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Antioxidants 2016, 5(4), 39; doi:10.3390/antiox5040039

Antioxidant Activity of Oat Proteins Derived Peptides in Stressed Hepatic HepG2 Cells

Food Science and Nutrition, Department of Chemistry, Carleton University, Ottawa, ON K1S 5B6, Canada
Department of Biology and Institute of Biochemistry, Carleton University, Ottawa, ON K1S 5B6, Canada
Author to whom correspondence should be addressed.
Academic Editor: David Burritt
Received: 2 August 2016 / Revised: 14 October 2016 / Accepted: 19 October 2016 / Published: 20 October 2016
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The purpose of this study was to determine, for the first time, antioxidant activities of seven peptides (P1–P7) derived from hydrolysis of oat proteins in a cellular model. In the oxygen radical absorbance capacity (ORAC) assay, it was found that P2 had the highest radical scavenging activity (0.67 ± 0.02 µM Trolox equivalent (TE)/µM peptide) followed by P5, P3, P6, P4, P1, and P7 whose activities were between 0.14–0.61 µM TE/µM). In the hepatic HepG2 cells, none of the peptides was cytotoxic at 20–300 µM. In addition to having the highest ORAC value, P2 was also the most protective (29% increase in cell viability) against 2,2′-azobis(2-methylpropionamidine) dihydrochloride -induced oxidative stress. P1, P6, and P7 protected at a lesser extent, with an 8%–21% increase viability of cells. The protection of cells was attributed to several factors including reduced production of intracellular reactive oxygen species, increased cellular glutathione, and increased activities of three main endogenous antioxidant enzymes. View Full-Text
Keywords: oat peptides; cytoprotection; antioxidant enzymes; HepG2 cells oat peptides; cytoprotection; antioxidant enzymes; HepG2 cells

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Du, Y.; Esfandi, R.; Willmore, W.G.; Tsopmo, A. Antioxidant Activity of Oat Proteins Derived Peptides in Stressed Hepatic HepG2 Cells. Antioxidants 2016, 5, 39.

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