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Antioxidants 2017, 6(3), 67; doi:10.3390/antiox6030067

Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID—A Fully Validated Approach

1
General Chemical Lab of Research and Analysis, Timfristou 181, 35100 Lamia, Greece
2
Lamia Laboratory, Karaiskaki 85, 35100 Lamia, Greece
3
Laboratory of Food Chemistry, Department of Chemistry, National and Kapodistrian University of Athens Panepistimiopolis Zografou, 15771 Athens, Greece
*
Author to whom correspondence should be addressed.
Received: 26 July 2017 / Revised: 15 August 2017 / Accepted: 18 August 2017 / Published: 22 August 2017
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Abstract

A simple method for the determination of caffeine in coffee grains by GC-FID (Gas Chromatography-Flame Ionisation Detector) is presented in the current work. The method was fully validated according to ISO (International Organization for Standardization) 17025 requirements and European Commission regulations. The accuracy, as provided by recovery experiments, was higher than 93%, and the precision, as provided by the (%) relative standard deviation under reproducibility conditions, was lower than 5%. A vast number of independent parameters that lead in the increase of uncertainty of methods were investigated. The analysis was performed without use of an internal standard, which was proven to be reliable according to several validation methods. The method was applied in real samples, and possible health claims were investigated. View Full-Text
Keywords: caffeine; uncertainty calculation; ISO 17025; Guatemala coffee grains; GC-FID caffeine; uncertainty calculation; ISO 17025; Guatemala coffee grains; GC-FID
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Pasias, I.N.; Kiriakou, I.; Proestos, C. Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID—A Fully Validated Approach. Antioxidants 2017, 6, 67.

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