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Appl. Sci. 2016, 6(8), 229; doi:10.3390/app6080229

Chip-Based Cytometry Illuminated by a Blade-Shape Continuous Light for Multispectral Detection

1
Metal Industries Research & Development Centre, Kaohsiung 811, Taiwan
2
Department of Mechanical and Electro-Mechanical Engineering, National Sun Yat-sen University, Kaohsiung 804, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editors: Teen-Hang Meen and Totaro Imasaka
Received: 9 June 2016 / Revised: 4 August 2016 / Accepted: 9 August 2016 / Published: 13 August 2016
View Full-Text   |   Download PDF [4322 KB, uploaded 13 August 2016]   |  

Abstract

A high performance diascopic illumination configuration is presented for the simultaneous detection of cells and particles with different sizes and different fluorescence labels in a microchannel. In the proposed approach, the cells/particles are illuminated by an objective-type dark-field condenser equipped with a low-cost tungsten light source and are then characterized by extracting the side-scatter, absorbance, and fluorescence signals from the spectra obtained by a ultraviolet-visible-near infrared (UV-Vis-NIR) spectrometer. A modified computation model is adopted to improve the capability for discriminating more fluorescence dyes simultaneously. The feasibility of the proposed detection configuration is demonstrated by counting and classifying a mixed sample of green, red, and crimson fluorescent-labeled particles and non-labeled particles with various dimensions. The suitability of the proposed system for real-world cytometry applications is then evaluated by classifying a mixed bio-sample comprising of gastric epithelial (AGS) cells stained with Trypan-blue and Erythrosin-bluish dye, respectively. The results show that the cytometer enables the efficient detection, identification, and classification of mixed bio-samples without the need for spatial filters or delicate optical components. Consequently, the proposed system has significant potential for high-performance micro-flow cytometry applications. View Full-Text
Keywords: micro-flow cytometry; multispectral detection; dark-field illumination; diascopic configuration micro-flow cytometry; multispectral detection; dark-field illumination; diascopic configuration
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Lin, S.-W.; Lin, C.-H. Chip-Based Cytometry Illuminated by a Blade-Shape Continuous Light for Multispectral Detection. Appl. Sci. 2016, 6, 229.

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