Folate Functionalized PLGA Nanoparticles Loaded with Plasmid pVAX1-NH36: Mathematical Analysis of Release
AbstractPlasmid DNA (pVAX1-NH36) was encapsulated in nanoparticles of poly-dl-lactic-co-glycolic (PLGA) functionalized with polyethylene glycol (PEG) and folic acid (PLGA-PEG-FA) without losing integrity. PLGA-PEG-FA nanoparticles loaded with pVAX1-NH36 (pDNA-NPs) were prepared by using a double emulsification-solvent evaporation technique. PLGA-PEG-FA synthesis was verified by FT-IR and spectrophotometry methods. pVAX1-NH36 was replicated in Escherichia coli (E. coli) cell cultures. Atomic force microscopy (AFM) analysis confirmed pDNA-NPs size with an average diameter of 177–229 nm, depending on pVAX1-NH36 loading and zeta potentials were below −24 mV for all preparations. In vitro release studies confirmed a multiphase release profile for the duration of more than 30-days. Plasmid release kinetics were analyzed with a release model that considered simultaneous contributions of initial burst and degradation-relaxation of nanoparticles. Fitting of release model against experimental data presented excellent correlation. This mathematical analysis presents a novel approach to describe and predict the release of plasmid DNA from biodegradable nanoparticles. View Full-Text
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Gutiérrez-Valenzuela, C.A.; Guerrero-Germán, P.; Tejeda-Mansir, A.; Esquivel, R.; Guzmán-Z, R.; Lucero-Acuña, A. Folate Functionalized PLGA Nanoparticles Loaded with Plasmid pVAX1-NH36: Mathematical Analysis of Release. Appl. Sci. 2016, 6, 364.
Gutiérrez-Valenzuela CA, Guerrero-Germán P, Tejeda-Mansir A, Esquivel R, Guzmán-Z R, Lucero-Acuña A. Folate Functionalized PLGA Nanoparticles Loaded with Plasmid pVAX1-NH36: Mathematical Analysis of Release. Applied Sciences. 2016; 6(12):364.Chicago/Turabian Style
Gutiérrez-Valenzuela, Cindy A.; Guerrero-Germán, Patricia; Tejeda-Mansir, Armando; Esquivel, Reynaldo; Guzmán-Z, Roberto; Lucero-Acuña, Armando. 2016. "Folate Functionalized PLGA Nanoparticles Loaded with Plasmid pVAX1-NH36: Mathematical Analysis of Release." Appl. Sci. 6, no. 12: 364.
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