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Appl. Sci. 2017, 7(10), 998; doi:10.3390/app7100998

Organic Electrochemical Transistor Microplate for Real-Time Cell Culture Monitoring

1
Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 61200 Brno, Czech Republic
2
Institute of Biophysics AS CR, Královopolská 135, 61265 Brno, Czech Republic
3
Department of Experimental Biology, Faculty of Science, Masaryk University, University Campus Bohunice, Kamenice 5, 62500 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
Received: 20 July 2017 / Revised: 26 August 2017 / Accepted: 18 September 2017 / Published: 27 September 2017
(This article belongs to the Section Materials)
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Abstract

Human cell cultures provide a potentially powerful means for pharmacological and toxicological research. A microplate with a multielectrode array of 96 organic electrochemical transistors (OECTs) based on the semiconductive polymer poly(3,4-ethylenedioxythio-phene):poly(styrene sulfonic acid) PEDOT:PSS was developed and fabricated by the screen printing method. It consists of a microplate of a 12 × 8 chimney–well array with transistors on the bottom. The OECT is circular with a channel of 1.5 mm2 in the centre surrounded by the circular gate electrode. The device is designed for electrogenic cell monitoring. Simulations with the electrolyte revealed good electrical characteristics and indicated the setup information of the experimental conditions. A transconductance of g = 1.4 mS was achieved in the wide range of gate voltages Vgs = ±0.4 V when the drain potential Vds = −0.735 V was set and the long term relaxation was compensated for. The time constant 0.15 s limited by the channel-electrolyte charge electrical double layer (EDL) capacitance was measured. The device was tested on a 3T3 fibroblast cell culture and the sudden environmental changes were recorded. The living cells can be observed on the channel of the OECT and during electrical stimulation by gate voltage, as well as during the source current response. View Full-Text
Keywords: OECT; screen printing; organic electrochemical transistor; PEDOT:PSS; microplate; multi-electrode array; cell culture OECT; screen printing; organic electrochemical transistor; PEDOT:PSS; microplate; multi-electrode array; cell culture
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MDPI and ACS Style

Salyk, O.; Víteček, J.; Omasta, L.; Šafaříková, E.; Stříteský, S.; Vala, M.; Weiter, M. Organic Electrochemical Transistor Microplate for Real-Time Cell Culture Monitoring. Appl. Sci. 2017, 7, 998.

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