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Evaluation of Different DNA Vaccines against Porcine Reproductive and Respiratory Syndrome (PRRS) in Pigs
Umbria and Marche Experimental Zooprophylaxis Institute, via Gaetano Salvemini 1, Perugia 06126, Italy
Genetic Immunization Unit, Department of Biology, University of Camerino, Via Camerini 1, Camerino 62032, Italy
Lombardia and Emilia-Romagna Experimental Zooprophylaxis Institute, Via Antonio Bianchi, 7/9, Brescia 25124, Italy
Pathology Unit, Department of Veterinary Sciences, Faculty of Veterinary Medicine, University of Parma, Via del Taglio 10, Parma 43100, Italy
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 30 July 2013; in revised form: 10 September 2013 / Accepted: 9 October 2013 / Published: 18 October 2013
Abstract: In veterinary medicine, there have been different experiences with the plasmid DNA vaccination. In this area and with the hypothesis to demonstrate the effectiveness of different plasmids encoding porcine respiratory and reproductive syndrome (PRRS), five DNA vaccines against PRRS were evaluated for their innocuity and efficacy in pigs. Eighteen animals were divided into five groups which were injected with five (A, B, C, D, E) different DNA vaccines. Albeit, none of the proposed vaccines were able to protect the animals against PRRS virus. Only vaccines A and B were able to reduce the clinical signs of the infection. ELISA IgM were detected 30 days after the first vaccination in the pigs injected by Vaccine A or B. ELISA IgG were detected 90 days after the first vaccination in the pigs injected by Vaccine B or C. Neutralizing antibody were detected Post Challenge Days 61 (PCD) in all groups. In the pigs inoculated with Vaccine C, IFN-g were detected 90 days after first vaccination, and after challenge exposure they increased. In the other groups, the IFN-g were detected after challenge infection. Pigs injected with each of the vaccines A, B, C, D and E showed a significantly higher level of CD4−CD8+ lymphocytes (p < 0.001) after infection in comparison with their controls.
Keywords: pigs; DNA vaccines; PRRS; ORF4; ORF5; CpG; UbilacI; NeuL
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Petrini, S.; Ramadori, G.; Villa, R.; Borghetti, P.; de Angelis, E.; Cantoni, A.M.; Corradi, A.; Amici, A.; Ferrari, M. Evaluation of Different DNA Vaccines against Porcine Reproductive and Respiratory Syndrome (PRRS) in Pigs. Vaccines 2013, 1, 463-480.
Petrini S, Ramadori G, Villa R, Borghetti P, de Angelis E, Cantoni AM, Corradi A, Amici A, Ferrari M. Evaluation of Different DNA Vaccines against Porcine Reproductive and Respiratory Syndrome (PRRS) in Pigs. Vaccines. 2013; 1(4):463-480.
Petrini, Stefano; Ramadori, Giorgio; Villa, Riccardo; Borghetti, Paolo; de Angelis, Elena; Cantoni, Anna M.; Corradi, Attilio; Amici, Augusto; Ferrari, Maura. 2013. "Evaluation of Different DNA Vaccines against Porcine Reproductive and Respiratory Syndrome (PRRS) in Pigs." Vaccines 1, no. 4: 463-480.