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Antioxidants 2015, 4(2), 281-292; doi:10.3390/antiox4020281

In Vitro Cultivars of Vaccinium corymbosum L. (Ericaceae) are a Source of Antioxidant Phenolics

Laboratorio de Fisiología y Biotecnología Vegetal, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile. L. B. O'Higgins Ave. #3363, Estación Central, Santiago of Chile, 9170022, Chile
Ph.D. Program in Biotechnology.
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Authors to whom correspondence should be addressed.
Academic Editors: Antonio Segura-Carretero and David Arráez-Román
Received: 15 November 2014 / Revised: 31 March 2015 / Accepted: 2 April 2015 / Published: 9 April 2015
(This article belongs to the Special Issue Analytical Determination of Polyphenols)
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Abstract

The antioxidant activity and phenolic composition of six in vitro cultured blueberry seedlings were determined. Extracts were prepared in 85% ethanol from 30 days old in vitro cultured plants and used to evaluate the antioxidant capacities that included Ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazin (DPPH•) scavenging ability, total polyphenols (TP) and the partial phenolic composition performed by high performance liquid chromatography with diode array detector (HPLC-DAD), liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS (ESI-QqQ)). All ethanolic extracts from in vitro blueberry cultivars displayed antioxidant activity, with Legacy, Elliott and Bluegold cultivars being the most active. In addition, we observed a positive correlation between phenolic content and antioxidant activity. Our results suggest that the antioxidant activity of the extracts is related to the content of chlorogenic acid myricetin, syringic acid and rutin, and tissue culture of blueberry seedlings is a good tool to obtain antioxidant extracts with reproducible profile of compounds. View Full-Text
Keywords: high-bush blueberry in vitro cultivars; HPLC-DAD and LC-MS/MS; DPPH• and FRAP; phenolic composition high-bush blueberry in vitro cultivars; HPLC-DAD and LC-MS/MS; DPPH• and FRAP; phenolic composition
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Contreras, R.A.; Köhler, H.; Pizarro, M.; Zúiga, G.E. In Vitro Cultivars of Vaccinium corymbosum L. (Ericaceae) are a Source of Antioxidant Phenolics. Antioxidants 2015, 4, 281-292.

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