Next Article in Journal
Erratum: Hunaefi, D., et al. The Effect of Lactobacillus plantarum ATCC 8014 and Lactobacillus acidophilus NCFM Fermentation on Antioxidant Properties of Selected in Vitro Sprout Culture of Orthosiphon aristatus (Java Tea) as a Model Study. Antioxidants 2012, 1, 4–32
Next Article in Special Issue
Olive Tree (Olea europeae L.) Leaves: Importance and Advances in the Analysis of Phenolic Compounds
Previous Article in Journal / Special Issue
Phytochemical, Antioxidant and Anti-Cancer Properties of Euphorbia tirucalli Methanolic and Aqueous Extracts
Article Menu

Export Article

Open AccessArticle
Antioxidants 2015, 4(4), 662-680; doi:10.3390/antiox4040662

Identification and Antioxidant Activity of the Extracts of Eugenia uniflora Leaves. Characterization of the Anti-Inflammatory Properties of Aqueous Extract on Diabetes Expression in an Experimental Model of Spontaneous Type 1 Diabetes (NOD Mice)

1
Laboratory of Translational Immunology, Faculty of Medical Sciences, University of Campinas, Rua Vital Brazil, 300, Cidade Universitária Zeferino Vaz, 13083-888 Campinas-SP, Brazil
2
Department of Food and Nutrition, School of Food Engineering, University of Campinas (FEA-Unicamp), Rua Monteiro Lobato, 80, Cidade Universitária Zeferino Vaz, 13083-862 Campinas-SP, Brazil
3
Agronomic Institute of Campinas, Theodureto de Almeida Camargo, 1500, Vila Nova, 13012-970 Campinas-SP, Brazil
4
Department of Internal Medicine, Faculty of Medical Sciences, University of Campinas, Rua Tessália Vieira de Camargo, 126, Cidade Universitária Zeferino Vaz, 13083-887 Campinas-SP, Brazil
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editors: Antonio Segura-Carretero and David Arráez-Román
Received: 17 June 2015 / Revised: 4 September 2015 / Accepted: 8 September 2015 / Published: 9 October 2015
(This article belongs to the Special Issue Analytical Determination of Polyphenols)
View Full-Text   |   Download PDF [739 KB, uploaded 9 October 2015]   |  

Abstract

Medical and folklore reports suggest that Eugenia uniflora (E. uniflora) is a functional food that contains numerous compounds in its composition, with anti-inflammatory, antioxidant and anti-diabetic effects. In the present study, we investigated the best solvents (water, ethanol and methanol/acetone) for extracting bioactive compounds of E. uniflora leaves, assessing total phenols and the antioxidant activity of the extracts by 2,2-Diphenyl-1-picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP), 2,2′-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and Oxygen Radical Absorbance Capacity (ORAC) assays, identifying hydrolysable tannins and three phenolic compounds (ellagic acid, gallic acid and rutin) present in the leaves. In addition, we evaluated the incidence of diabetes, degree of insulitis, serum insulin, hepatic glutathione and tolerance test glucose in non-obese diabetic (NOD) mice. Our results suggest that the aqueous extract presents antioxidant activity and high total phenols, which were used as a type 1 diabetes mellitus (DM-1) treatment in NOD mice. We verified that the chronic consumption of aqueous extract reduces the inflammatory infiltrate index in pancreatic islets, maintaining serum insulin levels and hepatic glutathione, and reducing serum lipid peroxidation as well as the risk for diabetes. View Full-Text
Keywords: Eugenia uniflora; type 1 diabetes mellitus; NOD mice; antioxidant Eugenia uniflora; type 1 diabetes mellitus; NOD mice; antioxidant
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Schumacher, N.S.G.; Colomeu, T.C.; de Figueiredo, D.; Carvalho, V.C.; Cazarin, C.B.B.; Prado, M.A.; Meletti, L.M.M.; Zollner, R.L. Identification and Antioxidant Activity of the Extracts of Eugenia uniflora Leaves. Characterization of the Anti-Inflammatory Properties of Aqueous Extract on Diabetes Expression in an Experimental Model of Spontaneous Type 1 Diabetes (NOD Mice). Antioxidants 2015, 4, 662-680.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Antioxidants EISSN 2076-3921 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top