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Appl. Sci. 2018, 8(5), 745; https://doi.org/10.3390/app8050745

Quantification of Two Fluorophores’ Concentration Ratio in a Mice Model in Preparation for a Proposed Method for Early Detection of Alzheimer’s Disease

1
Department of Biomedical Engineering, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel
2
Department of Clinical Microbiology and Immunology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel
3
Department of Electrical and Computer Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
This paper is an extended version with additional data and updated references of a paper published in the In Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XV conference, San Francisco, CA, USA, 7–8 February 2015.
*
Author to whom correspondence should be addressed.
Received: 27 March 2018 / Revised: 29 April 2018 / Accepted: 3 May 2018 / Published: 8 May 2018
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Abstract

Many biomedical applications require concentration measurements of biological compounds, which may be achieved using targeted fluorescent probes. It has been shown that the ratio between amyloid-Beta and tau protein in the cerebrospinal fluid (CSF) is a good indicator of incipient Alzheimer’s disease (AD). We have previously proposed a method that can accurately estimate the concentration ratio of these two proteins without the need to collect CSF samples: Fluorescent probes are injected to the blood and bind to the CSF biomarkers. A miniature needle with an optical fiber excites the fluorescent probes and collects the fluorescence emission. The concentration ratio between the proteins is estimated, and used for diagnosis of incipient AD. We present here the results of the method’s concentration ratio estimation during trials performed on mice. Miniature tubes containing two fluorescent probes in several concentration ratios were implanted in two locations in the mice: subcutaneously, and deeper in the abdomen. The fluorescent probes were excited and the fluorescence intensity was measured. The concentration ratios were estimated from the fluorescence intensities with an average error of less than 20%. This study is a step towards the demonstration of the method in in vivo studies in mice with markers in the bloodstream/CSF. View Full-Text
Keywords: fluorescence; disease markers; Alzheimer’s early diagnostics; CSF biomarkers fluorescence; disease markers; Alzheimer’s early diagnostics; CSF biomarkers
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Harbater, O.; Efrati, M.; Gannot, I. Quantification of Two Fluorophores’ Concentration Ratio in a Mice Model in Preparation for a Proposed Method for Early Detection of Alzheimer’s Disease. Appl. Sci. 2018, 8, 745.

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