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Antioxidants 2017, 6(3), 57; doi:10.3390/antiox6030057

Chemical Analysis of Astragali Complanati Semen and Its Hypocholesterolemic Effect Using Serum Metabolomics Based on Gas Chromatography-Mass Spectrometry

1
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China
2
State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Shenzhen 518057, China
3
Food Safety and Technology Research Centre, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China
4
Department of Food and Health Sciences, Faculty of Science and Technology, Technological and Higher Education Institute of Hong Kong, Hong Kong, China
5
Clinical Laboratory, Shenzhen Nanshan Center for Chronic Disease Control, Shenzhen 518000, China
6
The Center Hospital of Hengyang, Hengyang 421001, China
*
Authors to whom correspondence should be addressed.
Received: 15 June 2017 / Revised: 17 July 2017 / Accepted: 18 July 2017 / Published: 21 July 2017
(This article belongs to the Special Issue Bioactive Phenolic Compounds)
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Abstract

The hypocholesterolemic protective effect of the dried seed of Astragalus complanatus (ACS) was investigated in rats fed with normal diet, high cholesterol diet (HCD), and HCD plus 70% ethanol extract of ACS (600 mg/kg/day) by oral gavage for four weeks. ACS extract was tested to be rich in antioxidants, which may be contributed to its high content of phenolic compounds. Consumption of ACS remarkably suppressed the elevated total cholesterol (p < 0.01) and LDL-C (p < 0.001) induced by HCD. Chemical constituents of ACS extract were analyzed by ultra-performance liquid chromatography coupled with electrospray ionization orbitrap mass spectrometry and the results showed that the ACS extract mainly consisted of phenolic compounds including flavonoids and flavonoid glycosides. In addition, based on the serum fatty acid profiles, elucidated using gas chromatography-mass spectrometry, free and esterified fatty acids including docosapentaenoic acid, adrenic acid, dihomo-γ-linolenic acid and arachidonic acid were regulated in ACS treatment group. Western blot results further indicated the protein expression of peroxisome proliferator-activated receptor alpha (PPARα) (p < 0.05) in liver was upregulated in ACS treatment group. To conclude, our results clearly demonstrated that ACS provides beneficial effect on lowering HCD associated detrimental change. View Full-Text
Keywords: Astragali Complanati Semen; serum metabolomics; hypercholesterolemia; mass spectrometry Astragali Complanati Semen; serum metabolomics; hypercholesterolemia; mass spectrometry
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MDPI and ACS Style

Sham, T.T.; Zhang, H.; Mok, D.K.W.; Chan, S.W.; Wu, J.; Tang, S.; Chan, C.O. Chemical Analysis of Astragali Complanati Semen and Its Hypocholesterolemic Effect Using Serum Metabolomics Based on Gas Chromatography-Mass Spectrometry. Antioxidants 2017, 6, 57.

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