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From the third issue of 2017, Microarrays has changed its name to High-Throughput.

Open AccessCommunication
Microarrays 2015, 4(3), 370-388; doi:10.3390/microarrays4030370

SPOTing Acetyl-Lysine Dependent Interactions

1
Structural Genomics Consortium, Nuffield Department of Medicine, Oxford University, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UK,
2
Ludwig Institute for Cancer Research, Nuffield Department of Medicine, Oxford University, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UK
*
Author to whom correspondence should be addressed.
Academic Editors: Alexander Nesterov-Müller and Frank Breitling
Received: 26 June 2015 / Revised: 4 August 2015 / Accepted: 11 August 2015 / Published: 17 August 2015
(This article belongs to the Special Issue Peptide Microarrays)
View Full-Text   |   Download PDF [1625 KB, uploaded 17 August 2015]   |  

Abstract

Post translational modifications have been recognized as chemical signals that create docking sites for evolutionary conserved effector modules, allowing for signal integration within large networks of interactions. Lysine acetylation in particular has attracted attention as a regulatory modification, affecting chromatin structure and linking to transcriptional activation. Advances in peptide array technologies have facilitated the study of acetyl-lysine-containing linear motifs interacting with the evolutionary conserved bromodomain module, which specifically recognizes and binds to acetylated sequences in histones and other proteins. Here we summarize recent work employing SPOT peptide technology to identify acetyl-lysine dependent interactions and document the protocols adapted in our lab, as well as our efforts to characterize such bromodomain-histone interactions. Our results highlight the versatility of SPOT methods and establish an affordable tool for rapid access to potential protein/modified-peptide interactions involving lysine acetylation. View Full-Text
Keywords: lysine acetylation; bromodomain; epigenetic readout; SPOT assay; recognition motif lysine acetylation; bromodomain; epigenetic readout; SPOT assay; recognition motif
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Picaud, S.; Filippakopoulos, P. SPOTing Acetyl-Lysine Dependent Interactions. Microarrays 2015, 4, 370-388.

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