Next Article in Journal
Biodegradable and Biocompatible Systems Based on Hydroxyapatite Nanoparticles
Next Article in Special Issue
Enhanced Radiation Therapy of Gold Nanoparticles in Liver Cancer
Previous Article in Journal
Flexural Capacity of a New Composite Beam with Concrete-Infilled Tubular Lower Flange
Previous Article in Special Issue
Tunable Degradation Rate and Favorable Bioactivity of Porous Calcium Sulfate Scaffolds by Introducing Nano-Hydroxyapatite
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Appl. Sci. 2017, 7(1), 59; doi:10.3390/app7010059

Aligned Nanofiber Topography Directs the Tenogenic Differentiation of Mesenchymal Stem Cells

1
Department of Large Animal Clinical Sciences, Marion duPont Scott Equine Medical Center, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Leesburg, VA 20176, USA
2
Department of Mechanical Engineering, College of Engineering, Virginia Tech, Blacksburg, VA 24061, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Daniel X.B. Chen
Received: 15 December 2016 / Revised: 21 December 2016 / Accepted: 23 December 2016 / Published: 6 January 2017
View Full-Text   |   Download PDF [6765 KB, uploaded 6 January 2017]   |  

Abstract

Tendon is commonly injured, heals slowly and poorly, and often suffers re-injury after healing. This is due to failure of tenocytes to effectively remodel tendon after injury to recapitulate normal architecture, resulting in poor mechanical properties. One strategy for improving the outcome is to use nanofiber scaffolds and mesenchymal stem cells (MSCs) to regenerate tendon. Various scaffold parameters are known to influence tenogenesis. We designed suspended and aligned nanofiber scaffolds with the hypothesis that this would promote tenogenesis when seeded with MSCs. Our aligned nanofibers were manufactured using the previously reported non-electrospinning Spinneret-based Tunable Engineered Parameters (STEP) technique. We compared parallel versus perpendicular nanofiber scaffolds with traditional flat monolayers and used cellular morphology, tendon marker gene expression, and collagen and glycosaminoglycan deposition as determinants for tendon differentiation. We report that compared with traditional control monolayers, MSCs grown on nanofibers were morphologically elongated with higher gene expression of tendon marker scleraxis and collagen type I, along with increased production of extracellular matrix components collagen (p = 0.0293) and glycosaminoglycan (p = 0.0038). Further study of MSCs in different topographical environments is needed to elucidate the complex molecular mechanisms involved in stem cell differentiation. View Full-Text
Keywords: tendon; differentiation; topography; scaffolds; nanofiber tendon; differentiation; topography; scaffolds; nanofiber
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Popielarczyk, T.L.; Nain, A.S.; Barrett, J.G. Aligned Nanofiber Topography Directs the Tenogenic Differentiation of Mesenchymal Stem Cells. Appl. Sci. 2017, 7, 59.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Appl. Sci. EISSN 2076-3417 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top