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Vaccines 2017, 5(4), 31; doi:10.3390/vaccines5040031

Newcastle Disease Virus Vectored Bivalent Vaccine against Virulent Infectious Bursal Disease and Newcastle Disease of Chickens

1
Recombinant DNA Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar 243 122, UP, India
2
Immunology Section, Indian Veterinary Research Institute, Izatnagar 243 122, UP, India
3
Institute of Marine and Environmental Technology, University of Maryland Baltimore County, Baltimore, MD 21202, USA
Present Address: Department of Microbiology, College of Veterinary and Animal Sciences, Parbhani 431 402, Maharashtra, India.
*
Authors to whom correspondence should be addressed.
Academic Editor: Shiladitya DasSarma
Received: 14 August 2017 / Revised: 13 September 2017 / Accepted: 22 September 2017 / Published: 26 September 2017
(This article belongs to the Special Issue Microbial Antigen Delivery)
View Full-Text   |   Download PDF [2898 KB, uploaded 27 September 2017]   |  

Abstract

Newcastle disease virus (NDV) strain F is a lentogenic vaccine strain used for primary vaccination in day-old chickens against Newcastle disease (ND) in India and Southeast Asian countries. Recombinant NDV-F virus and another recombinant NDV harboring the major capsid protein VP2 gene of a very virulent infectious bursal disease virus (IBDV); namely rNDV-F and rNDV-F/VP2, respectively, were generated using the NDV F strain. The rNDV-F/VP2 virus was slightly attenuated, as compared to the rNDV-F virus, as evidenced from the mean death time and intracerebral pathogenicity index analysis. This result indicates that rNDV-F/VP2 behaves as a lentogenic virus and it is stable even after 10 serial passages in embryonated chicken eggs. When chickens were vaccinated with the rNDV F/VP2, it induced both humoral and cell mediated immunity, and was able to confer complete protection against very virulent IBDV challenge and 80% protection against virulent NDV challenge. These results suggest that rNDV-F could be an effective and inherently safe vaccine vector. Here, we demonstrate that a bivalent NDV-IBDV vaccine candidate generated by reverse genetics method is safe, efficacious and cost-effective, which will greatly aid the poultry industry in developing countries. View Full-Text
Keywords: Newcastle disease virus vector; IBDV-VP2 protein; bivalent vaccine candidate; humoral and cell mediated immune responses; protection efficacy Newcastle disease virus vector; IBDV-VP2 protein; bivalent vaccine candidate; humoral and cell mediated immune responses; protection efficacy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Dey, S.; Chellappa, M.M.; Pathak, D.C.; Gaikwad, S.; Yadav, K.; Ramakrishnan, S.; Vakharia, V.N. Newcastle Disease Virus Vectored Bivalent Vaccine against Virulent Infectious Bursal Disease and Newcastle Disease of Chickens. Vaccines 2017, 5, 31.

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