Chip-Based Cytometry Illuminated by a Blade-Shape Continuous Light for Multispectral Detection
AbstractA high performance diascopic illumination configuration is presented for the simultaneous detection of cells and particles with different sizes and different fluorescence labels in a microchannel. In the proposed approach, the cells/particles are illuminated by an objective-type dark-field condenser equipped with a low-cost tungsten light source and are then characterized by extracting the side-scatter, absorbance, and fluorescence signals from the spectra obtained by a ultraviolet-visible-near infrared (UV-Vis-NIR) spectrometer. A modified computation model is adopted to improve the capability for discriminating more fluorescence dyes simultaneously. The feasibility of the proposed detection configuration is demonstrated by counting and classifying a mixed sample of green, red, and crimson fluorescent-labeled particles and non-labeled particles with various dimensions. The suitability of the proposed system for real-world cytometry applications is then evaluated by classifying a mixed bio-sample comprising of gastric epithelial (AGS) cells stained with Trypan-blue and Erythrosin-bluish dye, respectively. The results show that the cytometer enables the efficient detection, identification, and classification of mixed bio-samples without the need for spatial filters or delicate optical components. Consequently, the proposed system has significant potential for high-performance micro-flow cytometry applications. View Full-Text
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Lin, S.-W.; Lin, C.-H. Chip-Based Cytometry Illuminated by a Blade-Shape Continuous Light for Multispectral Detection. Appl. Sci. 2016, 6, 229.
Lin S-W, Lin C-H. Chip-Based Cytometry Illuminated by a Blade-Shape Continuous Light for Multispectral Detection. Applied Sciences. 2016; 6(8):229.Chicago/Turabian Style
Lin, Shi-Wei; Lin, Che-Hsin. 2016. "Chip-Based Cytometry Illuminated by a Blade-Shape Continuous Light for Multispectral Detection." Appl. Sci. 6, no. 8: 229.
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