondo, Y.; Kondoh, J.; Hayashi, D.; Ban, T.; Takagi, M.; Kamei, Y.; Tsuji, L.; Kim, J.; Yoneda, Y. Molecular cloning of one isotype of human lamina-associated polypeptide 1s and a topological analysis using its deletion mutants. Biochem. Biophys. Res. Commun. 2002, 294, 770–778. [Google Scholar] [CrossRef]
  8. Santos, M.; Domingues, S.C.; Costa, P.; Muller, T.; Galozzi, S.; Marcus, K.; da Cruz E Silva, E.F.; da Cruz E Silva, O.A.; Rebelo, S. Identification of a Novel Human LAP1 Isoform That Is Regulated by Protein Phosphorylation. PLoS ONE 2014, 9, e113732. [Google Scholar] [CrossRef] [PubMed]
  9. Foisner, R.; Gerace, L. Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation. Cell 1993, 73, 1267–1279. [Google Scholar] [CrossRef]
  10. Gerace, L.; Huber, M.D. Nuclear lamina at the crossroads of the cytoplasm and nucleus. J. Struct. Biol. 2012, 177, 24–31. [Google Scholar] [CrossRef] [PubMed]
  11. Goodchild, R.E.; Dauer, W.T. The AAA+ protein torsinA interacts with a conserved domain present in LAP1 and a novel ER protein. J. Cell Biol. 2005, 168, 855–862. [Google Scholar] [CrossRef] [PubMed]
  12. Ozelius, L.J.; Hewett, J.W.; Page, C.E.; Bressman, S.B.; Kramer, P.L.; Shalish, C.; de Leon, D.; Brin, M.F.; Raymond, D.; Corey, D.P.; et al. The early-onset torsion dystonia gene (DYT1) encodes an ATP-binding protein. Nat. Genet. 1997, 17, 40–48. [Google Scholar] [CrossRef] [PubMed]
  13. Gonzalez-Alegre, P. Aberrant Cellular Behavior of Mutant TorsinA Implicates Nuclear Envelope Dysfunction in DYT1 Dystonia. J. Neurosci. 2004, 24, 2593–2601. [Google Scholar] [CrossRef] [PubMed]
  14. Goodchild, R.E.; Dauer, W.T. Mislocalization to the nuclear envelope: An effect of the dystonia-causing torsinA mutation. Proc. Natl. Acad. Sci. USA 2004, 101, 847–852. [Google Scholar] [CrossRef] [PubMed]
  15. Naismith, T.V.; Dalal, S.; Hanson, P.I. Interaction of torsinA with its major binding partners is impaired by the dystonia-associated DeltaGAG deletion. J. Biol. Chem. 2009, 284, 27866–27874. [Google Scholar] [CrossRef] [PubMed]
  16. Zhao, C.; Brown, R.S.; Chase, A.R.; Eisele, M.R.; Schlieker, C. Regulation of Torsin ATPases by LAP1 and LULL1. Proc. Natl. Acad. Sci. USA 2013, 110, E1545–E1554. [Google Scholar] [CrossRef] [PubMed]
  17. Shin, J.; Méndez-lópez, I.; Wang, Y.; Hays, A.P.; Tanji, K.; Lefkowitch, J.H.; Schulze, P.C.; Worman, H.J.; Dauer, W.T. Lamina-associated Polypeptide-1 Interacts with the Muscular Dystrophy Protein Emerin and is Essential for Skeletal Muscle Maintenance. Dev. Cell 2014, 26, 591–603. [Google Scholar] [CrossRef] [PubMed]
  18. Bione, S.; Maestrini, E.; Rivella, S.; Mancini, M.; Regis, S.; Romeo, G.; Toniolo, D. Identification of a novel X-linked gene responsible for Emery-Dreifuss muscular dystrophy. Nat. Genet. 1994, 8, 323–327. [Google Scholar] [CrossRef] [PubMed]
  19. Santos, M.; Rebelo, S.; van Kleeff, P.J.M.; Kim, C.E.; Dauer, W.T.; Fardilha, M.; da Cruz E Silva, O.A.; da Cruz E Silva, E.F. The nuclear envelope protein, LAP1B, is a novel protein phosphatase 1 substrate. PLoS ONE 2013, 8, e76788. [Google Scholar] [CrossRef] [PubMed]
  20. Kayman-Kurekci, G.; Talim, B.; Korkusuz, P.; Sayar, N.; Sarioglu, T.; Oncel, I.; Sharafi, P.; Gundesli, H.; Balci-Hayta, B.; Purali, N.; et al. Mutation in TOR1AIP1 encoding LAP1B in a form of muscular dystrophy: A novel gene related to nuclear envelopathies. Neuromuscul. Disord. 2014, 24, 624–633. [Google Scholar] [CrossRef] [PubMed]
  21. Dorboz, I.; Coutelier, M.; Bertrand, A.T.; Caberg, J.-H.; Elmaleh-Bergès, M.; Lainé, J.; Stevanin, G.; Bonne, G.; Boespflug-Tanguy, O.; Servais, L. Severe dystonia, cerebellar atrophy, and cardiomyopathy likely caused by a missense mutation in TOR1AIP1. Orphanet J. Rare Dis. 2014, 9, 174. [Google Scholar] [CrossRef] [PubMed]
  22. Rebelo, S.; Edgar, F.; Odete, A.B. Mutation Research/Reviews in Mutation Research Genetic mutations strengthen functional association of LAP1 with DYT1 dystonia and muscular dystrophy. Mutat. Res. Mutat. Res. 2015, 2–7. [Google Scholar]
  23. Shannon, P.; Markiel, A.; Ozier, O.; Baliga, N.S.; Wang, J.T.; Ramage, D.; Amin, N.; Schwikowski, B.; Ideker, T. Cytoscape: A Software Environment for Integrated Models of Biomolecular Interaction Networks. Genome Res. 2003, 13, 2498–2504. [Google Scholar] [CrossRef] [PubMed]
  24. Shin, J.-Y.; Dauer, W.T.; Worman, H.J. Lamina-associated polypeptide 1: Protein interactions and tissue-selective functions. Semin. Cell Dev. Biol. 2014, 29, 164–168. [Google Scholar] [CrossRef] [PubMed]
  25. Abdelmohsen, K.; Srikantan, S.; Yang, X.; Lal, A.; Kim, H.H.; Kuwano, Y.; Galban, S.; Becker, K.G.; Kamara, D.; de Cabo, R.; et al. Ubiquitin-mediated proteolysis of HuR by heat shock. EMBO J. 2009, 28, 1271–1282. [Google Scholar] [CrossRef] [PubMed]
  26. Chu, L.; Su, M.Y.; Maggi, L.B.; Lu, L.; Mullins, C.; Crosby, S.; Huang, G.; Chng, W.J.; Vij, R.; Tomasson, M.H. Multiple myeloma-associated chromosomal translocation activates orphan snoRNA ACA11 to suppress oxidative stress. J. Clin. Invest. 2012, 122, 2793–2806. [Google Scholar] [CrossRef] [PubMed]
  27. Gautier, V.W.; Gu, L.; O’Donoghue, N.; Pennington, S.; Sheehy, N.; Hall, W.W. In vitro nuclear interactome of the HIV-1 Tat protein. Retrovirology 2009, 6, 47. [Google Scholar] [CrossRef] [PubMed]
  28. Wu, W.; Tran, K.C.; Teng, M.N.; Heesom, K.J.; Matthews, D.A.; Barr, J.N.; Hiscox, J.A. The Interactome of the Human Respiratory Syncytial Virus NS1 Protein Highlights Multiple Effects on Host Cell Biology. J. Virol. 2012, 86, 7777–7789. [Google Scholar] [CrossRef] [PubMed]
  29. Lynch, D.T.; Zimmerman, J.S.; Rowe, D.T. Epstein-Barr virus latent membrane protein 2B (LMP2B) co-localizes with LMP2A in perinuclear regions in transiently transfected cells. J. Gen. Virol. 2002, 83, 1025–1035. [Google Scholar] [CrossRef] [PubMed]
  30. Cohen, S.; Etingov, I.; Pante, N. Effect of Viral Infection on the Nuclear Envelope and Nuclear Pore Complex; Elsevier: Amsterdam, The Netherlands, 2012; Volume 299. [Google Scholar]
  31. Huttlin, E.L.; Ting, L.; Bruckner, R.J.; Paulo, J.A.; Gygi, M.P.; Rad, R.; Kolippakkam, D.; Szpyt, J.; Zarraga, G.; Tam, S.; et al. High-Throughput Proteomic Mapping of Human Interaction Networks via Affinity-Purification Mass Spectrometry (Pre-Publication). Available online: http://thebiogrid.org/166968/publication/high-throughput-proteomic-mapping-of-human-interaction-networks-via-affinity-purification-mass-spectrometry.html (accessed on 23 October 2015).
  32. Matsuoka, S.; Ballif, B.A.; Smogorzewska, A.; McDonald, E.R.; Hurov, K.E.; Luo, J.; Bakalarski, C.E.; Zhao, Z.; Solimini, N.; Lerenthal, Y.; et al. ATM and ATR Substrate Analysis Reveals Extensive Protein Networks Responsive to DNA Damage. Science 2007, 316, 1160–1166. [Google Scholar] [CrossRef] [PubMed]
  33. Pestov, N.B.; Ahmad, N.; Korneenko, T.V.; Zhao, H.; Radkov, R.; Schaer, D.; Roy, S.; Bibert, S.; Geering, K.; Modyanov, N.N. Evolution of Na,K-ATPase beta m-subunit into a coregulator of transcription in placental mammals. Proc. Natl. Acad. Sci. USA 2007, 104, 11215–11220. [Google Scholar] [CrossRef] [PubMed]
  34. Huttlin, E.L.; Ting, L.; Bruckner, R.J.; Gebreab, F.; Gygi, M.P.; Szpyt, J.; Tam, S.; Zarraga, G.; Colby, G.; Baltier, K.; et al. The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 2015, 162, 425–440. [Google Scholar] [PubMed]
  35. Hutchins, J.R.A.; Toyoda, Y.; Hegemann, B.; Poser, I.; Hériché, J.-K.; Sykora, M.M.; Augsburg, M.; Hudecz, O.; Buschhorn, B.A.; Bulkescher, J.; et al. Systematic Analysis of Human Protein Complexes Identifies Chromosome Segregation Proteins. Science 2010, 328, 593–599. [Google Scholar] [CrossRef] [PubMed]
  36. Hanson, D.; Stevens, A.; Murray, P.G.; Black, G.C.M.; Clayton, P.E. Identifying biological pathways that underlie primordial short stature using network analysis. J. Mol. Endocrinol. 2014, 52, 333–344. [Google Scholar] [CrossRef] [PubMed]
  37. Tong, J.; Taylor, P.; Moran, M.F. Proteomic Analysis of the Epidermal Growth Factor Receptor (EGFR) Interactome and Post-translational Modifications Associated with Receptor Endocytosis in Response to EGF and Stress. Mol. Cell. Proteomics 2014, 13, 1644–1658. [Google Scholar] [CrossRef] [PubMed]
  38. Havugimana, P.C.; Hart, G.T.; Nepusz, T.; Yang, H.; Turinsky, A.L.; Li, Z.; Wang, P.I.; Boutz, D.R.; Fong, V.; Phanse, S.; et al. Census of Human Soluble Protein Complexes. Cell 2012, 150, 1068–1081. [Google Scholar] [CrossRef] [PubMed]
  39. Kubben, N.; Voncken, J.W.; Demmers, J.; Calis, C.; van Almen, G.; Pinto, Y.M.; Misteli, T. Identification of differential protein interactors of lamin A and progerin. Nucleus 2010, 1, 513–525. [Google Scholar] [CrossRef] [PubMed]
  40. Zhong, N.; Radu, G.; Ju, W.; Brown, W.T. Novel progerin-interactive partner proteins hnRNP E1, EGF, Mel 18, and UBC9 interact with lamin A/C. Biochem. Biophys. Res. Commun. 2005, 338, 855–861. [Google Scholar] [CrossRef] [PubMed]
  41. Roux, K.J.; Kim, D.I.; Raida, M.; Burke, B. A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. J. Cell Biol. 2012, 196, 801–810. [Google Scholar] [CrossRef] [PubMed]
  42. Maison, C.; Pyrpasopoulou, A.; Theodoropoulos, P.A.; Georgatos, S.D. The inner nuclear membrane protein LAP1 forms a native complex with B-type lamins and partitions with spindle-associated mitotic vesicles. EMBO J. 1997, 16, 4839–4850. [Google Scholar] [CrossRef] [PubMed]
  43. Rozenblatt-Rosen, O.; Deo, R.C.; Padi, M.; Adelmant, G.; Calderwood, M.A.; Rolland, T.; Grace, M.; Dricot, A.; Askenazi, M.; Tavares, M.; et al. Interpreting cancer genomes using systematic host network perturbations by tumour virus proteins. Nature 2012, 487, 491–495. [Google Scholar] [CrossRef] [PubMed]
  44. Martin, I.; Kim, J.W.; Lee, B.D.; Kang, H.C.; Xu, J.-C.; Jia, H.; Stankowski, J.; Kim, M.-S.; Zhong, J.; Kumar, M.; et al. Ribosomal protein s15 phosphorylation mediates LRRK2 neurodegeneration in Parkinson’s disease. Cell 2014, 157, 472–485. [Google Scholar] [CrossRef] [PubMed]
  45. Nicholson, J.; Scherl, A.; Way, L.; Blackburn, E.A.; Walkinshaw, M.D.; Ball, K.L.; Hupp, T.R. A systems wide mass spectrometric based linear motif screen to identify dominant in-vivo interacting proteins for the ubiquitin ligase MDM2. Cell. Signal. 2014, 26, 1243–1257. [Google Scholar] [CrossRef] [PubMed]
  46. Emdal, K.B.; Pedersen, A.-K.; Bekker-Jensen, D.B.; Tsafou, K.P.; Horn, H.; Lindner, S.; Schulte, J.H.; Eggert, A.; Jensen, L.J.; Francavilla, C.; et al. Temporal proteomics of NGF-TrkA signaling identifies an inhibitory role for the E3 ligase Cbl-b in neuroblastoma cell differentiation. Sci. Signal. 2015, 8, ra40. [Google Scholar] [CrossRef] [PubMed]
  47. Santos, M. Characterization of novel LAP1 complexes and their relevance in DYT1 dystonia. Ph.D. Thesis, University of Aveiro, Aveiro, Portugal, 2014. [Google Scholar]
  48. Esteves, S.L.; Domingues, S.C.; da Cruz e Silva, O.A.; Fardilha, M.; da Cruz e Silva, E.F. Protein phosphatase 1alpha interacting proteins in the human brain. Omics 2012, 16, 3–17. [Google Scholar] [CrossRef] [PubMed]
  49. Esteves, S.L.C.; Korrodi-Gregório, L.; Cotrim, C.Z.; van Kleeff, P.J.M.; Domingues, S.C.; Da Cruz E Silva, O.A.B.; Fardilha, M.; Da Cruz E Silva, E.F. Protein phosphatase 1γ isoforms linked interactions in the brain. J. Mol. Neurosci. 2013, 50, 179–197. [Google Scholar] [CrossRef] [PubMed]
  50. Lee, O.-H.; Kim, H.; He, Q.; Baek, H.J.; Yang, D.; Chen, L.-Y.; Liang, J.; Chae, H.K.; Safari, A.; Liu, D.; et al. Genome-wide YFP fluorescence complementation screen identifies new regulators for telomere signaling in human cells. Mol. Cell. Proteomics 2011, 10, M110.001628. [Google Scholar] [CrossRef] [PubMed]
  51. Zhu, L.; Millen, L.; Mendoza, J.L.; Thomas, P.J. A unique redox-sensing sensor II motif in TorsinA plays a critical role in nucleotide and partner binding. J. Biol. Chem. 2010, 285, 37271–37280. [Google Scholar] [CrossRef] [PubMed]
  52. Brown, R.S.H.; Zhao, C.; Chase, A.R.; Wang, J.; Schlieker, C. The mechanism of Torsin ATPase activation. Proc. Natl. Acad. Sci. USA 2014, 111, E4822–E4831. [Google Scholar] [CrossRef] [PubMed]
  53. Kim, C.E.; Perez, A.; Perkins, G.; Ellisman, M.H.; Dauer, W.T. A molecular mechanism underlying the neural-specific defect in torsinA mutant mice. Proc. Natl. Acad. Sci. USA 2010, 107, 9861–9866. [Google Scholar] [CrossRef] [PubMed]
  54. Danielsen, J.M.R.; Sylvestersen, K.B.; Bekker-Jensen, S.; Szklarczyk, D.; Poulsen, J.W.; Horn, H.; Jensen, L.J.; Mailand, N.; Nielsen, M.L. Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level. Mol. Cell. Proteomics 2011, 10, M110.003590. [Google Scholar] [CrossRef] [PubMed]
  55. Wagner, S.A.; Beli, P.; Weinert, B.T.; Nielsen, M.L.; Cox, J.; Mann, M.; Choudhary, C. A Proteome-wide, Quantitative Survey of In Vivo Ubiquitylation Sites Reveals Widespread Regulatory Roles. Mol. Cell. Proteomics 2011, 10, M111.013284. [Google Scholar] [CrossRef] [PubMed]
  56. Kim, W.; Bennett, E.J.; Huttlin, E.L.; Guo, A.; Li, J.; Possemato, A.; Sowa, M.E.; Rad, R.; Rush, J.; Comb, M.J.; et al. Systematic and Quantitative Assessment of the Ubiquitin-Modified Proteome. Mol. Cell 2011, 44, 325–340. [Google Scholar] [CrossRef] [PubMed]
  57. Emanuele, M.J.; Elia, A.E.H.; Xu, Q.; Thoma, C.R.; Izhar, L.; Leng, Y.; Guo, A.; Chen, Y.N.; Rush, J.; Hsu, P.W.C.; et al. Global identification of modular cullin-RING ligase substrates. Cell 2011, 147, 459–474. [Google Scholar] [CrossRef] [PubMed]
  58. Povlsen, L.K.; Beli, P.; Wagner, S.A.; Poulsen, S.L.; Sylvestersen, K.B.; Poulsen, J.W.; Nielsen, M.L.; Bekker-Jensen, S.; Mailand, N.; Choudhary, C. Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass. Nat. Cell Biol. 2012, 14, 1089–1098. [Google Scholar] [CrossRef] [PubMed]
  59. Stes, E.; Laga, M.; Walton, A.; Samyn, N.; Timmerman, E.; de Smet, I.; Goormachtig, S.; Gevaert, K. A COFRADIC protocol to study protein ubiquitination. J. Proteome Res. 2014, 13, 3107–3113. [Google Scholar] [CrossRef] [PubMed]
  60. Hewett, J.W.; Zeng, J.; Niland, B.P.; Bragg, D.C.; Breakefield, X.O. Dystonia-causing mutant torsinA inhibits cell adhesion and neurite extension through interference with cytoskeletal dynamics. Neurobiol. Dis. 2006, 22, 98–111. [Google Scholar] [CrossRef] [PubMed]
  61. Montojo, J.; Zuberi, K.; Rodriguez, H.; Kazi, F.; Wright, G.; Donaldson, S.L.; Morris, Q.; Bader, G.D. GeneMANIA Cytoscape plugin: Fast gene function predictions on the desktop. Bioinformatics 2010, 26, 2927–2928. [Google Scholar] [CrossRef] [PubMed]
  62. Schirmer, E.C.; Foisner, R. Proteins that associate with lamins: Many faces, many functions. Exp. Cell Res. 2007, 313, 2167–2179. [Google Scholar] [CrossRef] [PubMed]
  63. Yang, M.; Li, B.; Tomchick, D.R.; Machius, M.; Rizo, J.; Yu, H.; Luo, X. p31comet blocks Mad2 activation through structural mimicry. Cell 2007, 131, 744–755. [Google Scholar] [CrossRef] [PubMed]
  64. Chao, W.C.H.; Kulkarni, K.; Zhang, Z.; Kong, E.H.; Barford, D. Structure of the mitotic checkpoint complex. Nature 2012, 484, 208–213. [Google Scholar] [CrossRef] [PubMed]
  65. Santos, M.; Costa, P.; Martins, F.; da Cruz e Silva, E.F.; da Cruz e Silva, O.A.B.; Rebelo, S. LAP1 is a crucial protein for the maintenance of the nuclear envelope structure and cell cycle progression. Mol. Cell. Biochem. 2015, 399, 143–153. [Google Scholar] [CrossRef] [PubMed]
  66. Chi, Y.; Welcker, M.; Hizli, A.A.; Posakony, J.J.; Aebersold, R.; Clurman, B.E. Identification of CDK2 substrates in human cell lysates. Genome Biol. 2008, 9, R149. [Google Scholar] [CrossRef] [PubMed]
  67. Olsen, J.V.; Vermeulen, M.; Santamaria, A.; Kumar, C.; Miller, M.L.; Jensen, L.J.; Gnad, F.; Cox, J.; Jensen, T.S.; Nigg, E.A.; et al. Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci. Signal. 2010, 3, ra3. [Google Scholar] [CrossRef] [PubMed]
  68. Blethrow, J.D.; Glavy, J.S.; Morgan, D.O.; Shokat, K.M. Covalent capture of kinase-specific phosphopeptides reveals Cdk1-cyclin B substrates. Proc. Natl. Acad. Sci. USA 2008, 105, 1442–1447. [Google Scholar] [CrossRef] [PubMed]
  69. Xu, Y.; Shao, Y.; Voorhees, J.J.; Fisher, G.J. Ultraviolet Irradiation-Induces Epidermal Growth Factor Receptor (EGFR) Nuclear Translocation in Human Keratinocytes. J. Cell. Biochem. 2009, 107, 873–880. [Google Scholar] [CrossRef] [PubMed]
  70. De Lange, T. Shelterin: The protein complex that shapes and safeguards human telomeres. Genes Dev. 2005, 19, 2100–2110. [Google Scholar] [CrossRef] [PubMed]
  71. Silverman, J.; Takai, H.; Buonomo, S.B.C.; Eisenhaber, F.; de Lange, T. Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM and 53BP1 and functions in the S-phase checkpoint. Genes Dev. 2004, 18, 2108–2019. [Google Scholar] [CrossRef] [PubMed]
  72. Gruenbaum, Y.; Foisner, R. Lamins: Nuclear Intermediate Filament Proteins with Fundamental Functions in Nuclear Mechanics and Genome Regulation. Annu. Rev. Biochem. 2015, 84, 131–164. [Google Scholar] [CrossRef] [PubMed]
  73. Gene, T.; Consortium, O.; Gene, T.; Go, O. Gene Ontology Consortium: Going forward. Nucleic Acids Res. 2015, 43, D1049–D1056. [Google Scholar]
  74. Maere, S.; Heymans, K.; Kuiper, M. BiNGO: A Cytoscape plugin to assess overrepresentation of gene ontology categories in biological networks. Bioinformatics 2005, 21, 3448–3449. [Google Scholar] [CrossRef] [PubMed]
  75. Gene Ontology Consortium. Available online: http://geneontology.org/ (accessed on 23 October 2015).
  76. Mi, H.; Muruganujan, A.; Thomas, P.D. PANTHER in 2013: Modeling the evolution of gene function, and other gene attributes, in the context of phylogenetic trees. Nucleic Acids Res. 2013, 41, D377–D386. [Google Scholar] [CrossRef] [PubMed]
  77. Santos, M.; Rebelo, S.; da Cruz e Silva, E.F.; da Cruz e Silva, O.A.B. DYT1 dystonia-associated mutant affects cytoskeletal dynamics. Microsc. Microanal. 2015, 21, 26–27. [Google Scholar] [CrossRef]
  78. Stark, C. BioGRID: A general repository for interaction datasets. Nucleic Acids Res. 2006, 34, D535–D539. [Google Scholar] [CrossRef] [PubMed]
  79. Orchard, S.; Ammari, M.; Aranda, B.; Breuza, L.; Briganti, L.; Broackes-Carter, F.; Campbell, N.H.; Chavali, G.; Chen, C.; Del-Toro, N.; et al. The MIntAct project—IntAct as a common curation platform for 11 molecular interaction databases. Nucleic Acids Res. 2014, 42, 358–363. [Google Scholar] [CrossRef] [PubMed]
  80. Calderone, A.; Castagnoli, L.; Cesareni, G. Mentha: A Resource for Browsing Integrated Protein-Interaction Networks. Nat. Methods 2013, 10, 690–691. [Google Scholar] [CrossRef] [PubMed]
  81. Brown, K.R.; Jurisica, I. Online Predicted Human Interaction Database. Bioinformatics 2005, 21, 2076–2082. [Google Scholar] [CrossRef] [PubMed]
  82. Prieto, C.; De Las Rivas, J. APID: Agile Protein Interaction DataAnalyzer. Nucleic Acids Res. 2006, 34, W298–W302. [Google Scholar] [CrossRef] [PubMed]
  83. Schaefer, M.H.; Fontaine, J.F.; Vinayagam, A.; Porras, P.; Wanker, E.E.; Andrade-Navarro, M.A. Hippie: Integrating protein interaction networks with experiment based quality scores. PLoS ONE 2012, 7, 1–8. [Google Scholar] [CrossRef] [PubMed]
  84. Maglott, D.; Ostell, J.; Pruitt, K.D.; Tatusova, T. Entrez gene: Gene-centered information at NCBI. Nucleic Acids Res. 2007, 35, D26–D31. [Google Scholar] [CrossRef] [PubMed]
  85. Del-Toro, N.; Dumousseau, M.; Orchard, S.; Jimenez, R.C.; Galeota, E.; Launay, G.; Goll, J.; Breuer, K.; Ono, K.; Salwinski, L.; et al. A new reference implementation of the PSICQUIC web service. Nucleic Acids Res. 2013, 41, W601–W606. [Google Scholar] [CrossRef] [PubMed]
  86. Ashburner, M.; Ball, C.A.; Blake, J.A.; Botstein, D.; Butler, H.; Cherry, J.M.; Davis, A.P.; Dolinski, K.; Dwight, S.S.; Eppig, J.T.; et al. Gene ontology: tool for the unification of biology. Nat. Genet. 2000, 25, 25–29. [Google Scholar] [CrossRef] [PubMed]
  87. Cytoscape Consortium. Available online: http://www.cytoscape.org/ (accessed on 23 October 2015).
  88. GeneMANIA Cytoscape plugin. Available online: http://apps.cytoscape.org/apps/genemania (accessed on 23 October 2015).
  89. Warde-Farley, D.; Donaldson, S.L.; Comes, O.; Zuberi, K.; Badrawi, R.; Chao, P.; Franz, M.; Grouios, C.; Kazi, F.; Lopes, C.T.; et al. The GeneMANIA prediction server: Biological network integration for gene prioritization and predicting gene function. Nucleic Acids Res. 2010, 38, W214–W220. [Google Scholar] [CrossRef] [PubMed]
  90. Maere, S. BiNGO Cytoscape plugin. Available online: http://apps.cytoscape.org/apps/bingo (accessed on 23 October 2015).
  91. QIAGEN Ingenuity Pathway Analysis. Available online: www.qiagen.com/ingenuity (accessed on 23 October 2015).

Share and Cite

MDPI and ACS Style

Serrano, J.B.; Da Cruz e Silva, O.A.B.; Rebelo, S. Lamina Associated Polypeptide 1 (LAP1) Interactome and Its Functional Features. Membranes 2016, 6, 8. https://doi.org/10.3390/membranes6010008

AMA Style

Serrano JB, Da Cruz e Silva OAB, Rebelo S. Lamina Associated Polypeptide 1 (LAP1) Interactome and Its Functional Features. Membranes. 2016; 6(1):8. https://doi.org/10.3390/membranes6010008

Chicago/Turabian Style

Serrano, Joana B., Odete A. B. Da Cruz e Silva, and Sandra Rebelo. 2016. "Lamina Associated Polypeptide 1 (LAP1) Interactome and Its Functional Features" Membranes 6, no. 1: 8. https://doi.org/10.3390/membranes6010008

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop