Improved Induction of Anti-Melanoma T Cells by Adenovirus-5/3 Fiber Modification to Target Human DCs
AbstractTo mount a strong anti-tumor immune response, non T cell inflamed (cold) tumors may require combination treatment encompassing vaccine strategies preceding checkpoint inhibition. In vivo targeted delivery of tumor-associated antigens (TAA) to dendritic cells (DCs), relying on the natural functions of primary DCs in situ, represents an attractive vaccination strategy. In this study we made use of a full-length MART-1 expressing C/B-chimeric adenoviral vector, consisting of the Ad5 capsid and the Ad3 knob (Ad5/3), which we previously showed to selectively transduce DCs in human skin and lymph nodes. Our data demonstrate that chimeric Ad5/3 vectors encoding TAA, and able to target human DCs in situ, can be used to efficiently induce expansion of functional tumor-specific CD8+ effector T cells, either from a naïve T cell pool or from previously primed T cells residing in the melanoma-draining sentinel lymph nodes (SLN). These data support the use of Ad3-knob containing viruses as vaccine vehicles for in vivo delivery. “Off-the-shelf” DC-targeted Ad vaccines encoding TAA could clearly benefit future immunotherapeutic approaches. View Full-Text
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Chondronasiou, D.; Eisden, T.-J.T.H.D.; Stam, A.G.M.; Matthews, Q.L.; Icyuz, M.; Hooijberg, E.; Dmitriev, I.; Curiel, D.T.; de Gruijl, T.D.; van de Ven, R. Improved Induction of Anti-Melanoma T Cells by Adenovirus-5/3 Fiber Modification to Target Human DCs. Vaccines 2018, 6, 42.
Chondronasiou D, Eisden T-JTHD, Stam AGM, Matthews QL, Icyuz M, Hooijberg E, Dmitriev I, Curiel DT, de Gruijl TD, van de Ven R. Improved Induction of Anti-Melanoma T Cells by Adenovirus-5/3 Fiber Modification to Target Human DCs. Vaccines. 2018; 6(3):42.Chicago/Turabian Style
Chondronasiou, Dafni; Eisden, Tracy-Jane T.H.D.; Stam, Anita G.M.; Matthews, Qiana L.; Icyuz, Mert; Hooijberg, Erik; Dmitriev, Igor; Curiel, David T.; de Gruijl, Tanja D.; van de Ven, Rieneke. 2018. "Improved Induction of Anti-Melanoma T Cells by Adenovirus-5/3 Fiber Modification to Target Human DCs." Vaccines 6, no. 3: 42.
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