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Appl. Sci. 2016, 6(11), 314; doi:10.3390/app6110314

The Development of Piezo-Driven Tools for Cellular Piercing

1,2,* , 1
and
3,4
1
Jiangsu Provincial Key Laboratory of Advanced Robotics, Soochow University, Suzhou 215021, China
2
Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215021, China
3
Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China
4
Jiangsu Province Key Laboratory of Stem Cell Research, Soochow University, Suzhou 215006, China
*
Author to whom correspondence should be addressed.
Academic Editor: Kohji Masuda
Received: 21 August 2016 / Revised: 26 September 2016 / Accepted: 17 October 2016 / Published: 26 October 2016
(This article belongs to the Special Issue Biomedical Ultrasound)
View Full-Text   |   Download PDF [2822 KB, uploaded 26 October 2016]   |  

Abstract

Conventionally, intracytoplasmic sperm injection (ICSI) in the mouse is conducted with piezo-drills that use a droplet of mercury for damping. The use of mercury causes concerns of toxicity and contamination. Although Fluorinert can be used as a substitute for mercury to reduce piezo-drill’s lateral vibration, the damping effect is not as satisfactory as mercury. In this work, a modified piezo-drill without using mercury was developed for the cellular piercing of mouse oocytes. Experimentally, appropriate parameters of driving voltage and frequency were obtained for the penetration of the zona pellucida of mouse oocytes. Furthermore, the lateral vibration of the injection pipette is lower than 1 μm in deionized water, which is not observable at 400 magnificence. With the piezo-drill without using mercury, the system performs the cellular piercing of mouse oocytes with a maximum cleavage rate of 94.7% (n = 117). View Full-Text
Keywords: modified piezo-drill; piezoelectric actuation; lateral vibration; mouse oocyte piercing modified piezo-drill; piezoelectric actuation; lateral vibration; mouse oocyte piercing
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Ru, C.; Pan, P.; Chen, R. The Development of Piezo-Driven Tools for Cellular Piercing. Appl. Sci. 2016, 6, 314.

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