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From the third issue of 2017, Microarrays has changed its name to High-Throughput.

Open AccessArticle
Microarrays 2016, 5(2), 14; doi:10.3390/microarrays5020014

Retrospective Proteomic Analysis of Cellular Immune Responses and Protective Correlates of p24 Vaccination in an HIV Elite Controller Using Antibody Arrays

1
Department of Medicine, University of Sydney, Sydney 2000, Australia
2
Department of Cytogenetics, Children’s Hospital at Westmead, Sydney 2000, Australia
3
Australian Red Cross Blood Service, 17 O’Riordan Street, Alexandria NSW 2015 and School of Medical Sciences, (Faculty of Medicine) University of Sydney, Sydney 2000, Australia
*
Author to whom correspondence should be addressed.
Academic Editors: Carl A. K. Borrebaeck, Christer Wingren and Ulrika Andreasson
Received: 23 October 2015 / Revised: 14 January 2016 / Accepted: 25 January 2016 / Published: 2 June 2016
(This article belongs to the Special Issue Antibody Microarrays in Clinical Proteomics)
View Full-Text   |   Download PDF [1217 KB, uploaded 2 June 2016]   |  

Abstract

Background: HIV p24 is an extracellular HIV antigen involved in viral replication. Falling p24 antibody responses are associated with clinical disease progression and their preservation with non-progressive disease. Stimulation of p24 antibody production by immunization to delay progression was the basis of discontinued p24 vaccine. We studied a therapy-naive HIV+ man from Sydney, Australia, infected in 1988. He received the HIV-p24-virus like particle (VLP) vaccine in 1993, and continues to show vigorous p24 antigen responses (>4% p24-specific CD4+ T cells), coupled with undetectable plasma viremia. We defined immune-protective correlates of p24 vaccination at the proteomic level through parallel retrospective analysis of cellular immune responses to p24 antigen in CD4+ and CD8+ T cells and CD14+ monocytes at viremic and aviremic phases using antibody-array. We found statistically significant coordinated up-regulation by all three cell-types with high fold-changes in fractalkine, ITAC, IGFBP-2, and MIP-1α in the aviremic phase. TECK and TRAIL-R4 were down-regulated in the viremic phase and up-regulated in the aviremic phase. The up-regulation of fractalkine in all three cell-types coincided with protective effect, whereas the dysfunction in anti-apoptotic chemokines with the loss of immune function. This study highlights the fact that induction of HIV-1-specific helper cells together with coordinated cellular immune response (p < 0.001) might be important in immunotherapeutic interventions and HIV vaccine development. View Full-Text
Keywords: Antibody microarray; HIV; immune responses; chemokines; cytokines; Acquired immune deficiency syndrome (AIDS); p24 vaccine; monocyte; CD4+ T; CD8+ T cells Antibody microarray; HIV; immune responses; chemokines; cytokines; Acquired immune deficiency syndrome (AIDS); p24 vaccine; monocyte; CD4+ T; CD8+ T cells
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MDPI and ACS Style

Perera, S.S.; Wang, B.; Damian, A.; Dyer, W.; Zhou, L.; Conceicao, V.; Saksena, N.K. Retrospective Proteomic Analysis of Cellular Immune Responses and Protective Correlates of p24 Vaccination in an HIV Elite Controller Using Antibody Arrays. Microarrays 2016, 5, 14.

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