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Appl. Sci. 2016, 6(10), 279; doi:10.3390/app6100279

Image Processing and Cell Phone Microscopy to Analyze the Immunomagnetic Beads on Micro-Contact Printed Gratings

1
BioMINDS (Bio Micro/Nano Devices and Sensors) Laboratory, Department of Electrical and Electronics Engineering, Abdullah Gül University, Kayseri 38080, Turkey
2
Bioengineering Department, Abdullah Gül University, Kayseri 38080, Turkey
Academic Editor: Samuel B. Adeloju
Received: 27 August 2016 / Revised: 20 September 2016 / Accepted: 21 September 2016 / Published: 28 September 2016
(This article belongs to the Section Optics and Lasers)
View Full-Text   |   Download PDF [3402 KB, uploaded 28 September 2016]   |  

Abstract

In this paper we report an ultra-low-cost spherical ball lens based cell phone microscopy and image processing algorithms to analyze the amount of immunomagnetic beads on micro-contact printed gratings. The spherical ball lens provides approximately 100× magnification but the recorded images are not clear and are noisy. By using the image-processing algorithms, the noise can be reduced and the images can be enhanced to quantify the amount of immunomagnetic beads on micro-contact printed lines. This method, which is portable and low-cost, can be an alternative read out mechanism for biosensing applications using immunomagnetic beads on micro-contact printed surface receptors. Further, 0.0335 mg/mL was the lowest magnetic bead concentration that could be detected above the inherent noise level of the spherical ball lens. View Full-Text
Keywords: cell phone microscopy; micro-contact printing; immunomagnetic beads; image processing; spherical ball lens cell phone microscopy; micro-contact printing; immunomagnetic beads; image processing; spherical ball lens
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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İçöz, K. Image Processing and Cell Phone Microscopy to Analyze the Immunomagnetic Beads on Micro-Contact Printed Gratings. Appl. Sci. 2016, 6, 279.

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