Next Article in Journal
Modeling Hybridization Kinetics of Gene Probes in a DNA Biochip Using FEMLAB
Previous Article in Journal
Avian and Mammalian Facilitative Glucose Transporters
Article Menu

Export Article

From the third issue of 2017, Microarrays has changed its name to High-Throughput.

Open AccessArticle
Microarrays 2017, 6(2), 8; doi:10.3390/microarrays6020008

Microarray Selection of Cooperative Peptides for Modulating Enzyme Activities

1
Department of Chemistry, Rutgers University-Camden, Camden, NJ 08102, USA
2
Center for Computational and Integrative Biology, Rutgers University-Camden, Camden, NJ 08102, USA
Academic Editor: Vehary Sakanyan
Received: 17 March 2017 / Revised: 21 April 2017 / Accepted: 24 April 2017 / Published: 26 April 2017
(This article belongs to the Special Issue Microarrays for Drug Discovery and Development)
View Full-Text   |   Download PDF [1836 KB, uploaded 27 April 2017]   |  

Abstract

Recently, peptide microarrays have been used to distinguish proteins, antibodies, viruses, and bacteria based on their binding to random sequence peptides. We reported on the use of peptide arrays to identify enzyme modulators that involve screening an array of 10,000 defined and addressable peptides on a microarray. Primary peptides were first selected to inhibit the enzyme at low μM concentrations. Then, new peptides were found to only bind strongly with the enzyme–inhibitor complex, but not the native enzyme. These new peptides served as secondary inhibitors that enhanced the inhibition of the enzyme together with the primary peptides. Without the primary peptides, the secondary effect peptides had little effect on the enzyme activity. Conversely, we also selected peptides that recovered the activities of inhibited enzyme–peptide complex. The selection of cooperative peptide pairs will provide a versatile toolkit for modulating enzyme functions, which may potentially be applied to drug discovery and biocatalysis. View Full-Text
Keywords: microarray; peptides; enzyme inhibition; β-galactosidase microarray; peptides; enzyme inhibition; β-galactosidase
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Fu, J. Microarray Selection of Cooperative Peptides for Modulating Enzyme Activities. Microarrays 2017, 6, 8.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Microarrays EISSN 2076-3905 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top