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Brain Sci. 2013, 3(2), 877-907; doi:10.3390/brainsci3020877
Published: 3 June 2013
Abstract: Ischemic stroke exhibits a multiplicity of pathophysiological mechanisms. To address the diverse pathophysiological mechanisms observed in ischemic stroke investigators seek to find therapeutic strategies that are multifaceted in their action by either investigating multipotential compounds or by using a combination of compounds. Taurine, an endogenous amino acid, exhibits a plethora of physiological functions. It exhibits antioxidative properties, stabilizes membrane, functions as an osmoregulator, modulates ionic movements, reduces the level of pro-inflammators, regulates intracellular calcium concentration; all of which contributes to its neuroprotective effect. Data are accumulating that show the neuroprotective mechanisms of taurine against stroke pathophysiology. In this review, we describe the neuroprotective mechanisms employed by taurine against ischemic stroke and its use in clinical trial for ischemic stroke.
Stroke is one of the world’s leading causes of death and disability [1,2]. The World Health Organization (WHO) reported that 5.71 million people died of stroke in 2004 with an estimated acceleration to 7.8 million by 2030 . In keeping with this estimated trajectory of stroke, it was also reported that within the next decade there will be a 12% global rise in stroke morbidity . The two types of stroke are ischemic stroke and hemorrhagic stroke. Of the two approximately 85% are ischemic .
Ischemic stroke (cerebral ischemia) is due to a partial or complete reduction in blood flow to the brain. The ischemia may be global due to cardiac arrest or focal due to a blockage in a specific blood vessel. Brain regions most susceptible to damage are hippocampal CA1 and neocortical layers 3, 5, and 6 . Cerebral hypoxia (a reduction of cerebral oxygen) generally accompanies an ischemic insult but may also occur without the loss of blood flow as in the case of respiratory arrest, near-drowning or carbon monoxide poisoning . Insufficient oxygen and glucose supply in cerebral ischemia leads to unsustainable cellular homeostasis which initiates cell injury. Cellular injury progresses as a result of excitotoxicity, ionic imbalance, oxidative and nitrosative stresses, endoplasmic reticulum (ER) stress and mitochondrial disturbances, ultimately resulting in programmed cell death and necrosis . Pathologically the ischemic infarct is observed as a central core, a region where cells undergo anoxic depolarization and never repolarize. Cells in the core eventually become necrotic  immediately surrounding the core (perifocal region) is the ischemic penumbra, a region where cells receive some perfusion via collateral circulation and may repolarize but they are still highly vulnerable to injury . Cells in the penumbra are subject to apoptosis but may be rescued by neuroprotective measures . If the ischemic process is not arrested the ischemic core will recruit the perifocal penumbra by a process called “spreading depression”; which is unarrested massive depolarization [11,12]. Manifestation of cerebral ischemia involves neurological deficit in cognition, motor and sensory functions, the severity of which reflects the location and size of the damaged area.
Due to the multiple pathophysiological mechanisms observed in ischemic stroke/cerebral ischemia, current treatments remain mostly ineffective apart from thrombolytic therapy which uses thrombolytic recombinant tissue plasminogen activators (rt-PA)  such as alteplase . This therapy allows only a 3–4.5 h window for effective treatment. It therefore becomes critical to develop other compounds that are multipotential in addressing the diverse pathological mechanisms in ischemic stroke/cerebral ischemia.
Taurine (2-amino-ethanesulfonic acid) is a sulphur containing, free amino acid, (Figure 1A) that is abundantly found in mammals . Unlike other amino acids the presence of a sulphur group instead of a carboxyl group prevents it from being incorporated into proteins. It is mostly found in excitable tissues such as the brain, retina, cardiac muscle and skeletal muscle [16,17,18,19,20,21,22] and it is synthesized by methionine and cysteine metabolism with cysteine sulphinic acid decarboxylase (CSAD) being the rate-limiting enzyme [23,24] (Figure 1B). Apart from being synthesized endogenously, taurine may also be obtained from meat, dairy products, poultry, fish and shellfish [25,26]. While it was first discovered as a component of ox (Bos tauru; from which its name is derived) bile in 1827, it had taken over a century before insights into its physiological functions were made. Early concepts of its physiological functions were provided in a study by Curtis and Watkins . They demonstrated that taurine could be a neurotransmitter which was later supported by Davison and Kaczmarck . There is growing evidence of its physiological importance. Hayes and colleagues  reported that cats fed a taurine deficient diet developed central retinal degeneration. This report by Hayes and colleagues emphasized that taurine was essential in species that are unable to synthesize it, such as cats. The importance of taurine in retinal function was also supported by several authors [30,31,32]. Taurine is also a key player in: cardiovascular and muscular skeletal functions [33,34,35], regulating the release of pancreatic insulin  and renal function . Interestingly, taurine’s role in brain development involves the differentiation and migration of cerebellar, pyramidal and visual cortical cells in cats and monkeys [37,38,39,40] as well as playing a role in both embryonic and adult neurogenesis [40,41]. These lines of evidence highlight a few of its physiological functions, for review [19,42,43]. Taurine also exhibits its potential to be effective against many diseases as observed in animal models of Type 1 and Type 2 diabetes [44,45,46], atherosclerosis  and neurological disorders such as Alzheimer’s, Parkinson’s and Huntington’s diseases . Its depletion has also been reported in cardiomyopathy [49,50].
Physiological Function of Taurine in Central Nervous System
Taurine is able to cross the blood-brain barrier [51,52] and displays a plethora of functions in the central nervous system (CNS) [42,53,54]. In the CNS it plays a role in: neuromodulation [55,56,57], osmoregulation [33,58], the maintenance of calcium homeostasis [59,60,61,62,63], membrane stabilization , anti-oxidation [65,66], anti-inflammation [67,68] and neuroprotection [59,69,70,71,72,73] and is also seen as a trophic factor during CNS development [40,74]. Its neuroprotective effect is observed against l-glutamate induced excitotoxicity whereby it counteracts the glutamate-induced increase of intracellular calcium through L-, P/Q-, N-type voltage-gated calcium channels (VGCCs) and the N-methyl-d-aspartate (NMDA) receptor, thus preventing glutamate-induce membrane depolarization [59,69].
Although taurine is not definitively classified as a neurotransmitter it fulfills most of the necessary criteria: it is associated with synaptic membrane structures  and both taurine and its synthesizing enzyme CSAD, are co-localized in presynaptic neuronal terminals [23,76,77]; stimulated-taurine release is both calcium dependent (at a potassium concentration up to 40mM) and calcium independent (at a potassium concentration greater than 40mM) ; taurine is taken up into the cell by a sodium-dependent taurine transporter (TauT) [79,80,81]. It modulates neurotransmission by eliciting inhibitory neuronal transmission through GABAA receptors, glycine receptors and putative taurine receptors [82,83,84,85]. The only criterion that has not been met for it to be fully classified as a neurotransmitter is the cloning of a taurine specific receptor. Previously we identified a putative taurine receptor ; our study and the later investigation by Frosini et al. 2003  demonstrated that the receptor is neither activated nor antagonized by structurally similar amino acids such as glutamate, gamma-amino butyric acid (GABA) and glycine.
The fundamental pathophysiological mechanisms involved in ischemic stroke are glutamate excitotoxicity, calcium imbalance and oxidative stress which individually or collectively results in cell death. Therefore taurine’s role as an inducer of inhibitory neurotransmission, an anti-oxidant, neuromodulator, regulator of calcium homeostasis and neuroprotector, potentially makes it an ideal therapeutic agent for ischemic stroke. This review will focus on previous and current studies of taurine’s neuroprotective effect on ischemic stroke with an insight to the underlying mechanisms employed against the pathophysiology of ischemic stroke and the possibility of its use in clinical trials for ischemic stroke patients.
2. Neurochemical Mechanisms of Ischemic Stroke
Three major mechanisms attribute to brain damage in ischemic stroke; glutamate excitotoxicity which leads to an increase of intracellular cytosolic calcium, acidosis and increased production of free radicals. Within 10–20 s of the insult there is a loss of consciousness and loss of neuronal electrical activity within the ischemic area [88,89]. This initial 20 s is followed by the failure of energy-dependent pumps, such as the Na+/K+-ATPase and Ca2+-ATPase pump, impairment of the energetics required to maintain ionic gradients, and a resulting imbalance of ion homeostasis . Increased influx of Na+ and reduced efflux of K+ induce membrane depolarization of neurons and glia, is followed by the resulting influx of Ca2+ through VGCC’s  and release of the excitatory amino acid; glutamate . Both in vitro and in vivo studies have shown a massive release of glutamate during ischemic stroke [93,94,95,96,97,98]. Interestingly, glutamate release during ischemic stroke can also be Ca2+-independent, distinct from exocytosis, by passing through volume-regulated anion channels (VRAC) [99,100,101]. Another source that accounts for the increased accumulation of extracellular glutamate is the reversal of the glutamate transporter (GLT-1) which occurs due to increases in intracellular Na+ and extracellular K+ [102,103].
Contributions to cytosolic Ca2+ overload in the ischemic cell arise from many processes. Initial increases occur through the action of VGCCs and the reverse mode of the Na+/Ca2+ exchanger . Excessive extracellular glutamate hyperactivates ionotropic and metabotropic glutamate receptors, NMDA, AMPA/Kainate and mGluRs respectively . Hyperactivation of these receptors results in augmented Ca2+ permeability of the receptors especially through the NMDA receptors. Normally AMPA/Kainate receptors are not permeable to Ca2+ but ischemia activates a population of AMPA receptors that are Ca2+ permeable . Activation of mGluRs results in Ca2+ release from calcium stores, such as the endoplasmic reticulum (ER) via the binding of inositol, 1,4,5-triphosphate (IP3) to its receptor (IP3R). The ryanodine receptor (RyR), located on the ER membrane will also release Ca2+ from the ER via a calcium-induced-calcium-release mechanism [107,108]. An imbalance in ER calcium homeostasis propagates ER stress and resultant apoptosis . Extracellular and intracellular acidosis (fall from pH 7.3 to 6.2) develop concurrently with the imbalance of calcium homeostasis in response to the production of lactic acid during anaerobic metabolism. Acidosis can specifically elicit early necrosis and delay apoptosis in the ischemic cell . Although acidosis may be a direct cause of cell damage/death, it also augments increased cytosolic calcium through acid-sensing ion channels (ASICs) .
Excessive cytosolic calcium initiates cellular events, by activating catabolic enzymes such as proteases , phospholipases and endonucleases , which initiate the development of cellular injury and cell death. For example increases in [Ca2+]i activate phospholipase A2 (PLA2) which acts on membrane phospholipids, altering membrane structure and rendering it more permeable . One important physiological function of the mitochondria is to sequester cytosolic calcium. The mitochondrion becomes dysfunctional in brain ischemia due to excessive intra-mitochondrial calcium, eliciting excessive production of free radicals/reactive oxygen species (ROS) such as superoxide, hydrogen peroxide and nitric oxide [115,116,117]. The production of ROS is augmented in the reperfusion phase of brain ischemia due to the reintroduction of oxygen to the injured site. Unscavenged excessive ROS causes protein- and lipid-oxidation, interfering with membrane structure and causing DNA damage which inevitably leads to necrotic and apoptotic cell death [118,119]. The pathogenesis of ischemic stroke also involves a deficiency of inhibitory GABAergic mechanisms due to the activation of the mGlu1 receptor located on GABAergic neurons. Extracellular glutamate activation of presynaptic mGlu1 receptors on GABAergic interneuron suppress the release of GABA  resulting in a subsequent imbalance between the excitatory and inhibitory neurotransmitter systems.
3. Anti-Ischemic Stroke Mechanisms of Taurine
A 90% reduction in stroke incidence was observed in a genetic rat model of stroke (SHRSP: Stroke-prone spontaneously hypertensive rat), fed a fish diet rich in taurine . We have demonstrated that taurine neurons in rat hippocampus are relatively inert to cerebral ischemia . These observations called attention to the prospect that the prevention and/or amelioration of ischemia in these reports could be that the onset of an ischemic insult triggered the protective mechanism(s) of taurine, thereby protecting the brain from the ischemic on slate . In a rabbit model of transient focal ischemia (trans-orbital three vessel occlusion), using in vivo microdialysis, Matsumoto and colleagues measured the evoked release of excitatory (glutamate and aspartate) and inhibitory (GABA and taurine) amino acids into the extracellular space of the cerebral cortex. The study showed a concomitant increase of extracellular glutamate, aspartate and GABA as well as taurine . The reduction of glutamate and taurine immunolabeling of pyramidal cell bodies provide an anatomical support to Matsumoto’s study of an efflux of these amino acids from neurons . Several lines of evidence from both in vitro and in vivo experiments have provided additional support for the increased release of taurine in cerebral ischemia [126,127,128,129,130]. Recently it has been shown that the ischemic-induced release of taurine is glutamate receptor-mediated . Saransaari and Oja demonstrated that brain stem slices of the adult mouse released preloaded [H3] taurine under ischemic conditions. The release was enhanced in the presence of both ionotropic (NMDA, AMPA and kainate) and metabotropic (mGluR I and mGluR III) glutamate receptors agonists while it was reduced in the presence of their antagonists. This indicates that in ischemia, the activation of glutamate receptors will enhance the consequential release of taurine. This increase in extracellular taurine may serve as compensatory protective response which aims at counteracting glutamate excitotoxicity.
3.1. Taurine’s Neuroprotective Effect against Glutamate Excitotoxicity
We and other investigators have established that taurine has a protective effect in cultured neurons against glutamate-induced excitotoxicity [59,61,69,132,133,134,135,136,137]. Such observations from these studies have shown that taurine’s neuroprotective mechanism acts through the maintenance of intracellular calcium homeostasis via the inhibition of the Na+/Ca2+ exchanger reverse mode , inhibition of L-, P/Q-, N-type voltage-gated calcium channels , prevention of Ca2+ influx through NMDA receptor calcium channels , inhibition of calcium release from the endoplasmic reticulum , and the maintenance of intra-mitochondrial calcium homeostasis . Although the reported neuroprotective mechanisms of taurine were observed in glutamate-induce neuronal damage using a cell culture system there is also evidence that taurine’s neuroprotective effects are observed in both in vitro and in vivo models of ischemic stroke. Schurr and colleagues  reported that hypoxia-induced hippocampal brain slices pre-treated with taurine improves the synaptic function of hippocampal neurons in a dose-dependent manner by attenuating Ca2+ movement across the membrane. Ricci and colleagues reported that taurine protects rat brain cortical slices against oxygen/glucose deprivation-reoxygenation . In a chemically-induced (2,4-dinitrophenol) hypoxic model of rat hippocampal neuronal cultures, intracellular calcium was inhibited by taurine (3mM) . Taurine also protects against glutamate excitotoxicity via the activation of GABAA and strychnine-sensitive glycine receptors. This was shown in a rat model of transient focal ischemia, whereby the middle cerebral artery (MCA) was occluded for 2 h . In this study taurine was given pre- and post-ischemia and in several paradigms taurine significantly reduced the neurological deficit score (a score given to indicate the severity of ischemia based on the animal’s behavior), infarct volume and brain water content compared to control animals. It was also noted that this effect of taurine was only partially reduced with either the GABAA antagonist (bicuculline) or the glycine antagonist (strychnine) but completely abolished with the co-application of both antagonists, indicating that taurine’s effect is through both receptors. These studies on hypoxic-ischemic stroke models add credence to taurine’s neuroprotective mechanism of eliciting inhibitory neurotransmission, thereby attenuating the deplorizing-evoked component of glutamate excitotoxicity , maintaining calcium homeostasis as mentioned above. The reduction of intracellular Ca2+ overload has resulting anti-necrotic and anti-apoptotic effects because of the consequential influence on the regulation of activities of calpains and caspases and on mitochondrial function.
3.2. Taurine’s Neuroprotective Effect on Mitochondrial Dysfunction, Calpain and Caspase Activities
Excessive intracellular Ca2+ results in mitochondrial calcium overload and mitochondrial dysfunction . Dysfunctional mitochondrial related-events involves; the collapse of the mitochondrial membrane potential (Δψm), an opened mitochondrial permeability transition pore (MPTP: a pore that spans both the inner and outer mitochondrial membrane), the subsequent release of pro-apoptotic proteins, such as cytochrome C [146,147,148] and the uncoupling of oxidative phosphorylation with a consequential decrease in adenosine tri-phosphate (ATP) production . Interestingly, taurine is found in high concentration in the mitochondria [150,152] and evidence has been presented showing taurine to be a buffering agent for intra-mitochondrial calcium level [62,153] as well as buffering the pH of the mitochondrial matrix . The buffering action of taurine in the mitochondria has proven to be a protective mechanism in ischemic stroke pathology . El Idrissi demonstrated that taurine was able to maintain intra-mitochondrial calcium homeostasis in glutamate-induced excitotoxicity in cerebellar granule cells exposed to increase [Ca2+]i . Using a rat retinal ganglion cell line exposed to hypoxia for 24 hrs, Chen and colleagues  demonstrated that taurine prevented mitochondrial dysfunction. The investigators showed a reduction in the extent to which the MPTP was open in taurine-treated cells. The decrease in the Δψm was more significant in non-taurine treated cells than the treated cells and that there was an overall increase in ATP production, again in the taurine-treated cells . Although Chen and colleagues did not report on the precise mechanism of taurine’s protective action against mitochondrial dysfunction, it can be postulated this is due to taurine’s capacity to either buffer intra-mitochondrial calcium  and/or buffer the alkaline pH of the mitochondria’s matrix [154,156]. Intriguingly, as part of a post-transcriptional modification, taurine is incorporated into uridine of the mitochondria’s transfer RNALue(UUR) [mt tRNALue(UUR)] at the wobble anticodon position, thus modifying uridine into 5-taurinomethyluridine . A mutated mt tRNALue(UUR) hinders the incorporation of taurine, resulting in a defective mt tRNALue(UUR) that is unable to recognize it cognate codon, UUG . This defect causes mitochondrial dysfunction which appears as stroke-like episodes (one of the conditions associated with MELAS: mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes) [158,159]. Rikimaru and colleagues using a culture system of MELAS patient-derived pathogenic cells reported that a high concentration of taurine (40 mM for 4 day exposure) was able to reverse the mitochondrial dysfunction observed in these patients. In their study, taurine increase the oxygen consumption rate, increased the Δψm in a time-dependent and dose-dependent (0 mM, 20 mM and 60 mM) manner and reduced oxidative stress . The study also reported that the MRI of MELAS patients, treated with taurine, showed a reduction in the spread of the ischemic infarct to different brain region. These protective mechanisms of taurine in these patients were evident as an amelioration of stroke-like episodes.
The activation of calpains and caspases results in apoptotic and necrotic cell death. There is increasing evidence that both calpains and caspases play a major role in ischemia-mediated cell death [161,162,163,164,165,166,167]. Calpains (µ-calpain and m-calpain) are members of the cysteine protease family, are activated by Ca2+ (micromolar [Ca2+]i and millimolar [Ca2+]i, respectively) and are endogenously inhibited by calpastatin . Interestingly members of the Bcl-2 family are calpain substrates [168,169]; for example B-cell lymphoma-2 (Bcl-2) and Bcl-xL are both anti-apoptotic molecules but when cleaved by calpain they are converted to pro-apoptotic molecules . On the other hand calpain cleavage of Bcl-2 associated protein X (Bax), a pro-apoptotic molecule, results in increased levels of its active form (a 18 kDa Bax) which possesses more potent cytotoxicity than uncleaved Bax . A decreased ratio of Bcl-2or Bcl-xL to Bax favors cell death . Gil-Parrado reported decreased formation of Bcl-2 and Bax heterodimer formation after calpain cleavage . Caspases, also members of the cysteine protease family, are key executioners of apoptosis, with caspase-3 seen as the final killer in the apoptotic cascade . The activation of caspase-3 can be mitochondrion-mediated due to the release of cytochrome c through opened mitochondrion permeable transition pores (MPTPs). Once in the cytosol, cytochrome c becomes associated with apoptotic protease activity factor-1 (Apaf-1), forming an apoptosome (a large complex molecule). Caspase-9, an initiator caspase becomes activated via protein-protein interaction with the apoptosome, which then activates the downstream effector caspase, caspase-3 . It has been shown that taurine attenuates the amount of caspase-9 associated with Apaf-1 in ischemia . This study provided evidence that taurine is protective at the Apaf-1/caspase-9 step of the mitochondrial-mediated apoptotic cascade. The translocation of cytosolic Bax to the mitochondrial membrane affects the MPTP; by forming a homodimer, Bax increases the opening of the MPTP. We and others  have shown that taurine attenuates mitochondrion-mediated-death pathways. Using a glutamate-induced neuronal damage culture system we have recently observed that taurine is able to shift the ratio of Bcl-2:Bax in favor of cell survival and that it also inhibits the glutamate-induced activation of calpain, resulting in an increased formation of Bcl-2/Bax heterodimers . The increase in the formation of Bcl-2/Bax heterodimers cause a decrease in mitochondrial release of cytochrome c and an inhibition of the caspase-apoptotic cascade . Protection against necrosis and apoptosis by taurine was also observed in experimental stoke models. In models of focal cerebral ischemia, taurine was reported to inhibit the ischemia-induced activation of m-calpain (no significant effect was observed on µ-calpain) in a dose-dependent manner by enhancing the expression and activity of calpastatin. Also in this model, Bax and caspase-3 were downregulated while Bcl2-xL was upregulated, which results in the attenuation of mitochondrial cytochrome c release and the consequential reduction of the mitochondrial-mediated apoptotic and necrotic cell death in the ischemic penumbra and core [177,178]. Taranukhin and colleagues also reported that taurine reduces ischemia-induced caspase-8 and caspase-9 expression (two upstream activators of caspase-3) in the paraventricular nucleus (PVN) and supraoptic nucleus (SON) of the rat’s hypothalamus .
3.3. Taurine’s Neuroprotective Effect against Endoplasmic Reticulum Stress
In addition to the cell death mediated by the mitochondrion, increasing evidence points to ER stress as a critical player in hypoxic-ischemic cell death [109,180,181]. The ER is an essential sub-cellular organelle responsible for calcium storage and signaling, calcium-dependent processes such as the folding and processing of synthesized proteins and lipid biosynthesis [182,183,184]. Ischemic stroke induced ER stress, resulting in the impairment of ER protein folding . An accumulation of unfolded/misfolded proteins activates the unfolded protein response (UPR)  mediated by ER transmembrane stress sensors, inositol-requiring kinase 1 (IRE1), double-stranded RNA-activated protein kinase 1 (PKR)-like endoplasmic reticulum kinase (PERK), and activating transcription factor 6 (ATF 6) . Each stress sensor activates corresponding intracellular pathways (the IRE1-, PERK- and ATF6-pathways; Figure 2) that in turn mediate the up-regulation of the transcription factor C/EBP homologous protein (CHOP) also known as growth arrest and DNA damage inducible protein 153 (GADD 153) . The UPR also activates caspase-12, an essential player in ER stress-mediated apoptosis . In an in vitro model of hypoxia/reoxygenation, (0.3%, oxygen for 24 h, followed by reoxygenation at 21% oxygen for 24–48 h) we observed the neuroprotective effect of taurine against ER stress-mediated apoptosis . In this study, taurine significantly increased the cell viability of the primary neuronal culture. The neuroprotective effect of taurine was dose-dependent, inhibiting the expression of CHOP and of caspase-12. The ratio of cleaved ATF6 to ATF6 declined by 50% in neurons treated with taurine relative to neurons exposed to hypoxia/reoxygenation alone, thereby inhibiting the ATF6-pathway. We also observed that taurine dramatically reduced the expression of p-IRE1 (the activated form of IRE1) in the IRE1 pathway but had no effect on the PERK pathway . In a later study, using experimental stroke model of middle cerebral artery occlusion, (2 h ischemia followed by 4 days reperfusion) we observed that taurine attenuated infarct volume in 2 mm brain slices 6 mm from the frontal pole. Taurine’s neuroprotective effect on ER stress molecules was similar to our in vitro study; a reduction in the expression of CHOP, caspase-12, p-IRE1 and ATF6 . We also observed that GRP78, another ER stress marker was reduced by taurine in this later study . These studies provide convincing evidence that taurine is able to protect the ischemic brain against ER stress and subsequently ER stress mediated apoptosis, since CHOP; a transcription factor that upregulates the transcription of pro-apoptotic Bim (Bcl2 interacting mediator of cell death), and PUMA (p53 upregulated modulator of apoptosis) , while downregulating the transcription of anti-apoptotic Bcl2 , was downregulated by taurine.
3.4. Taurine’s Neuroprotective Effect against Oxidative Stress, Inflammation and Edema
Other functions of taurine, such as anti-oxidative, anti-inflammatory or osmoregulatory could also contribute to its neuroprotective mechanism [19,72,193] against ischemic stroke. In the presence of high mitochondrial calcium, the physiological production of reactive oxygen species [(ROS: such as superoxide anion (O2−), hydrogen peroxide (H2O2) and hydroxyl radical (·OH)] and nitric oxide NO, becomes pathological due to an imbalance of their production versus their degradation . This pathological condition is commonly referred to as oxidative stress and it is observed in ischemic stroke . The excessive intra-mitochondrial calcium [Ca]m is able to generate excessive ROS by one or all of such methods. Firstly, [Ca]m stimulates the tricarboxylic Acid (TCA) cycle, which enhances electron flow into the electron transport respiratory chain, increasing the mitochondrion’s work and simultaneously increase the respiratory chain electron leakage to the acceptor O2, generating ROS [196,197]. Secondly, [Ca]m disrupts the electron transport respiratory chain by either affecting conformational changes  in the respiratory complexes, exemplified by changes in complex lV (Wikstrom and Saari  or by activating the Ca2+-dependent production of nitric oxide (via nitric oxide synthase), which inhibits complex I directly  and complex III in conjunction with calcium  of the respiratory chain. The release of cytochrome c from the mitochondria inhibits complex lV . This bottle neck in the respiratory chain diverts the flow of electrons from the chain to O2. Thirdly, [Ca]m inactivates ROS scavengers, such as glutathione peroxidase (GPx)  resulting in a decrease in antioxidant capacity in the mitochondria. It is to be noted as well that the loss of GPx via an opened MPTP further reduces mitochondrial GPx . Recci and colleagues  attributed the loss of mitochondrial respiratory chain integrity to a decline in the synthesis of the encoded proteins in the respiratory complexes.
Several lines of evidence have shown taurine to be protective against oxidative injury [65,206,207,208,209,210]. Taurine reduces ROS not by directly scavenging ROS  but instead by potentiating or rescuing endogenous anti-oxidants, as reported by many of these studies. Interestingly, Jong and colleagues reported that taurine’s anti-oxidative effect is due to the maintenance of the mitochondrial respiratory chain integrity by taurine . By using β-alanine, an inhibitor of taurine-linked reactions  they showed a reduction in complex l and complex III activity of the mitochondrial respiratory chain with a simultaneous reduction in oxygen consumption and an increase in mitochondrial oxidative stress (enhanced superoxide production, oxidation of glutathione and inactivation of aconitase, an oxidant sensitive enzyme). The reduction in complex 1 activity correlates with a reduction in the synthesis of mitochondrial proteins, ND5 and ND6; proteins that are apart of complex 1 protein assembly and are also encoded by taurine-conjugated mt tRNALeu(UUR). The encoding function of mt tRNALeu(UUR) was impaired by the taurine competitor, β-alanine. The reductions of oxidants reduce cellular damage such as membrane lipid peroxidation.
An inflammatory reaction occurs in response to brain ischemic stroke due to the infiltration of neutrophils, macrophages, activated microglia and inflammatory mediators such as various cytokines, adhesion molecules, and chemokines . The transcription factor, nuclear factor-κB (NF-κB), enhances the production of inflammatory mediators by transcribing inflammatory genes. NF-κB’s action is potentiated by poly-ADP-ribose polymerase (PARP) which is reported to act as its co-activator [215,216]. Inflammation was reduced by taurine (50 mg/kg b.wt.) in a rat model of transient focal ischemia . These investigators observed that the up-regulation of PARP and NF-κB in the ischemic core and penumbra was reversed by taurine and that the levels of the inflammatory cytokines, tumor necrosis factor-α and interleukin-1β, were significantly reduced . Edema is one of the resulting conditions after a brain ischemic insult. This is caused by an accumulation of intracellular Na+ and Cl− which instigates osmotic water influx . Swelling then potentiates taurine’s release; initially release is exocytosis and Ca2+-dependent, subsequently through the reverse mode of the Na+/Cl−-dependent TauT [219,220], as the ischemic insult prolong, release is through volume-sensitive chloride channels [221,222,223,224], and finally by diffusing across a permeabilized plasma membrane . This regulates the cell’s volume, preventing cell death by necrotic swelling. It was shown that taurine significantly reduced cell swelling in rat brain cortical slices after exposure to oxygen-glucose deprivation and reoxygenation .
In spite of the plethora of convincing demonstrations of the neuroprotective effect of taurine in ischemic stroke there are conflicting reports showing the failure of taurine to protect against this type of brain insult. Shuaib, reported that taurine (100 mg/kg, i.p.) did not statistically reduce infarct volume . This contradictory report could be due to differences in experimental conditions, animal model, and route of administration or more interestingly, by a dose-dependent biphasic response of taurine in which a low concentration of taurine (1 mM) elicits hyperpolarization but at a higher concentration (10 mM), hyperpolarization is followed by slow depolarization . Taurine’s biphasic effect in an ischemic insult was clearly seen in an in vivo model of hypoxia-induced convulsion where taurine suppresses convulsion in a dose-dependent manner but at a high dose of 100 mg/kg no protection was observed . We have also demonstrated the biphasic effect of taurine in excitatory amino acid-induced neurotoxicity in primary neuronal cultures . The biphasic response of taurine cautions investigators about the dose to be administered in their experiments which should be empirically determined, especially when using different experimental models.
4. Clinical Trial
There is a growing body of preclinical data that demonstrates taurine’s neuroprotective effect in cerebral ischemic stroke. It is a lipophobic amino acid and while the BBB prevents significant amount of exogenous taurine from entering the brain, in cerebral ischemia the BBB is damaged  which allows free access of exogenous taurine to injured neurons and glia. Inspite of accumulating data there seems to be a paucity of studies on taurine in clinical trials for stroke. Much of the human trials with taurine involving ischemia-reperfusion injury were reported for cardiovascular diseases [229,230,231,232,233]. For instance, Azuma and colleagues orally administered taurine (3 mg/day) to 17 patients with congestive heart failure (CHF: observed as reduced left ventricular function), a secondary condition of heart ischemia, for 6 weeks. They reported that there was a significant improvement in systolic left ventricular function in the taurine-treated group compared to the group treated with coenzyme Q10 . Similar effect of taurine to improve congestive heart failure was observed in an earlier clinical study by Azuma and colleagues . In this study the group given taurine orally showed significant improvement in CHF over the group given placebo. No adverse effect from taurine was reported in either study. Interestingly other investigators provided evidence that an intake of dietary taurine improved ischemic heart disease. Yamori and colleagues performed an epidemiological study (in 19 centers of 14 countries, including both sexes) of taurine’s effect on ischemic heart disease. They used 24 h urinary (24-U) taurine excretion as a biological marker for dietary intake of taurine. They reported that there was a significant inverse correlation with the 24-U taurine excretion and ischemic heart disease in both sexes . Later Yamori and colleague conducted another but similar epidemiology study using a larger population . The findings from the later study confirmed that of their former study. We postulate that the high intake of dietary taurine could be protecting the heart cells from further death due to ischemia in these epidemiology studies by Yamori.
Recently Rikimaru and colleagues reported that the addition of taurine to the culture media of MELAS patient-derived cells ameliorated reduced oxygen consumption, improves the mitochondrial membrane potential and reduced oxidative stress . In the same study Rikimaru and colleagues reported that taurine protected the brains of two MELAS patients from the spread of stroke-like lesions, revealed by MRI. Taurine neuroprotective effects in these patients were manifested in an amelioration and eventually complete cessation of stroke-like episodes. In this small clinical study a daily dose of taurine (0.25 g/kg/day) was administered for over a period of nine years but protection was observed from the beginning of the study (year one) as seen from MRI of the brain and the alleviation of symptomatic stroke-like episodes . Apart from this small study there seems to be no other clinical study on taurine in stroke patients. The deficiency of taurine usage in cerebral ischemic stroke trials could be due to insufficient preclinical experiments that have rigorously validated the Stroke Therapy Academic Industry Roundtable (STAIR) criteria for neuroprotective stroke agents. An updated version of the STAIR criteria includes: (1) Identification of the minimum effective and maximum tolerable dosage. (2) Identification of a therapeutic window; suggested usage of mismatched between perfusion-weighted MRI (monitors blood supply of the tissue) and diffusion-weighted MRI (measures tissue damage) of the penumbra would be a useful aid for this identification. (3) Both histological and behavioral measurements of experimental studies should be endpoint assessments and that studies should be performed over a 2–3 week after stroke on-set, to demonstrate sustainability of the tested compound. (4) Physiological parameters such as blood pressure, body temperature, blood gases and glucose should be routinely monitored. A Doppler Flow apparatus or perfusion MRI should be used to monitor decreased blood flow and reperfusion in temporary ischemic models. (5) Data obtained in one laboratory should be replicable in at least one other independent laboratory. (6) Efficacy study should be done on animals of both sexes and of all ages, interaction between tested compound and medication commonly used by stroke patients should be performed, serum markers of tissue injury similar to those obtainable in human trial should be used and studies should include more than one type of animal species. In addition, studies in animals with comorbidities such as hypertension, diabetes and hypercholesterolemia should be carried out if this is the targeted population for human trial . For a successful translation to clinical trials more preclinical experiments need to be performed with the STAIR’s criteria in mind. Similarly, more clinical studies need to be done on the changes of taurine levels in stroke patients which would provide insight as to whether changes caused by ischemic stroke are the same in animal and patients . This would provide a more credible extrapolation from experimental studies to patients.
Another concern in translating taurine from the bench to the bedside is the potential of any adverse side effects. Taurine is a naturally occurring amino acid of the body and should therefore exhibit minimal, if any, adverse side effects. Toxicology studies have not reported any genotoxic, carcinogenic or teratogenic effects of taurine [236,237,238,239]. An area of controversy on the safety issue of taurine usage is in the maximum tolerable dosage of taurine/No Observed Adverse Effect Level (NOAEL). Furukawa and colleagues reported that a 13 week intravenous taurine treatment resulted in water consumption (1000 and 2000 mg/kg b.wt/day taurine intake) and haemosiderin (a denatured ferritin complex which poorly provide available iron when needed) deposition in the lungs (2000 mg/kg b.wt./day taurine intake) . The authors concluded that the NOAEL was 500 mg/kg b.wt./day . While Cantafora and others reported that taurine (462 mg/kg b.wt./day) administered in drinking water of guinea pigs for two weeks resulted in fatty infiltration of the liver . Recently the European Food Safety Authority (EFSA) shared their opinion that 1000 mg/kg b.wt./day was the NOAEL for taurine in energy drinks . Attention should be drawn to the fact that this dose (1000 mg/kg b.wt./day) of taurine was in energy drinks which consisted of other active compound and therefore any potential adverse effect at this dose of taurine maybe camouflaged by the other ingredients. This dosage (1000 mg/kg b.wt.) may also not be neuroprotective in cerebral ischemia due to taurine’s biphasic mechanistic mode of action (commented on previously) observed in ischemic preclinical studies [136,225,226,227].
5. Concluding Remarks
Taurine is synthesized within brain cells and exhibits a plethora of physiological functions . It has a multiple of neuroprotective mechanisms in the CNS such as: regulating cellular osmolarity [243,244], an anti-oxidant [65,66], neuromodulator of GABAergic transmission [85,245,246], maintaining calcium homeostasis [59,60,61,62,63], inhibiting glutamate excitotoxicity [59,69,133], attenuating endoplasmic reticulum stress [73,190,247], modulating the mitochondrial pore permeability  downregulating a range of pro-apoptotic proteins while upregulating anti-apoptotic proteins [70,176,179,248] and downregulating inflammatory mediators . In ischemic stroke, a pathological brain condition, taurine is released in the extracellular space resulting in a decrease in the concentration of intracellular taurine. The decrease in intracellular: extracellular taurine ratio attenuates the protective role of taurine and could potentiate neuronal damage during ischemia. The administration of exogenous taurine protects the neuropile in ischemic stroke, evident from numerous experimental reports [23,63,65,69,70,73,140,141,142,144,154,155,156,160,176,179,190,206,207,208,209,210,212,217]. Exogenous taurine is able to elicit its neuroprotective mechanisms both at the intracellular and the extracellular level. Taurine administered subcutaneously (s.c.), intravenously (i.v.) or intraperitonely (i.p.) is able to cross the BBB in ischemia . Once it crosses the BBB it may be transported into cells via the taurine transporter (TauT) [249,250], where it is able to mediate its protective mechanisms in key subcellular organelles suchthe mitochondria and the endoplasmic rectiulum. In the mitochondrion, taurine buffers [Ca]m  and the mitochondrial pH [154,156], two important parameters that maintain the integrity of the mitochondrial membrane potential (Δψm), preventing mitochondrial-mediated apoptosis via the activation of caspases [70,155,178]. Within the mitochondrion, taurine may also attenuates excessive ROS generated in ischemic stroke [65,212]. Taurine protects the endoplasmic reticulum from being stress, evidential by the reduction of ER stress markers such as CHOP and caspase-12 in taurine-treated experimental models [73,190,247].
Several lines of evidences have reported that extracellular taurine modulates inhibitory neurotransmission via GABAA and glycine receptors [83,85,143]. The activation of these inhibitory receptors attenuates the influx of calcium, protecting the ischemic brain against glutamate-mediated apoptosis . We have also demonstrated that taurine counteracts the glutamate-induced increase of intracellular calcium through L-, P/Q-, N-type voltage-gated calcium channels (VGCCs) and the N-methyl-d-aspartate (NMDA) receptor, thus preventing glutamate induced membrane depolarization [59,69]. Although there is no cloned taurine receptor, several studies have provided strong evidence of the existence of a specific taurine receptor [86,251,252,253,254]. In our previous studies , we demonstrated that the receptor is neither activated nor antagonized by structurally similar amino acids such as glutamate, GABA and glycine. These observations were later supported by Frosini and colleagues . There is also a strong possibility of there being two types of taurine receptor; an ionotropic taurine receptor  and a metabotropic taurine receptor . Other researchers have also demonstrated the existence of distinct types of taurine receptor . We propose that taurine’s neuroprotective effect against glutamate-induced apoptosis is in part mediated via these receptors (Figure 3).
The possibility of a metabotropic taurine receptor that is coupled to an inhibitory G-protein (Foos and Wu 2002 ) resulting in reduction of ER calcium release, maintaining the ER’s calcium homeostasis in the ischemic brain (Figure 3), could provide insights into taurine’s mechanism in reducing ER stress and ER stress-mediated apoptosis [73,190]. The neuroprotective mechanisms of taurine address the diverse pathological mechanisms observed in ischemic stroke. Taurine not only addresses stroke pathology but it also extends the therapeutic window in which a compound maybe effective. Sun and colleagues, using an experimental rat model of stroke demonstrated that intravenous administration of taurine (50 mg/kg) was neuroprotective up to 8 h after ischemia . They attributed the suppression of neutrophil infiltration as one of the neuroprotective mechanisms of delayed taurine administration. Several preclinical studies have provided substantiating evidence of taurine’s neuroprotective mechanism against cerebral ischemia but for taurine to successfully translate to clinical trials of stroke patients, more preclinical research needs to be carried out that rigorously meets the STAIR criteria for a neuroprotective agent in cerebral stroke research . On the other hand, taurine has had success in clinical trials on congestive heart failure (CHF) [229,230,231,232,233] where the specific dosage and length of administration did not produce any adverse side effects. This should alert clinical stroke researchers to the feasibility of applying the CHF clinical paradigm to cerebral stroke patients.
This work was supported, in part, by the James and Esther King Biomedical Research Program, Florida Department of Health (grant # 09KW-11), and the Schmidt Foundation, Charles E., Schmidt College of Medicine, Florida Atlantic University.
Conflict of Interest
The authors have no conflict of interest to declare.
- Feigin, V.L.; Lawes, C.M.M.; Bennett, D.A.; Barker-Collo, S.L.; Parag, V. Worldwide stroke incidence and early case fatality reported in 56 population-based studies: A systematic review. Lancet Neurol. 2009, 8, 355–369, doi:10.1016/S1474-4422(09)70025-0.
- Strong, K.; Mathers, C.; Bonita, R. Preventing stroke: Saving lives around the world. Lancet Neurol. 2007, 6, 182–187, doi:10.1016/S1474-4422(07)70031-5.
- MacKay, J.; Mensah, G. The Atlast of Heart Disease and Stroke; World Health Organization: Geneva, Switzerland, 2004; pp. 50–53.
- Tackling the global burden of stroke. Lancet Neurol. 2005, 4, 689, doi:10.1016/S1474-4422(05)70202-7.
- Rosamond, W.; Flegal, K.; Friday, G.; Furie, K.; Go, A.; Greenlund, K.; Haase, N.; Ho, M.; Howard, V.; Kissela, B.; et al. Heart disease and stroke statistics—2007 update: A report from the American Heart Association Statistics Committee and Stroke Statistics Subcommittee. Circulation 2007, 115, 169–171.
- Kirino, Y.; Yasukawa, T.; Ohta, S.; Akira, S.; Ishihara, K.; Watanabe, K.; Suzuki, T. Codon-specific translational defect caused by a wobble modification deficiency in mutant tRNA from a human mitochondrial disease. Proc. Natl. Acad. Sci. USA 2004, 101, 15070–15075, doi:10.1073/pnas.0405173101.
- Busl, K.M.; Greer, D.M. Hypoxic-ischemic brain injury: Pathophysiology, neuropathology and mechanims. NeuroRehabilitation 2010, 26, 5–13.
- Hossmann, K.A. Pathophysiology and therapy of experimental stroke. Cell. Mol. Neurobiol. 2006, 26, 1057–1083, doi:10.1007/s10571-006-9008-1.
- Hossmann, K.A. Viability thresholds and the penumbra of focal ischemia. Ann. Neurol. 1994, 36, 557–565, doi:10.1002/ana.410360404.
- Heiss, W.D.; Grond, M.; Thiel, A.; Von Stockhausen, H.M.; Rudolf, J.; Ghaemi, M.; Löttgen, J.; Stenzel, C.; Pawlik, G. Tissue at risk of infarction rescued by early reperfusion: A positron emission tomography study in systemic recombinant tissue plasminogen activator thrombolysis of acute stroke. J. Cereb. Blood Flow Metab. 1998, 18, 1298–1307.
- Obrenovitch, T.P. The ischaemic penumbra: Twenty years on. Cerebrovasc. Brain Metab. Rev. 1995, 7, 297–323.
- Fisher, M.; Garcia, J.H. Evolving stroke and the ischemic penumbra. Neurology 1996, 47, 884–888, doi:10.1212/WNL.47.4.884.
- The National Institute of Neurological Disorders and Stroke (NINDS) rt-PA Stroke Study Group. Tissue Plasminogen Activator for Acute Ischemic Stroke. N. Engl. J. Med. 1995, 333, 1581–1588, doi:10.1056/NEJM199512143332401.
- Hacke, W.; Kaste, M.; Bluhmki, E.; Brozman, M.; Dávalos, A.; Guidetti, D.; Larrue, V.; Lees, K.R.; Medeghri, Z.; Machnig, T.; et al. Thrombolysis with alteplase 3 to 4.5 hours after acute ischemic stroke. N. Engl. J. Med. 2008, 359, 1317–1329, doi:10.1056/NEJMoa0804656.
- Jacobsen, J.G.; Smith, L.H. Biochemistry and physiology of taurine and taurine derivatives. Physiol. Rev. 1968, 48, 424–511.
- Reichelt, K.L.; Edminson, P.D. Biogenic amine specificity of cortical peptide synthesis in monkey brain. FEBS Lett. 1974, 47, 185–189, doi:10.1016/0014-5793(74)80455-2.
- Macaione, S.; Ruggeri, P.; De Luca, F.; Tucci, G. Free Amino Acids in Developing Rat Retina. J. Neurochem. 1974, 22, 887–891, doi:10.1111/j.1471-4159.1974.tb04313.x.
- Huxtable, R.J. Metabolism and function of taurine in the heart. In Taurine; Raven Press: New York, NY, USA, 1976; pp. 99–119.
- Huxtable, R.J. Physiological actions of taurine. Physiol. Rev. 1992, 72, 101–163.
- Saransaari, P.; Oja, S.S. Taurine and neural cell damage. Amino Acids 2000, 19, 509–526, doi:10.1007/s007260070003.
- Warskulat, U.; Flögel, U.; Jacoby, C.; Hartwig, H.-G.; Thewissen, M.; Merx, M.W.; Molojavyi, A.; Heller-Stilb, B.; Schrader, J.; Häussinger, D. Taurine transporter knockout depletes muscle taurine levels and results in severe skeletal muscle impairment but leaves cardiac function uncompromised. FASEB J. 2004, 18, 577–579.
- Oja, S.S.; Saransaari, P. Pharmacology of Taurine. Proc. West. Pharmacol. Soc. 2007, 50, 8–15.
- Wu, J.Y. Purification and characterization of cysteic acid and cysteine sulfinic acid decarboxylase and l-glutamate decarboxylase from bovine brain. Proc. Natl. Acad. Sci. USA 1982, 79, 4270–4274, doi:10.1073/pnas.79.14.4270.
- Hayes, K.C. Taurine requirement in primates. Nutr. Rev. 1985, 43, 65–70, doi:10.1111/j.1753-4887.1985.tb06862.x.
- Stapleton, P.P.; Charles, R.P.; Redmond, H.P.; Bouchier-Hayes, D.J. Taurine and human nutrition. Clin. Nutr. 1997, 16, 103–108, doi:10.1016/S0261-5614(97)80234-8.
- Yamori, Y.; Taguchi, T.; Hamada, A.; Kunimasa, K.; Mori, H.; Mori, M. Taurine in health and diseases: Consistent evidence from experimental and epidemiological studies. J. Biomed. Sci. 2010, 17, S6, doi:10.1186/1423-0127-17-S1-S6.
- Curtis, D.R.; Watkins, J.C. The pharmacology of amino acids related to gamma-aminobutyric acid. Pharmacol. Rev. 1965, 17, 347–391.
- Davison, A.N.; Kaczmarek, L.K. Taurine—a possible neurotransmitter? Nature 1971, 234, 107–108, doi:10.1038/234107a0.
- Hayes, K.C.; Carey, R.E.; Schmidt, S.Y. Retinal degeneration associated with taurine deficiency in the cat. Science 1975, 188, 949–951.
- Imaki, H.; Jacobson, S.G.; Kemp, C.M.; Knighton, R.W.; Neuringer, M.; Sturman, J. Retinal morphology and visual pigment levels in 6- and 12-month-old rhesus monkeys fed a taurine-free human infant formula. J. Neurosci. Res. 1993, 36, 290–304, doi:10.1002/jnr.490360307.
- Militante, J.D.; Lombardini, J.B. Taurine: Evidence of physiological function in the retina. Nutr. Neurosci. 2002, 5, 75–90, doi:10.1080/10284150290018991.
- Bulley, S.; Liu, Y.; Ripps, H.; Shen, W. Taurine activates delayed rectifier KV channels via a metabotropic pathway in retinal neurons. J. Physiol. 2013, 591, 123–132, doi:10.1113/jphysiol.2012.243147.
- Ito, T.; Kimura, Y.; Uozumi, Y.; Takai, M.; Muraoka, S.; Matsuda, T.; Ueki, K.; Yoshiyama, M.; Ikawa, M.; Okabe, M.; Schaffer, S.W.; Fujio, Y.; Azuma, J. Taurine depletion caused by knocking out the taurine transporter gene leads to cardiomyopathy with cardiac atrophy. J. Mol. Cell. Cardiol. 2008, 44, 927–937, doi:10.1016/j.yjmcc.2008.03.001.
- Schaffer, S.W.; Jong, C.J.; Ramila, K.C.; Azuma, J. Physiological roles of taurine in heart and muscle. J. Biomed. Sci. 2010, 17, S2, doi:10.1186/1423-0127-17-S1-S2.
- Sturman, J.A. Taurine in Development. Physiol. Rev. 1993, 73, 119–147.
- L’Amoreaux, W.J.; Cuttitta, C.; Santora, A.; Blaize, J.F.; Tachjadi, J.; El Idrissi, A. Taurine regulates insulin release from pancreatic beta cell lines. J. Biomed. Sci. 2010, 17, S11, doi:10.1186/1423-0127-17-S1-S11.
- Sturman, J.A.; Moretz, R.C.; French, J.H.; Wisniewski, H.M. Taurine deficiency in the developing cat: Persistence of the cerebellar external granule cell layer. J. Neurosci. Res. 1985, 13, 405–416, doi:10.1002/jnr.490130307.
- Sturman, J.A.; Moretz, R.C.; French, J.H.; Wisniewski, H.M. Postnatal taurine deficiency in the kitten results in a persistence of the cerebellar external granule cell layer: Correction by taurine feeding. J. Neurosci. Res. 1985, 13, 521–528, doi:10.1002/jnr.490130407.
- Neuringer, M.; Palackal, T.; Kujawa, M.; Moretz, R.C.; Sturman, J.A. Visual cortex development in rhesus monkeys deprived of dietary taurine. Prog. Clin. Biol. Res. 1990, 351, 415–422.
- Hernández-Benítez, R.; Pasantes-Morales, H.; Saldaña, I.T.; Ramos-Mandujano, G. Taurine stimulates proliferation of mice embryonic cultured neural progenitor cells. J. Neurosci. Res. 2010, 88, 1673–1681.
- Hernández-Benítez, R.; Ramos-Mandujano, G.; Pasantes-Morales, H. Taurine stimulates proliferation and promotes neurogenesis of mouse adult cultured neural stem/progenitor cells. Stem Cell Res. 2012, 9, 24–34, doi:10.1016/j.scr.2012.02.004.
- Bianchi, L.; Colivicchi, M.A.; Ballini, C.; Fattori, M.; Venturi, C.; Giovannini, M.G.; Healy, J.; Tipton, K.F.; Della Corte, L. Taurine, taurine analogues, and taurine functions: Overview. Adv. Exp. Med. Biol. 2006, 583, 443–448, doi:10.1007/978-0-387-33504-9_51.
- Ripps, H.; Shen, W. Review: Taurine: A “very essential” amino acid. Mol. Vis. 2012, 18, 2673–2686.
- Anuradha, C.V.; Balakrishnan, S.D. Taurine attenuates hypertension and improves insulin sensitivity in the fructose-fed rat, an animal model of insulin resistance. Can. J. Physiol. Pharmacol. 1999, 77, 749–754, doi:10.1139/y99-060.
- Nakaya, Y.; Minami, A.; Harada, N.; Sakamoto, S.; Niwa, Y.; Ohnaka, M. Taurine improves insulin sensitivity in the Otsuka Long-Evans Tokushima Fatty rat, a model of spontaneous type 2 diabetes. Am. J. Clin. Nutr. 2000, 71, 54–58.
- Ito, T.; Schaffer, S.W.; Azuma, J. The potential usefulness of taurine on diabetes mellitus and its complications. Amino Acids 2012, 42, 1529–1539, doi:10.1007/s00726-011-0883-5.
- Murakami, S. Taurine and atherosclerosis. Amino Acids 2012, doi:10.1007/s00726-012-1432-6.
- Menzie, J.; Pan, C.; Prentice, H.; Wu, J.-Y. Taurine and central nervous system disorders. Amino Acids 2012, doi:10.1007/s00726-012-1382-z.
- Pion, P.D.; Kittleson, M.D.; Rogers, Q.R.; Morris, J.G. Myocardial failure in cats associated with low plasma taurine: A reversible cardiomyopathy. Science 1987, 237, 764–768.
- Zulli, A. Taurine in cardiovascular disease. Curr. Opin. Clin. Nutr. Metab. Care 2011, 14, 57–60, doi:10.1097/MCO.0b013e328340d863.
- Tsuji, A.; Tamai, I. Sodium- and chloride-dependent transport of taurine at the blood-brain barrier. Adv. Exp. Med. Biol. 1996, 403, 385–391.
- Salimäki, J.; Scriba, G.; Piepponen, T.P.; Rautolahti, N.; Ahtee, L. The effects of systemically administered taurine and N-pivaloyltaurine on striatal extracellular dopamine and taurine in freely moving rats. Naunyn Schmiedebergs Arch. Pharmacol. 2003, 368, 134–141, doi:10.1007/s00210-003-0776-6.
- Huxtable, R.J. Taurine in the central nervous system and the mammalian actions of taurine. Prog. Neurobiol. 1989, 32, 471–533, doi:10.1016/0301-0082(89)90019-1.
- Wu, J.-Y.; Prentice, H. Role of taurine in the central nervous system. J. Biomed. Sci. 2010, 17, S1, doi:10.1186/1423-0127-17-S1-S1.
- Kuriyama, K. Taurine as a neuromodulator. Fed. Proc. 1980, 39, 2680–2684.
- El Idrissi, A.; Trenkner, E. Taurine as a modulator of excitatory and inhibitory neurotransmission. Neurochem. Res. 2004, 29, 189–197, doi:10.1023/B:NERE.0000010448.17740.6e.
- Banerjee, R.; Vitvitsky, V.; Garg, S.K. The undertow of sulfur metabolism on glutamatergic neurotransmission. Trends Biochem. Sci. 2008, 33, 413–419, doi:10.1016/j.tibs.2008.06.006.
- Saransaari, P.; Oja, S.S. Taurine release in mouse brain stem slices under cell-damaging conditions. Amino Acids 2007, 32, 439–446, doi:10.1007/s00726-006-0375-1.
- Chen, W.Q.; Jin, H.; Nguyen, M.; Carr, J.; Lee, Y.J.; Hsu, C.C.; Faiman, M.D.; Schloss, J.V.; Wu, J.Y. Role of taurine in regulation of intracellular calcium level and neuroprotective function in cultured neurons. J. Neurosci. Res. 2001, 66, 612–619, doi:10.1002/jnr.10027.
- Foos, T.M.; Wu, J.-Y. The role of taurine in the central nervous system and the modulation of intracellular calcium homeostasis. Neurochem. Res. 2002, 27, 21–26, doi:10.1023/A:1014890219513.
- El Idrissi, A.; Trenkner, E. Growth factors and taurine protect against excitotoxicity by stabilizing calcium homeostasis and energy metabolism. J. Neurosci. 1999, 19, 9459–9468.
- El Idrissi, A.; Trenkner, E. Taurine regulates mitochondrial calcium homeostasis. Adv. Exp. Med. Biol. 2003, 526, 527–536.
- El Idrissi, A. Taurine increases mitochondrial buffering of calcium: Role in neuroprotection. Amino Acids 2008, 34, 321–328, doi:10.1007/s00726-006-0396-9.
- Moran, J.; Salazar, P.; Pasantes-Morales, H. Effect of tocopherol and taurine on membrane fluidity of retinal rod outer segments. Exp. Eye Res. 1987, 45, 769–776, doi:10.1016/S0014-4835(87)80094-5.
- Hagar, H.H. The protective effect of taurine against cyclosporine A-induced oxidative stress and hepatotoxicity in rats. Toxicol. Lett. 2004, 151, 335–343, doi:10.1016/j.toxlet.2004.03.002.
- Schaffer, S.W.; Azuma, J.; Mozaffari, M. Role of antioxidant activity of taurine in diabetes. Can. J. Physiol. Pharmacol. 2009, 87, 91–99, doi:10.1139/Y08-110.
- Miao, J.; Zhang, J.; Zheng, L.; Yu, X.; Zhu, W.; Zou, S. Taurine attenuates Streptococcus uberis-induced mastitis in rats by increasing T regulatory cells. Amino Acids 2012, 42, 2417–2428, doi:10.1007/s00726-011-1047-3.
- Sun, M.; Zhao, Y.; Gu, Y.; Xu, C. Anti-inflammatory mechanism of taurine against ischemic stroke is related to down-regulation of PARP and NF-κB. Amino Acids 2012, 42, 1735–1747, doi:10.1007/s00726-011-0885-3.
- Wu, H.; Jin, Y.; Wei, J.; Jin, H.; Sha, D.; Wu, J.-Y. Mode of action of taurine as a neuroprotector. Brain Res. 2005, 1038, 123–131, doi:10.1016/j.brainres.2005.01.058.
- Leon, R.; Wu, H.; Jin, Y.; Wei, J.; Buddhala, C.; Prentice, H.; Wu, J.-Y. Protective function of taurine in glutamate-induced apoptosis in cultured neurons. J. Neurosci. Res. 2009, 87, 1185–1194, doi:10.1002/jnr.21926.
- Junyent, F.; Romero, R.; de Lemos, L.; Utrera, J.; Camins, A.; Pallàs, M.; Auladell, C. Taurine treatment inhibits CaMKII activity and modulates the presence of calbindin D28k, calretinin, and parvalbumin in the brain. J. Neurosci. Res. 2010, 88, 136–142, doi:10.1002/jnr.22192.
- Pan, C.; Giraldo, G.S.; Prentice, H.; Wu, J.-Y. Taurine protection of PC12 cells against endoplasmic reticulum stress induced by oxidative stress. J. Biomed. Sci. 2010, 17, S17, doi:10.1186/1423-0127-17-S1-S17.
- Pan, C.; Prentice, H.; Price, A.L.; Wu, J.-Y. Beneficial effect of taurine on hypoxia- and glutamate-induced endoplasmic reticulum stress pathways in primary neuronal culture. Amino Acids 2012, 43, 845–855.
- Lima, L.; Cubillos, S. Taurine might be acting as a trophic factor in the retina by modulating phosphorylation of cellular proteins. J. Neurosci. Res. 1998, 53, 377–384, doi:10.1002/(SICI)1097-4547(19980801)53:3<377::AID-JNR12>3.0.CO;2-2.
- Kontro, P.; Oja, S.S. Sodium-independent taurine binding to brain synaptic membranes. Cell. Mol. Neurobiol. 1983, 3, 183–187.
- Wu, J.Y.; Moss, L.G.; Chen, M.S. Tissue and regional distribution of cysteic acid decarboxylase. A new assay method. Neurochem. Res. 1979, 4, 201–212, doi:10.1007/BF00964144.
- Magnusson, K.R.; Clements, J.R.; Wu, J.Y.; Beitz, A.J. Colocalization of taurine- and cysteine sulfinic acid decarboxylase-like immunoreactivity in the hippocampus of the rat. Synapse 1989, 4, 55–69, doi:10.1002/syn.890040107.
- Philibert, R.A.; Rogers, K.L.; Dutton, G.R. Stimulus-coupled taurine efflux from cerebellar neuronal cultures: On the roles of Ca2+ and Na+. J. Neurosci. Res. 1989, 22, 167–171, doi:10.1002/jnr.490220209.
- Martin, D.L. Synthesis and release of neuroactive substances by glial cells. Glia 1992, 5, 81–94, doi:10.1002/glia.440050202.
- Kozlowski, D.J.; Chen, Z.; Zhuang, L.; Fei, Y.J.; Navarre, S.; Ganapathy, V. Molecular characterization and expression pattern of taurine transporter in zebrafish during embryogenesis. Life Sci. 2008, 82, 1004–1011, doi:10.1016/j.lfs.2008.02.015.
- Kang, Y.-S. Downregulation of taurine transport by calcium blockers in osteoblast cells. Adv. Exp. Med. Biol. 2009, 643, 513–521, doi:10.1007/978-0-387-75681-3_53.
- Okamoto, K.; Kimura, H.; Sakai, Y. Taurine-induced increase of the Cl-conductance of cerebellar Purkinje cell dendrites in vitro. Brain Res. 1983, 259, 319–323, doi:10.1016/0006-8993(83)91266-0.
- Del Olmo, N.; Bustamante, J.; Del Río, R.M.; Solís, J.M. Taurine activates GABA(A) but not GABA(B) receptors in rat hippocampal CA1 area. Brain Res. 2000, 864, 298–307, doi:10.1016/S0006-8993(00)02211-3.
- Albrecht, J.; Schousboe, A. Taurine interaction with neurotransmitter receptors in the CNS: An update. Neurochem. Res. 2005, 30, 1615–1621, doi:10.1007/s11064-005-8986-6.
- Wu, J.; Kohno, T.; Georgiev, S.K.; Ikoma, M.; Ishii, H.; Petrenko, A.B.; Baba, H. Taurine activates glycine and gamma-aminobutyric acid A receptors in rat substantia gelatinosa neurons. Neuroreport 2008, 19, 333–337, doi:10.1097/WNR.0b013e3282f50c90.
- Wu, J.Y.; Tang, X.W.; Tsai, W.H. Taurine receptor: Kinetic analysis and pharmacological studies. Adv. Exp. Med. Biol. 1992, 315, 263–268.
- Frosini, M.; Sesti, C.; Saponara, S.; Ricci, L.; Valoti, M.; Palmi, M.; Machetti, F.; Sgaragli, G. A specific taurine recognition site in the rabbit brain is responsible for taurine effects on thermoregulation. Br. J. Pharmacol. 2003, 139, 487–494, doi:10.1038/sj.bjp.0705274.
- Astrup, J.; Siesjö, B.K.; Symon, L. Thresholds in cerebral ischemia—the ischemic penumbra. Stroke 1981, 12, 723–725, doi:10.1161/01.STR.12.6.723.
- Kristian, T.; Siesjo, B.K. Calcium in ischemic cell death. Stroke 1998, 29, 705–718.
- Martin, R.L.; Lloyd, H.G.; Cowan, A.I. The early events of oxygen and glucose deprivation: Setting the scene for neuronal death? Trends Neurosci. 1994, 17, 251–257, doi:10.1016/0166-2236(94)90008-6.
- Katsura, K.; Kristian, T.; Siesjo, B.K. Energy metabolism, ion homeostasis, and cell damage in the brain. Biochem. Soc. Trans. 1994, 22, 991–996.
- Seki, Y.; Feustel, P.J.; Keller, R.W.; Tranmer, B.I.; Kimelberg, H.K.; Robinson, S.E. Inhibition of Ischemia-Induced Glutamate Release in Rat Striatum by Dihydrokinate and an Anion Channel Blocker Editorial Comment. Stroke 1999, 30, 433–440, doi:10.1161/01.STR.30.2.433.
- Globus, M.Y.-T.; Busto, R.; Dietrich, W.D.; Martinez, E.; Valdes, I.; Ginsberg, M.D. Effect of Ischemia on the in Vivo Release of Striatal Dopamine, Glutamate, and γ-Aminobutyric Acid Studied by Intracerebral Microdialysis. J. Neurochem. 1988, 51, 1455–1464, doi:10.1111/j.1471-4159.1988.tb01111.x.
- Hillered, L.; Hallström, A.; Segersvärd, S.; Persson, L.; Ungerstedt, U. Dynamics of extracellular metabolites in the striatum after middle cerebral artery occlusion in the rat monitored by intracerebral microdialysis. J. Cereb. Blood Flow Metab. 1989, 9, 607–616, doi:10.1038/jcbfm.1989.87.
- Butcher, S.P.; Bullock, R.; Graham, D.I.; McCulloch, J. Correlation between amino acid release and neuropathologic outcome in rat brain following middle cerebral artery occlusion. Stroke 1990, 21, 1727–1733, doi:10.1161/01.STR.21.12.1727.
- Yang, G.Y.; Betz, A.L. Reperfusion-induced injury to the blood-brain barrier after middle cerebral artery occlusion in rats. Stroke 1994, 25, 1658–1664, doi:10.1161/01.STR.25.8.1658.
- O’Regan, M.H.; Smith-Barbour, M.; Perkins, L.M.; Phillis, J.W. A possible role for phospholipases in the release of neurotransmitter amino acids from ischemic rat cerebral cortex. Neurosci. Lett. 1995, 185, 191–194, doi:10.1016/0304-3940(95)11259-Y.
- O’Regan, M.H.; Perkins, L.M.; Phillis, J.W. Arachidonic acid and lysophosphatidylcholine modulate excitatory transmitter amino acid release from the rat cerebral cortex. Neurosci. Lett. 1995, 193, 85–88, doi:10.1016/0304-3940(95)11672-J.
- Phillis, J.W.; Song, D.; O’Regan, M.H. Inhibition by anion channel blockers of ischemia-evoked release of excitotoxic and other amino acids from rat cerebral cortex. Brain Res. 1997, 758, 9–16, doi:10.1016/S0006-8993(97)00155-8.
- Rutledge, E.M.; Aschner, M.; Kimelberg, H.K. Pharmacological characterization of swelling-induced d-[3H]aspartate release from primary astrocyte cultures. Am. J. Physiol. Cell Physiol. 1998, 274, 1511–1520.
- Feustel, P.J.; Jin, Y.; Kimelberg, H.K. Volume-regulated anion channels are the predominant contributors to release of excitatory amino acids in the ischemic cortical penumbra. Stroke 2004, 35, 1164–1168, doi:10.1161/01.STR.0000124127.57946.a1.
- Szatkowski, M.; Barbour, B.; Attwell, D. Non-vesicular release of glutamate from glial cells by reversed electrogenic glutamate uptake. Nature 1990, 348, 443–446, doi:10.1038/348443a0.
- Rossi, D.J.; Oshima, T.; Attwell, D. Glutamate release in severe brain ischaemia is mainly by reversed uptake. Nature 2000, 403, 316–321, doi:10.1038/35002090.
- Reuter, H.; Philipson, K.D. Sodium-calcium exchanger overexpression in the heart—insights from a transgenic mouse model. Basic Res. Cardiol. 2002, 97, 131–135.
- Meldrum, B.S. Glutamate as a Neurotransmitter in the Brain: Review of Physiology and Pathology. J. Nutr. 2000, 130, 1007S–1015S.
- Kwak, S.; Weiss, J.H. Calcium-permeable AMPA channels in neurodegenerative disease and ischemia. Curr. Opin. Neurobiol. 2006, 16, 281–287, doi:10.1016/j.conb.2006.05.004.
- Berridge, M.J. Inositol Trisphosphate and Calcium Signaling. Ann. N. Y. Acad. Sci. 1995, 766, 31–43, doi:10.1111/j.1749-6632.1995.tb26646.x.
- Sukhareva, M.; Smith, S.V.; Maric, D.; Barker, J.L. Functional Properties of Ryanodine Receptors in Hippocampal Neurons Change during Early Differentiation in Culture. J. Neurophysiol. 2002, 88, 1077–1087.
- Paschen, W.; Mengesdorf, T. Endoplasmic reticulum stress response and neurodegeneration. Cell Calcium 2005, 38, 409–415, doi:10.1016/j.ceca.2005.06.019.
- Ding, D.; Moskowitz, S.I.; Li, R.; Lee, S.B.; Esteban, M.; Tomaselli, K.; Chan, J.; Bergold, P.J. Acidosis induces necrosis and apoptosis of cultured hippocampal neurons. Exp. Neurol. 2000, 162, 1–12.
- Yermolaieva, O.; Leonard, A.S.; Schnizler, M.K.; Abboud, F.M.; Welsh, M.J. Extracellular acidosis increases neuronal cell calcium by activating acid-sensing ion channel 1a. Proc. Natl. Acad. Sci. USA 2004, 101, 6752–6757.
- Araújo, I.M.; Verdasca, M.J.; Leal, E.C.; Bahr, B.A.; Ambrósio, A.F.; Carvalho, A.P.; Carvalho, C.M. Early calpain-mediated proteolysis following AMPA receptor activation compromises neuronal survival in cultured hippocampal neurons. J. Neurochem. 2004, 91, 1322–1331, doi:10.1111/j.1471-4159.2004.02811.x.
- Montague, J.; Gaido, M.; Frye, C.; Cidlowski, J. A calcium-dependent nuclease from apoptotic rat thymocytes is homologous with cyclophilin. Recombinant cyclophilins A, B, and C have nuclease activity. J. Biol. Chem. 1994, 269, 18877–18880.
- Farooqui, A.A.; Yang, H.-C.; Rosenberger, T.A.; Horrocks, L.A. Phospholipase A2 and Its Role in Brain Tissue. J. Neurochem. 2002, 69, 889–901, doi:10.1046/j.1471-4159.1997.69030889.x.
- Dykens, J.A. Isolated Cerebral and Cerebellar Mitochondria Produce Free Radicals when Exposed to Elevated Ca2+ and Na+: Implications for Neurodegeneration. J. Neurochem. 2002, 63, 584–591, doi:10.1046/j.1471-4159.1994.63020584.x.
- Lièvre, V.; Becuwe, P.; Bianchi, A.; Bossenmeyer-Pourié, C.; Koziel, V.; Franck, P.; Nicolas, M.; Dauça, M.; Vert, P.; Daval, J. Intracellular generation of free radicals and modifications of detoxifying enzymes in cultured neurons from the developing rat forebrain in response to transient hypoxia. Neuroscience 2001, 105, 287–297, doi:10.1016/S0306-4522(01)00189-0.
- Duan, Y.; Gross, R.A.; Sheu, S.-S. Ca2+-dependent generation of mitochondrial reactive oxygen species serves as a signal for poly(ADP-ribose) polymerase-1 activation during glutamate excitotoxicity. J. Physiol. 2007, 585, 741–758, doi:10.1113/jphysiol.2007.145409.
- Lipton, P. Ischemic Cell Death in Brain Neurons. Physiol. Rev. 1999, 79, 1431–1568.
- Nakka, V.P.; Gusain, A.; Mehta, S.L.; Raghubir, R. Molecular mechanisms of apoptosis in cerebral ischemia: Multiple neuroprotective opportunities. Mol. Neurobiol. 2008, 37, 7–38, doi:10.1007/s12035-007-8013-9.
- Pellegrini-Giampietro, D.E. The distinct role of mGlu1 receptors in post-ischemic neuronal death. Trends Pharmacol Sci. 2003, 24, 461–470, doi:10.1016/S0165-6147(03)00231-1.
- Yamori, Y.; Liu, L.; Mori, M.; Sagara, M.; Murakami, S.; Nara, Y.; Mizushima, S. Taurine as the nutritional factor for the longevity of the Japanese revealed by a world-wide epidemiological survey. Adv. Exp. Med. Biol. 2009, 643, 13–25, doi:10.1007/978-0-387-75681-3_2.
- Wu, J.Y.; Lin, C.T.; Johansen, F.F.; Liu, J.W. Taurine neurons in rat hippocampal formation are relatively inert to cerebral ischemia. Adv. Exp. Med. Biol. 1994, 359, 289–298.
- Schurr, A.; Rigor, B.M. The mechanism of neuronal resistance and adaptation to hypoxia. FEBS Lett. 1987, 224, 4–8, doi:10.1016/0014-5793(87)80411-8.
- Matsumoto, K.; Lo, E.H.; Pierce, A.R.; Halpern, E.F.; Newcomb, R. Secondary elevation of extracellular neurotransmitter amino acids in the reperfusion phase following focal cerebral ischemia. J. Cereb. Blood Flow Metab. 1996, 16, 114–124.
- Torp, R.; Andiné, P.; Hagberg, H.; Karagülle, T.; Blackstad, T.W.; Ottersen, O.P. Cellular and subcellular redistribution of glutamate-, glutamine- and taurine-like immunoreactivities during forebrain ischemia: A semiquantitative electron microscopic study in rat hippocampus. Neuroscience 1991, 41, 433–447, doi:10.1016/0306-4522(91)90339-P.
- Uchiyama-Tsuyuki, Y.; Araki, H.; Yae, T.; Otomo, S. Changes in the Extracellular Concentrations of Amino Acids in the Rat Striatum During Transient Focal Cerebral Ischemia. J. Neurochem. 2008, 62, 1074–1078, doi:10.1046/j.1471-4159.1994.62031074.x.
- Lo, E.; Pierce, A.; Matsumoto, K.; Kano, T.; Evans, C.; Newcomb, R. Alterations in K+ evoked profiles of neurotransmitter and neuromodulator amino acids after focal ischemia-reperfusion. Neuroscience 1997, 83, 449–458, doi:10.1016/S0306-4522(97)00434-X.
- Oja, S.S.; Saransaari, P. Modulation of taurine release by glutamate receptors and nitric oxide. Prog. Neurobiol. 2000, 62, 407–425, doi:10.1016/S0301-0082(00)00005-8.
- Barakat, L.; Wang, D.; Bordey, A. Carrier-mediated uptake and release of taurine from Bergmann glia in rat cerebellar slices. J. Physiol. 2002, 541, 753–767, doi:10.1113/jphysiol.2001.015834.
- Büyükuysal, R.L. Ischemia and reoxygenation induced amino acid release and tissue damage in the slices of rat corpus striatum. Amino Acids 2004, 27, 57–67.
- Saransaari, P.; Oja, S.S. Modulation of taurine release in ischemia by glutamate receptors in mouse brain stem slices. Amino Acids 2010, 38, 739–746, doi:10.1007/s00726-009-0278-z.
- Fariello, R.G.; Golden, G.T.; Pisa, M. Homotaurine (3 aminopropanesulfonic acid; 3APS) protects from the convulsant and cytotoxic effect of systemically administered kainic acid. Neurology 1982, 32, 241–245, doi:10.1212/WNL.32.3.241.
- French, E.D.; Vezzani, A.; Whetsell, W.O.; Schwarcz, R. Anti-excitotoxic actions of taurine in the rat hippocampus studied in vivo and in vitro. Adv. Exp. Med. Biol. 1986, 203, 349–362, doi:10.1007/978-1-4684-7971-3_26.
- Wu, J.Y.; Johansen, F.F.; Lin, C.T.; Liu, J.W. Taurine system in the normal and ischemic rat hippocampus. Adv. Exp. Med. Biol. 1987, 217, 265–274.
- Trenkner, E. The role of taurine and glutamate during early postnatal cerebellar development of normal and Weaver mutant mice. Adv. Exp. Med. Biol. 1990, 268, 239–244.
- Tang, X.W.; Deupree, D.L.; Sun, Y.; Wu, J.Y. Biphasic effect of taurine on excitatory amino acid-induced neurotoxicity. Adv. Exp. Med. Biol. 1996, 403, 499–505.
- Wu, J.; Chen, W.; Tang, X.W.; Jin, H.; Foos, T.; Schloss, J.V.; Davis, K.; Faiman, M.D.; Hsu, C. Mode of Action of Taurine and regulation Dynamics of Its Synthesis in the CNS. Adv. Exp. Med. Biol. 2000, 483, 35–44.
- Wu, J.-Y.; Wu, H.; Jin, Y.; Wei, J.; Sha, D.; Prentice, H.; Lee, H.-H.; Lin, C.-H.; Lee, Y.-H.; Yang, L.-L. Mechanism of neuroprotective function of taurine. Adv. Exp. Med. Biol. 2009, 643, 169–179, doi:10.1007/978-0-387-75681-3_17.
- Chen, W. Mode of Action of Taurine. Ph.D Thesis, University of Kansas, Lawrence, KS, USA, 2000.
- Schurr, A.; Tseng, M.T.; West, C.A.; Rigor, B.M. Taurine improves the recovery of neuronal function following cerebral hypoxia: An in vitro study. Life Sci. 1987, 40, 2059–2066, doi:10.1016/0024-3205(87)90098-1.
- Ricci, L.; Valoti, M.; Sgaragli, G.; Frosini, M. Protection by taurine of rat brain cortical slices against oxygen glucose deprivation- and reoxygenation-induced damage. Eur. J. Pharmacol. 2009, 621, 26–32, doi:10.1016/j.ejphar.2009.08.017.
- Zhao, P.; Huang, Y.L.; Cheng, J.S. Taurine antagonizes calcium overload induced by glutamate or chemical hypoxia in cultured rat hippocampal neurons. Neurosci. Lett. 1999, 268, 25–28, doi:10.1016/S0304-3940(99)00373-0.
- Wang, G.-H.; Jiang, Z.-L.; Fan, X.-J.; Zhang, L.; Li, X.; Ke, K.-F. Neuroprotective effect of taurine against focal cerebral ischemia in rats possibly mediated by activation of both GABAA and glycine receptors. Neuropharmacology 2007, 52, 1199–1209, doi:10.1016/j.neuropharm.2006.10.022.
- Molchanova, S.M.; Oja, S.S.; Saransaari, P. Taurine attenuates d-[3H]aspartate release evoked by depolarization in ischemic corticostriatal slices. Brain Res. 2006, 1099, 64–72, doi:10.1016/j.brainres.2006.04.105.
- Nicholls, D.G. Mitochondrial calcium function and dysfunction in the central nervous system. Biochim. Biophys. Acta 2009, 1787, 1416–1424, doi:10.1016/j.bbabio.2009.03.010.
- Brustovetsky, N.; Brustovetsky, T.; Jemmerson, R.; Dubinsky, J.M. Calcium-induced cytochrome c release from CNS mitochondria is associated with the permeability transition and rupture of the outer membrane. J. Neurochem. 2002, 80, 207–218, doi:10.1046/j.0022-3042.2001.00671.x.
- Bernardi, P.; Krauskopf, A.; Basso, E.; Petronilli, V.; Blachly-Dyson, E.; Blalchy-Dyson, E.; Di Lisa, F.; Forte, M.A. The mitochondrial permeability transition from in vitro artifact to disease target. FEBS J. 2006, 273, 2077–2099, doi:10.1111/j.1742-4658.2006.05213.x.
- Jemmerson, R.; Dubinsky, J.M.; Brustovetsky, N. Cytochrome C release from CNS mitochondria and potential for clinical intervention in apoptosis-mediated CNS diseases. Antioxid. Redox Signal. 2005, 7, 1158–1172, doi:10.1089/ars.2005.7.1158.
- Dedkova, E.N.; Ji, X.; Lipsius, S.L.; Blatter, L.A. Mitochondrial calcium uptake stimulates nitric oxide production in mitochondria of bovine vascular endothelial cells. Am J. Physiol. Cell Physiol. 2004, 286, 406–415, doi:10.1152/ajpcell.00155.2003.
- Ottersen, O.P. Quantitative assessment of taurine-like immunoreactivity in different cell types and processes in rat cerebellum: An electronmicroscopic study based on a postembedding immunogold labelling procedure. Anat. Embryol. 1988, 178, 407–421, doi:10.1007/BF00306047.
- Lobo, M.V.; Alonso, F.J.; Martin del Rio, R. Immunocytochemical localization of taurine in different muscle cell types of the dog and rat. Histochem. J. 2000, 32, 53–61, doi:10.1023/A:1003910429346.
- Bollard, M.E.; Murray, A.J.; Clarke, K.; Nicholson, J.K.; Griffin, J.L. A study of metabolic compartmentation in the rat heart and cardiac mitochondria using high-resolution magic angle spinning 1H NMR spectroscopy. FEBS Lett. 2003, 553, 73–78, doi:10.1016/S0014-5793(03)00969-4.
- Palmi, M.; Youmbi, G.T.; Fusi, F.; Sgaragli, G.P.; Dixon, H.B.; Frosini, M.; Tipton, K.F. Potentiation of mitochondrial Ca2+ sequestration by taurine. Biochem. Pharmacol. 1999, 58, 1123–1131, doi:10.1016/S0006-2952(99)00183-5.
- Hansen, S.H.; Andersen, M.L.; Birkedal, H.; Cornett, C.; Wibrand, F. The important role of taurine in oxidative metabolism. Adv. Exp. Med. Biol. 2006, 583, 129–135, doi:10.1007/978-0-387-33504-9_13.
- Chen, K.; Zhang, Q.; Wang, J.; Liu, F.; Mi, M.; Xu, H.; Chen, F.; Zeng, K. Taurine protects transformed rat retinal ganglion cells from hypoxia-induced apoptosis by preventing mitochondrial dysfunction. Brain Res. 2009, 1279, 131–138, doi:10.1016/j.brainres.2009.04.054.
- Hansen, S.H.; Andersen, M.L.; Cornett, C.; Gradinaru, R.; Grunnet, N. A role for taurine in mitochondrial function. J. Biomed. Sci. 2010, 17, S23, doi:10.1186/1423-0127-17-S1-S23.
- Suzuki, T.; Suzuki, T.; Wada, T.; Saigo, K.; Watanabe, K. Taurine as a constituent of mitochondrial tRNAs: New insights into the functions of taurine and human mitochondrial diseases. EMBO J. 2002, 21, 6581–6589, doi:10.1093/emboj/cdf656.
- Yasukawa, T.; Suzuki, T.; Ueda, T.; Ohta, S.; Watanabe, K. Modification defect at anticodon wobble nucleotide of mitochondrial tRNAs(Leu)(UUR) with pathogenic mutations of mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like episodes. J. Biol. Chem. 2000, 275, 4251–4257.
- Suzuki, T.; Nagao, A.; Suzuki, T. Human mitochondrial diseases caused by lack of taurine modification in mitochondrial tRNAs. Wiley Interdiscip. Rev. RNA 2011, 2, 376–386, doi:10.1002/wrna.65.
- Rikimaru, M.; Ohsawa, Y.; Wolf, A.M.; Nishimaki, K.; Ichimiya, H.; Kamimura, N.; Nishimatsu, S. Taurine Ameliorates Impaired the Mitochondrial Function and Prevents Stroke-like Episodes in Patients with MELAS. Intern. Med. 2012, 51, 3351–3357.
- Thornberry, N.A. Caspases: Enemies Within. Science 1998, 281, 1312–1316, doi:10.1126/science.281.5381.1312.
- Endres, M.; Namura, S.; Shimizu-Sasamata, M.; Waeber, C.; Zhang, L.; Gómez-Isla, T.; Hyman, B.T.; Moskowitz, M.A. Attenuation of delayed neuronal death after mild focal ischemia in mice by inhibition of the caspase family. J. Cereb. Blood Flow Metab. 1998, 18, 238–247.
- Rami, A.; Agarwal, R.; Botez, G.; Winckler, J. μ-Calpain activation, DNA fragmentation, and synergistic effects of caspase and calpain inhibitors in protecting hippocampal neurons from ischemic damage. Brain Res. 2000, 866, 299–312, doi:10.1016/S0006-8993(00)02301-5.
- Love, S. Apoptosis and brain ischaemia. Prog. Neuropsychopharmacol. Biol. Psychiatry 2003, 27, 267–282, doi:10.1016/S0278-5846(03)00022-8.
- Kambe, A.; Yokota, M.; Saido, T.C.; Satokata, I.; Fujikawa, H.; Tabuchi, S.; Kamitani, H.; Watanabe, T. Spatial resolution of calpain-catalyzed proteolysis in focal cerebral ischemia. Brain Res. 2005, 1040, 36–43, doi:10.1016/j.brainres.2005.01.080.
- Han, F.; Shirasaki, Y.; Fukunaga, K. 3-[2-[4-(3-Chloro-2-methylphenylmethyl)-1-piperazinyl]ethyl]-5,6-dimethoxy-1-(4-imidazolylmethyl)-1H-indazole dihydro-chloride 3.5 hydrate (DY-9760e) is neuroprotective in rat microsphere embolism: Role of the cross-talk between calpain and caspase-3 through calpastatin. J. Pharmacol. Exp. Ther. 2006, 317, 529–536, doi:10.1124/jpet.105.095018.
- Goll, D.E.; Thompson, V.F.; Li, H.; Wei, W.; Cong, J. The Calpain System. Physiol. Rev. 1990, 731–801.
- Wang, K.K. Calpain and caspase: Can you tell the difference? Trends Neurosci. 2000, 23, 20–26, doi:10.1016/S0166-2236(99)01479-4.
- Ling, Y.-H.; Liebes, L.; Ng, B.; Buckley, M.; Elliott, P.J.; Adams, J.; Jiang, J.-D.; Muggia, F.M.; Perez-Soler, R. PS-341, a Novel Proteasome Inhibitor, Induces Bcl-2 Phosphorylation and Cleavage in Association with G2-M Phase Arrest and Apoptosis. Mol. Cancer Ther. 2002, 1, 841–849.
- Nakagawa, T.; Yuan, J. Cross-Talk between Two Cysteine Protease Families: Activation of Caspase-12 by Calpain in Apoptosis. J. Cell Biol. 2000, 150, 887–894, doi:10.1083/jcb.150.4.887.
- Gao, G.; Dou, Q.P. N-terminal cleavage of bax by calpain generates a potent proapoptotic 18-kDa fragment that promotes bcl-2-independent cytochrome c release and apoptotic cell death. J. Cell Biochem. 2000, 80, 53–72, doi:10.1002/1097-4644(20010101)80:1<53::AID-JCB60>3.0.CO;2-E.
- Castro, R.E.; Solá, S.; Ramalho, R.M.; Steer, C.J.; Rodrigues, C.M.P. The bile acid tauroursodeoxycholic acid modulates phosphorylation and translocation of bad via phosphatidylinositol 3-kinase in glutamate-induced apoptosis of rat cortical neurons. J. Pharmacol. Exp. Ther. 2004, 311, 845–852, doi:10.1124/jpet.104.070532.
- Gil-Parrado, S.; Fernández-Montalván, A.; Assfalg-Machleidt, I.; Popp, O.; Bestvater, F.; Holloschi, A.; Knoch, T.A.; Auerswald, E.A.; Welsh, K.; Reed, J.C.; et al. Ionomycin-activated calpain triggers apoptosis. A probable role for Bcl-2 family members. J. Biol. Chem. 2002, 277, 27217–27226, doi:10.1074/jbc.M202945200.
- Salvesen, G.S.; Dixit, V.M. Caspases: Intracellular Signaling by Proteolysis. Cell 1997, 91, 443–446, doi:10.1016/S0092-8674(00)80430-4.
- Li, P.; Nijhawan, D.; Budihardjo, I.; Srinivasula, S.M.; Ahmad, M.; Alnemri, E.S.; Wang, X. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997, 91, 479–489, doi:10.1016/S0092-8674(00)80434-1.
- Takatani, T.; Takahashi, K.; Uozumi, Y.; Shikata, E.; Yamamoto, Y.; Ito, T.; Matsuda, T.; Schaffer, S.W.; Fujio, Y.; Azuma, J. Taurine inhibits apoptosis by preventing formation of the Apaf-1/caspase-9 apoptosome. Am. J. Physiol. Cell Physiol. 2004, 287, 949–953, doi:10.1152/ajpcell.00042.2004.
- Sun, M.; Xu, C. Neuroprotective mechanism of taurine due to up-regulating calpastatin and down-regulating calpain and caspase-3 during focal cerebral ischemia. Cell Mol. Neurobiol. 2008, 28, 593–611, doi:10.1007/s10571-007-9183-8.
- Sun, M.; Gu, Y.; Zhao, Y.; Xu, C. Protective functions of taurine against experimental stroke through depressing mitochondria-mediated cell death in rats. Amino Acids 2011, 40, 1419–1429, doi:10.1007/s00726-010-0751-8.
- Taranukhin, A.G.; Taranukhina, E.Y.; Saransaari, P.; Djatchkova, I.M.; Pelto-Huikko, M.; Oja, S.S. Taurine reduces caspase-8 and caspase-9 expression induced by ischemia in the mouse hypothalamic nuclei. Amino Acids 2008, 34, 169–174, doi:10.1007/s00726-006-0405-z.
- Kumar, R.; Azam, S.; Sullivan, J.M.; Owen, C.; Cavener, D.R.; Zhang, P.; Ron, D.; Harding, H.P.; Chen, J.-J.; Han, A.; et al. Brain ischemia and reperfusion activates the eukaryotic initiation factor 2α kinase, PERK. J. Neurochem. 2001, 77, 1418–1421, doi:10.1046/j.1471-4159.2001.00387.x.
- Azfer, A.; Niu, J.; Rogers, L.M.; Adamski, F.M.; Kolattukudy, P.E. Activation of endoplasmic reticulum stress response during the development of ischemic heart disease. Am. J. Physiol. Heart Circ. Physiol. 2006, 291, 1411–1420, doi:10.1152/ajpheart.01378.2005.
- Kuznetsov, G.; Brostrom, M.; Brostrom, C. Demonstration of a calcium requirement for secretory protein processing and export. Differential effects of calcium and dithiothreitol. J. Biol. Chem. 1992, 267, 3932–3939.
- Verkhratsky, A.; Toescu, E.C. Endoplasmic reticulum Ca(2+) homeostasis and neuronal death. J. Cell. Mol. Med. 2003, 7, 351–361, doi:10.1111/j.1582-4934.2003.tb00238.x.
- Pizzo, P.; Pozzan, T. Mitochondria-endoplasmic reticulum choreography: Structure and signaling dynamics. Trends Cell Biol. 2007, 17, 511–517, doi:10.1016/j.tcb.2007.07.011.
- Morimoto, N.; Oida, Y.; Shimazawa, M.; Miura, M.; Kudo, T.; Imaizumi, K.; Hara, H. Involvement of endoplasmic reticulum stress after middle cerebral artery occlusion in mice. Neuroscience 2007, 14, 957–967.
- Malhotra, J.D.; Kaufman, R.J. The endoplasmic reticulum and the unfolded proteinresponse. Semin. Cell Dev. Biol. 2007, 18, 716–731, doi:10.1016/j.semcdb.2007.09.003.
- Rao, R.V.; Bredesen, D.E. Misfolded proteins, endoplasmic reticulum stress and neurodegeneration. Curr. Opin. Cell Biol. 2004, 16, 653–662, doi:10.1016/j.ceb.2004.09.012.
- Ron, D.; Walter, P. Signal integration in the endoplasmic reticulum unfolded protein response. Nat. Rev. Mol. Cell Biol. 2007, 8, 519–529, doi:10.1038/nrm2199.
- Chakrabarti, A.; Chen, A.W.; Varner, J.D. A review of the mammalian unfolded protein response. Biotechnol. Bioeng. 2011, 108, 2777–2793, doi:10.1002/bit.23282.
- Gharibani, P.M.; Modi, J.; Pan, C.; Menzie, J.; Ma, Z.; Chen, P.C.; Tao, R.; Prentice, H.; Wu, J.Y. The Mechanism of Taurine Protection Against Endoplasmic recticulum Stress in an Animal Stroke Model of Cerebral Artery Occlusion and Stroke-Related Conditions in Primary Neuronal Cell Culture. Adv. Exp. Med. Biol. 2013, 776, 241–258, doi:10.1007/978-1-4614-6093-0_23.
- Ghosh, A.P.; Klocke, B.J.; Ballestas, M.E.; Roth, K.A. CHOP potentially co-operates with FOXO3a in neuronal cells to regulate PUMA and BIM expression in response to ER stress. PLoS One 2012, 7, e39586.
- McCullough, K.D.; Martindale, J.L.; Klotz, L.O.; Aw, T.Y.; Holbrook, N.J. Gadd153 sensitizes cells to endoplasmic reticulum stress by down-regulating Bcl2 and perturbing the cellular redox state. Mol. Cell. Biol. 2001, 21, 1249–1259, doi:10.1128/MCB.21.4.1249-1259.2001.
- Schuller-Levis, G.B.; Park, E. Taurine and Its Chloramine: Modulators of Immunity. Neurochem Res. 2004, 29, 117–126, doi:10.1023/B:NERE.0000010440.37629.17.
- Halliwell, B. Reactive oxygen species and the central nervous system. J. Neurochem. 1992, 59, 1609–1623, doi:10.1111/j.1471-4159.1992.tb10990.x.
- Zini, I.; Tomasi, A.; Grimaldi, R.; Vannini, V.; Agnati, L.F. Detection of free radicals during brain ischemia and reperfusion by spin trapping and microdialysis. Neurosci. Lett. 1992, 138, 279–282, doi:10.1016/0304-3940(92)90933-X.
- Sohal, R.S.; Orr, W.C. Relationship between antioxidants, prooxidants, and the aging proces. Ann. N. Y. Acad. Sci. 1992, 663, 74–84, doi:10.1111/j.1749-6632.1992.tb38651.x.
- Turrens, J.F. Mitochondrial formation of reactive oxygen species. J. Physiol. 2003, 552, 335–344, doi:10.1113/jphysiol.2003.049478.
- Brookes, P.S.; Darley-Usmar, V.M. Role of calcium and superoxide dismutase in sensitizing mitochondria to peroxynitrite-induced permeability transition. Am. J. Physiol. Heart Circ. Physiol. 2004, 286, 39–46, doi:10.1152/ajpheart.00742.2003.
- Wikström, M.; Saari, H. A spectral shift in cytochrome a induced by calcium ions. Biochim. Biophys. Acta 1975, 408, 170–179, doi:10.1016/0005-2728(75)90009-2.
- Jekabsone, A.; Ivanoviene, L.; Brown, G.C.; Borutaite, V. Nitric oxide and calcium together inactivate mitochondrial complex I and induce cytochrome c release. J. Mol. Cell. Cardiol. 2003, 35, 803–809, doi:10.1016/S0022-2828(03)00137-8.
- Grijalba, M.T.; Vercesi, A.E.; Schreier, S. Ca2+-induced increased lipid packing and domain formation in submitochondrial particles. A possible early step in the mechanism of Ca2+-stimulated generation of reactive oxygen species by the respiratory chain. Biochemistry 1999, 38, 13279–13287, doi:10.1021/bi9828674.
- Cleeter, M.W.; Cooper, J.M.; Darley-Usmar, V.M.; Moncada, S.; Schapira, A.H. Reversible inhibition of cytochrome c oxidase, the terminal enzyme of the mitochondrial respiratory chain, by nitric oxide. Implications for neurodegenerative diseases. FEBS Lett. 1994, 345, 50–54, doi:10.1016/0014-5793(94)00424-2.
- Zoccarato, F.; Cavallini, L.; Alexandre, A. Respiration-dependent removal of exogenous H2O2 in brain mitochondria: Inhibition by Ca2+. J. Biol. Chem. 2004, 279, 4166–4174, doi:10.1074/jbc.M308143200.
- Patterson, S.D.; Spahr, C.S.; Daugas, E.; Susin, S.A.; Irinopoulou, T.; Koehler, C.; Kroemer, G. Mass spectrometric identification of proteins released from mitochondria undergoing permeability transition. Cell Death Differ. 2000, 7, 137–144, doi:10.1038/sj.cdd.4400640.
- Ricci, C.; Pastukh, V.; Leonard, J.; Turrens, J.; Wilson, G.; Schaffer, D.; Schaffer, S.W. Mitochondrial DNA damage triggers mitochondrial-superoxide generation and apoptosis. Am. J. Physiol. 2008, 294, 413–422.
- Mochizuki, H.; Oda, H.; Yokogoshi, H. Dietary taurine alters ascorbic acid metabolism in rats fed diets containing polychlorinated biphenyls. J. Nutr. 2000, 130, 873–876.
- Vohra, B.P.; Hui, X. Taurine protects against carbon tetrachloride toxicity in the cultured neurons and in vivo. Arch. Physiol. Biochem. 2001, 109, 90–94, doi:10.1076/apab.18.104.22.16887.
- Balkan, J.; Kanbağli, O.; Aykaç-Toker, G.; Uysal, M. Taurine treatment reduces hepatic lipids and oxidative stress in chronically ethanol-treated rats. Biol. Pharm. Bull. 2002, 25, 1231–1233, doi:10.1248/bpb.25.1231.
- Mahalakshmi, K.; Pushpakiran, G.; Anuradha, C.V. Taurine prevents acrylonitrile-induced oxidative stress in rat brain. Pol. J. Pharmacol. 2003, 55, 1037–1043.
- Pushpakiran, G.; Mahalakshmi, K.; Anuradha, C.V. Taurine restores ethanol-induced depletion of antioxidants and attenuates oxidative stress in rat tissues. Amino Acids 2004, 27, 91–96.
- Aruoma, O.I.; Halliwell, B.; Hoey, B.M.; Butler, J. The antioxidant action of taurine, hypotaurine and their metabolic precursors. Biochem. J. 1988, 256, 251–255.
- Jong, C.J.; Azuma, J.; Schaffer, S. Mechanism underlying the antioxidant activity of taurine: Prevention of mitochondrial oxidant production. Amino Acids 2012, 42, 2223–2232, doi:10.1007/s00726-011-0962-7.
- Jong, C.J.; Ito, T.; Mozaffari, M.; Azuma, J.; Schaffer, S. Effect of beta-alanine treatment on mitochondrial taurine level and 5-taurinomethyluridine content. J. Biomed. Sci. 2010, 17, S25, doi:10.1186/1423-0127-17-S1-S25.
- Amantea, D.; Nappi, G.; Bernardi, G.; Bagetta, G.; Corasaniti, M.T. Post-ischemic brain damage: Pathophysiology and role of inflammatory mediators. FEBS J. 2009, 276, 13–26, doi:10.1111/j.1742-4658.2008.06766.x.
- Koh, S.-H.; Chang, D.-I.; Kim, H.-T.; Kim, J.; Kim, M.-H.; Kim, K. S.; Bae, I.; Kim, H.; Kim, D.W.; Kim, S.H. Effect of 3-aminobenzamide, PARP inhibitor, on matrix metalloproteinase-9 level in plasma and brain of ischemic stroke model. Toxicology 2005, 214, 131–139, doi:10.1016/j.tox.2005.06.023.
- Haddad, M.; Rhinn, H.; Bloquel, C.; Coqueran, B.; Szabó, C.; Plotkine, M.; Scherman, D.; Margaill, I. Anti-inflammatory effects of PJ34, a poly(ADP-ribose) polymerase inhibitor, in transient focal cerebral ischemia in mice. Br. J. Pharmacol. 2006, 149, 23–30, doi:10.1038/sj.bjp.0706837.
- Sun, M.; Zhao, Y.-M.; Gu, Y.; Xu, C. Therapeutic window of taurine against experimental stroke in rats. Transl. Res. 2012, 160, 223–229, doi:10.1016/j.trsl.2012.02.007.
- Walz, W.; Klimaszewski, A.; Paterson, I.A. Glial swelling in ischemia: A hypothesis. Dev. Neurosci. 1993, 15, 216–225, doi:10.1159/000111337.
- Haugstad, T.S.; Langmoen, I.A. Release of brain amino acids during hyposmolar stress and energy deprivation. J. Neurosurg. Anesthesiol. 1996, 8, 159–168, doi:10.1097/00008506-199604000-00011.
- Saransaari, P.; Oja, S.S. Taurine release is enhanced in cell-damaging conditions in cultured cerebral cortical astrocytes. Neurochem. Res. 1999, 24, 1523–1529, doi:10.1023/A:1021195830773.
- Estevez, A.Y.; O’Regan, M.H.; Song, D.; Phillis, J.W. Hyposmotically induced amino acid release from the rat cerebral cortex: Role of phospholipases and protein kinases. Brain Res. 1999, 844, 1–9, doi:10.1016/S0006-8993(99)01801-6.
- Inoue, H.; Mori, S.-I.; Morishima, S.; Okada, Y. Volume-sensitive chloride channels in mouse cortical neurons: Characterization and role in volume regulation. Eur. J. Neurosci. 2005, 21, 1648–1658, doi:10.1111/j.1460-9568.2005.04006.x.
- Molchanova, S.M.; Oja, S.S.; Saransaari, P. Properties of basal taurine release in the rat striatum in vivo. Adv. Exp. Med. Biol. 2006, 583, 365–375, doi:10.1007/978-0-387-33504-9_41.
- Inoue, H.; Okada, Y. Roles of volume-sensitive chloride channel in excitotoxic neuronal injury. J. Neurosci. 2007, 27, 1445–1455, doi:10.1523/JNEUROSCI.4694-06.2007.
- Shuaib, A. The role of taurine in cerebral ischemia: Studies in transient forebrain ischemia and embolic focal ischemia in rodents. Adv. Exp. Med. Biol. 2003, 526, 421–431, doi:10.1007/978-1-4615-0077-3_51.
- Kudo, Y.; Akiyoshi, E.; Akagi, H. Identification of two taurine receptor subtypes on the primary afferent terminal of frog spinal cord. Br. J. Pharmacol. 1988, 94, 1051–1056, doi:10.1111/j.1476-5381.1988.tb11621.x.
- Sanberg, P.R.; Willow, M. Dose-dependent effects of taurine on convulsions induced by hypoxia in the rat. Neurosci Lett. 1980, 16, 297–300, doi:10.1016/0304-3940(80)90014-2.
- Stummer, W.; Betz, A.L.; Shakui, P.; Keep, R.F. Blood-brain barrier taurine transport during osmotic stress and in focal cerebral ischemia. J. Cereb. Blood Flow. Metab. 1995, 15, 852–859, doi:10.1038/jcbfm.1995.106.
- Azuma, J.; Sawamura, A.; Awata, N.; Ohta, H.; Hamaguchi, T.; Haradam, H.; Takiharma, K.; Hasegawa, H.; Yamagami, T.; Ishiyama, T.; et al. Therapeutic Effect of Taurine in Congestive Heart Failure: A Double-Blind Crossover Trial. Clin. Cardiol. 1985, 8, 276–282, doi:10.1002/clc.4960080507.
- Azuma, J.; Sawamura, A.; Awata, N. Usefulness of taurine in chronic congestive heart failure and its prospective application. Jpn. Circ. J. 1992, 56, 95–99, doi:10.1253/jcj.56.95.
- Yamori, Y.; Nara, Y.; Mizushima, S.; Sawamura, M.; Horie, R. Nutritional factors for stroke and major cardiovascular diseases: International epidemiological comparison of dietary prevention. Health Rep. 1994, 6, 22–27.
- Jeejeebhoy, F.; Keith, M.; Freeman, M.; Barr, A.; McCall, M.; Kudan, R.; Mazer, D.; Errett, L. Nutritional supplementation with MyoVive repletes essential cardiac myocyte nutrients and reduces left ventricular dysfunction. Am. Heart J. 2002, 143, 10092–10100.
- Kingston, R.; Kelly, C.J.; Murray, P. The therapeutic role of taurine in ischaemia-reperfusion injury. Curr. Pharm. Des. 2004, 10, 2401–2410, doi:10.2174/1381612043384015.
- Fisher, M.; Feuerstein, G.; Howells, D.W.; Hurn, P.D.; Kent, T.A.; Savitz, S.I.; Lo, E.H.; STAIR Group. Update of the stroke therapy academic industry roundtable preclinical recommendations. Stroke 2009, 40, 2244–2250, doi:10.1161/STROKEAHA.108.541128.
- Kanthan, R.; Shuaib, A.; Griebel, R.; Miyashita, H. Intracerebral human microdialysis. In vivo study of an acute focal ischemic model of the human brain. Stroke 1995, 26, 870–873, doi:10.1161/01.STR.26.5.870.
- Zhang, M.; Bi, L.F.; Fang, J.H.; Su, X.L.; Da, G.L.; Kuwamori, T.; Kagamimori, S. Beneficial effects of taurine on serum lipids in overweight or obese non-diabetic subjects. Amino Acids 2004, 26, 267–271.
- Takahashi, H.; Mori, T.; Fujihira, E.; Nakazawa, M. Long-term feeding of taurine in rats. Pharmacometrics 1972, 6, 529–534.
- Takahashi, H.; Kaneda, S.; Fukuda, K.; Fujihira, E.; Nakazawa, M. Studies on the teratology and three generation reproduction of taurine in mice. Pharmacometrics 1972, 6, 535–540.
- Sturman, J.A.; Messing, J.M. High dietary taurine effects on feline tissue taurine concentrations and reproductive performance. J. Nutr. 1992, 122, 82–88.
- Furukawa, S.; Katto, M.; Kouyama, H.; Nishida, I.; Kikumori, M.; Taniguchi, Y.; Toda, T.; Araki, H. Repeated dose toxicity study of intravenous treatment with taurine for 13 weeks and recovery test for 5 weeks in rat. Jpn. Pharmacol. Ther. 1991, 19, 275–306.
- Cantafora, A.; Mantovani, A.; Masella, R.; Mechelli, L.; Alvaro, D. Effect of taurine administration on liver lipids in guinea pig. Experientia 1986, 42, 407–408, doi:10.1007/BF02118631.
- European Food Safety Authority (EFSA). EFSA adopts opinion on two ingredients commonly used in some energy drinks. 2009. Available online: http://www.efsa.europa.eu/en/press/news/ans090212.htm (accessed on 15 February 2013).
- Schaffer, S.; Takahashi, K.; Azuma, J. Role of osmoregulation in the actions of taurine. Amino Acids 2000, 19, 527–546, doi:10.1007/s007260070004.
- Morales, I.; Dopico, J.G.; Sabate, M.; Gonzalez-Hernandez, T.; Rodriguez, M. Substantia nigra osmoregulation: Taurine and ATP involvement. Am. J. Physiol. Cell Physiol. 2007, 292, 1934–1941.
- O’Byrne, M.B.; Tipton, K.F. Taurine-induced attenuation of MPP+ neurotoxicity in vitro: A possible role for the GABA(A) subclass of GABA receptors. J. Neurochem. 2000, 74, 2087–2093.
- Paula-Lima, A.C.; de Felice, F.G.; Brito-Moreira, J.; Ferreira, S.T. Activation of GABA(A) receptors by taurine and muscimol blocks the neurotoxicity of beta-amyloid in rat hippocampal and cortical neurons. Neuropharmacology 2005, 49, 1140–1148, doi:10.1016/j.neuropharm.2005.06.015.
- Chen, P.C.; Pan, C.; Gharibani, P.M.; Prentice, H.; Wu, J.Y. Taurine exerts robust protection against hypoxia and oxygen/glucose deprivation in human neuroblastoma cell culture. Adv. Exp. Med. Biol. 2013, 775, 167–175, doi:10.1007/978-1-4614-6130-2_14.
- Das, J.; Ghosh, J.; Manna, P.; Sil, P.C. Taurine suppresses doxorubicin-triggered oxidative stress and cardiac apoptosis in rat via up-regulation of PI3-K/Akt and inhibition of p53, p38-JNK. Biochem. Pharmacol. 2011, 81, 891–909, doi:10.1016/j.bcp.2011.01.008.
- Smith, K.E.; Borden, L.A.; Wang, C.H.; Hartig, P.R.; Branchek, T.A.; Weinshank, R.L. Cloning and expression of a high affinity taurine transporter from rat brain. Mol. Pharmacol. 1992, 42, 563–569.
- Pow, D.V.; Sullivan, R.; Reye, P.; Hermanussen, S. Localization of taurine transporters, taurine, and (3)H taurine accumulation in the rat retina, pituitary, and brain. Glia 2002, 37, 153–168, doi:10.1002/glia.10026.
- Wu, J.Y.; Liao, C.C.; Lin, C.J.; Lee, Y.H.; Ho, J.Y.; Tsai, W.H. Taurine receptor in the mammalian brain. Prog. Clin. Biol. Res. 1990, 351, 147–156.
- López-Colomé, A.M.; Fragoso, G.; Salceda, R. Taurine receptors in membranes from retinal pigment epithelium cells in culture. Neuroscience 1991, 41, 791–796, doi:10.1016/0306-4522(91)90369-Y.
- Sung, D.-Y.; Walthall, W.W.; Derby, C.D. Identification and partial characterization of putative taurine receptor proteins from the olfactory organ of the spiny lobster. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 1996, 115, 19–26, doi:10.1016/0305-0491(96)00083-1.
- Anderson, P.A.; Trapido-Rosenthal, H.G. Physiological and chemical analysis of neurotransmitter candidates at a fast excitatory synapse in the jellyfish Cyanea capillata (Cnidaria, Scyphozoa). Invert. Neurosci. 2009, 9, 167–173, doi:10.1007/s10158-009-0095-9.
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