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Appl. Sci. 2017, 7(2), 170; doi:10.3390/app7020170

Electrical Impedance Spectroscopy for Detection of Cells in Suspensions Using Microfluidic Device with Integrated Microneedles

1
Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, Skudai 81310, Johor, Malaysia
2
Department of Micro-Nano Systems Engineering, Nagoya University, Nagoya 464-8603, Japan
3
Faculty of Electrical Engineering, Universiti Teknologi Malaysia, Skudai 81310, Johor, Malaysia
*
Author to whom correspondence should be addressed.
Academic Editor: Fan-Gang Tseng
Received: 21 December 2016 / Revised: 26 January 2017 / Accepted: 5 February 2017 / Published: 10 February 2017
(This article belongs to the Section Nanotechnology and Applied Nanosciences)
View Full-Text   |   Download PDF [1446 KB, uploaded 14 February 2017]   |  

Abstract

In this study, we introduce novel method of flow cytometry for cell detection based on impedance measurements. The state of the art method for impedance flow cytometry detection utilizes an embedded electrode in the microfluidic to perform measurement of electrical impedance of the presence of cells at the sensing area. Nonetheless, this method requires an expensive and complicated electrode fabrication process. Furthermore, reuse of the fabricated electrode also requires an intensive and tedious cleaning process. Due to that, we present a microfluidic device with integrated microneedles. The two microneedles are placed at the half height of the microchannel for cell detection and electrical measurement. A commercially-available Tungsten needle was utilized for the microneedles. The microneedles are easily removed from the disposable PDMS (Polydimethylsiloxane) microchannel and can be reused with a simple cleaning process, such as washing by ultrasonic cleaning. Although this device was low cost, it preserves the core functionality of the sensor, which is capable of detecting passing cells at the sensing area. Therefore, this device is suitable for low-cost medical and food safety screening and testing process in developing countries. View Full-Text
Keywords: impedance flow cytometry; microneedle; cell concentration detection impedance flow cytometry; microneedle; cell concentration detection
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Mansor, M.A.; Takeuchi, M.; Nakajima, M.; Hasegawa, Y.; Ahmad, M.R. Electrical Impedance Spectroscopy for Detection of Cells in Suspensions Using Microfluidic Device with Integrated Microneedles. Appl. Sci. 2017, 7, 170.

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