Next Article in Journal
A New Modified Histogram Matching Normalization for Time Series Microarray Analysis
Previous Article in Journal
Molecular Diagnostic Applications in Colorectal Cancer
Microarrays 2014, 3(3), 180-202; doi:10.3390/microarrays3030180
Review

Protein Microarrays with Novel Microfluidic Methods: Current Advances

†,*  and
Biomedical Diagnostics Institute, Dublin City University, Glasnevin, Dublin-9, Ireland Present Address: Faculty of mechanical Engineering, Technion-Israel Institute of Technology, Haifa-32000, Israel.
* Author to whom correspondence should be addressed.
Received: 30 April 2014 / Revised: 10 June 2014 / Accepted: 16 June 2014 / Published: 1 July 2014
View Full-Text   |   Download PDF [10133 KB, 2 July 2014; original version 1 July 2014]   |   Browse Figures

Abstract

Microfluidic-based micromosaic technology has allowed the pattering of recognition elements in restricted micrometer scale areas with high precision. This controlled patterning enabled the development of highly multiplexed arrays multiple analyte detection. This arraying technology was first introduced in the beginning of 2001 and holds tremendous potential to revolutionize microarray development and analyte detection. Later, several microfluidic methods were developed for microarray application. In this review we discuss these novel methods and approaches which leverage the property of microfluidic technologies to significantly improve various physical aspects of microarray technology, such as enhanced imprinting homogeneity, stability of the immobilized biomolecules, decreasing assay times, and reduction of the costs and of the bulky instrumentation.
Keywords: microarray; micromosaic; microfluidic networks; centrifugal arrays; microbeads microarray; micromosaic; microfluidic networks; centrifugal arrays; microbeads
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Export to BibTeX |
EndNote


MDPI and ACS Style

Dixit, C.K.; Aguirre, G.R. Protein Microarrays with Novel Microfluidic Methods: Current Advances. Microarrays 2014, 3, 180-202.

View more citation formats

Article Metrics

Comments

Citing Articles

[Return to top]
Microarrays EISSN 2076-3905 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert