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Antioxidants 2014, 3(1), 129-143; doi:10.3390/antiox3010129

Green Tea Extract Induces the Resistance of Caenorhabditis elegans against Oxidative Stress

1 Department of Biotechnology, Faculty of Applied Sciences, University of Kalamoon, Damascus countryside, Deir Attiah, Syria 2 Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg 96120, Germany
* Author to whom correspondence should be addressed.
Received: 20 January 2014 / Revised: 13 February 2014 / Accepted: 19 February 2014 / Published: 4 March 2014
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Epidemiological studies on the effects of green tea consumption (Camellia sinensis) have demonstrated a reduction for the risk of age-related diseases. The investigation of the in vivo and in vitro antioxidant properties of an aqueous extract of green tea (GTE) was the aim of the current study. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and superoxide anion radical (O2•−) assays were used to estimate the GTE antioxidant activity. To investigate the protective effects of GTE against oxidative stress, wild-type N2 and transgenic strains (TJ374, hsp-16.2/GFP) of the model organism, Caenorhabditis elegans (C. elegans), were chosen. In the current study, the following catechins were identified by LC/ESI-MS: catechin, epicatechin, epicatechin gallate, gallocatechin, epigallocatechin and epigallocatechin gallate. GTE exhibited a free radical scavenging activity of DPPH and O2•− with IC50 8.37 and 91.34 µg/mL, respectively. In the C. elegans strain (TJ374, hsp-16.2/GFP), the expression of hsp-16.2/GFP was induced by a nonlethal dose of juglone, and the fluorescence density of hsp-16.2/GFP was measured. The hsp-16.2/GFP was reduced by 68.43% in the worms pretreated with 100 µg/mL GTE. N2 worms pretreated with 100 µg/mL GTE exhibited an increased survival rate of 48.31% after a lethal dose application of juglone. The results suggest that some green tea constituents are absorbed by the worms and play a substantial role to enhance oxidative stress resistance in C. elegans.
Keywords: LC/ESI-MS; catechins; antioxidants; DPPH; superoxide anion radical; hsp-16.2/GFP LC/ESI-MS; catechins; antioxidants; DPPH; superoxide anion radical; hsp-16.2/GFP
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Abbas, S.; Wink, M. Green Tea Extract Induces the Resistance of Caenorhabditis elegans against Oxidative Stress. Antioxidants 2014, 3, 129-143.

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