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Appl. Sci. 2016, 6(12), 364; doi:10.3390/app6120364

Folate Functionalized PLGA Nanoparticles Loaded with Plasmid pVAX1-NH36: Mathematical Analysis of Release

1
Nanotechnology Graduate Program, Department of Physics, University of Sonora, Hermosillo 83000, Mexico
2
Department of Chemical and Metallurgical Engineering, University of Sonora, Hermosillo 83000, Mexico
3
Department of Scientific and Technological Research, University of Sonora, Hermosillo 83000, Mexico
4
Department of Chemical and Environmental Engineering, University of Arizona, Tucson, AZ 85721, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Jordi Puiggalí
Received: 15 October 2016 / Revised: 9 November 2016 / Accepted: 11 November 2016 / Published: 25 November 2016
(This article belongs to the Special Issue Biodegradable and Biocompatible Nanoparticles)
View Full-Text   |   Download PDF [1670 KB, uploaded 25 November 2016]   |  

Abstract

Plasmid DNA (pVAX1-NH36) was encapsulated in nanoparticles of poly-dl-lactic-co-glycolic (PLGA) functionalized with polyethylene glycol (PEG) and folic acid (PLGA-PEG-FA) without losing integrity. PLGA-PEG-FA nanoparticles loaded with pVAX1-NH36 (pDNA-NPs) were prepared by using a double emulsification-solvent evaporation technique. PLGA-PEG-FA synthesis was verified by FT-IR and spectrophotometry methods. pVAX1-NH36 was replicated in Escherichia coli (E. coli) cell cultures. Atomic force microscopy (AFM) analysis confirmed pDNA-NPs size with an average diameter of 177–229 nm, depending on pVAX1-NH36 loading and zeta potentials were below −24 mV for all preparations. In vitro release studies confirmed a multiphase release profile for the duration of more than 30-days. Plasmid release kinetics were analyzed with a release model that considered simultaneous contributions of initial burst and degradation-relaxation of nanoparticles. Fitting of release model against experimental data presented excellent correlation. This mathematical analysis presents a novel approach to describe and predict the release of plasmid DNA from biodegradable nanoparticles. View Full-Text
Keywords: drug delivery; pVAX1-NH36; mathematical analysis; copolymer synthesis; leishmaniasis; folate nanoparticles drug delivery; pVAX1-NH36; mathematical analysis; copolymer synthesis; leishmaniasis; folate nanoparticles
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Gutiérrez-Valenzuela, C.A.; Guerrero-Germán, P.; Tejeda-Mansir, A.; Esquivel, R.; Guzmán-Z, R.; Lucero-Acuña, A. Folate Functionalized PLGA Nanoparticles Loaded with Plasmid pVAX1-NH36: Mathematical Analysis of Release. Appl. Sci. 2016, 6, 364.

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