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Immobilization of Mucor miehei Lipase onto Macroporous Aminated Polyethersulfone Membrane for Enzymatic Reactions
Chemistry Study Program, Institute Technology of Bandung, Jalan Ganesha No. 10 Bandung 40132, Indonesia
Department of Polymer Chemistry & Zernike Institute for Advanced Materials, University of Groningen, Nijenborg 4, 9747 AG, The Netherlands
* Author to whom correspondence should be addressed.
Received: 6 February 2012; in revised form: 28 March 2012 / Accepted: 29 March 2012 / Published: 12 April 2012
Abstract: Immobilization of enzymes is one of the most promising methods in enzyme performance enhancement, including stability, recovery, and reusability. However, investigation of suitable solid support in enzyme immobilization is still a scientific challenge. Polyethersulfone (PES) and aminated PES (PES–NH2) were successfully synthesized as novel materials for immobilization. Membranes with various pore sizes (from 10–600 nm) based on synthesized PES and PES–NH2 polymers were successfully fabricated to be applied as bioreactors to increase the immobilized lipase performances. The influence of pore sizes, concentration of additives, and the functional groups that are attached on the PES backbone on enzyme loading and enzyme activity was studied. The largest enzyme loading was obtained by Mucor miehei lipase immobilized onto a PES–NH2 membrane composed of 10% of PES–NH2, 8% of dibutyl phthalate (DBP), and 5% of polyethylene glycol (PEG) (872.62 µg/cm2). Hydrolytic activity of the immobilized lipases indicated that the activities of biocatalysts are not significantly decreased by immobilization. From the reusability test, the lipase immobilized onto PES–NH2 showed a better constancy than the lipase immobilized onto PES (the percent recovery of the activity of the lipases immobilized onto PES–NH2 and PES are 97.16% and 95.37%, respectively), which indicates that this novel material has the potential to be developed as a bioreactor for enzymatic reactions.
Keywords: aminated PES; solid support; Mucor miehei; enzymatic reactions; lipase immobilization
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MDPI and ACS Style
Handayani, N.; Loos, K.; Wahyuningrum, D.; Buchari; Zulfikar, M.A. Immobilization of Mucor miehei Lipase onto Macroporous Aminated Polyethersulfone Membrane for Enzymatic Reactions. Membranes 2012, 2, 198-213.
Handayani N, Loos K, Wahyuningrum D, Buchari, Zulfikar MA. Immobilization of Mucor miehei Lipase onto Macroporous Aminated Polyethersulfone Membrane for Enzymatic Reactions. Membranes. 2012; 2(2):198-213.
Handayani, Nurrahmi; Loos, Katja; Wahyuningrum, Deana; Buchari; Zulfikar, Muhammad Ali. 2012. "Immobilization of Mucor miehei Lipase onto Macroporous Aminated Polyethersulfone Membrane for Enzymatic Reactions." Membranes 2, no. 2: 198-213.