Next Article in Journal
Strategies for the Simulation of Sea Ice Organic Chemistry: Arctic Tests and Development
Next Article in Special Issue
About the Possibility of Disposal of HLRW in Deep Boreholes in Germany
Previous Article in Journal
Influence of Substratum Hydrophobicity on the Geomicrobiology of River Biofilm Architecture and Ecology Analyzed by CMEIAS Bioimage Informatics
Previous Article in Special Issue
Response of Compacted Bentonites to Thermal and Thermo-Hydraulic Loadings at High Temperatures
Article Menu
Issue 3 (September) cover image

Export Article

Open AccessArticle
Geosciences 2017, 7(3), 57; doi:10.3390/geosciences7030057

The Impact of Biofilms upon Surfaces Relevant to an Intermediate Level Radioactive Waste Geological Disposal Facility under Simulated Near-Field Conditions

1
Department of Biological Sciences, School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield HD1 3DH, UK
2
Department of Chemical Sciences, School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield HD1 3DH, UK
3
Bio-Imaging Facility, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK
*
Author to whom correspondence should be addressed.
Received: 27 April 2017 / Revised: 4 July 2017 / Accepted: 7 July 2017 / Published: 12 July 2017
View Full-Text   |   Download PDF [2996 KB, uploaded 12 July 2017]   |  

Abstract

The ability of biofilms to form on a range of materials (cementious backfill (Nirex Reference Vault Backfill (NRVB)), graphite, and stainless steel) relevant to potential UK intermediate level radioactive waste (ILW) disposal concepts was investigated by exposing these surfaces to alkaliphilic flocs generated by mature biofilm communities. Flocs are aggregates of biofilm material that are able to act as a transport vector for the propagation of biofilms. In systems where biofilm formation was observed there was also a decrease in the sorption of isosaccharinic acids to the NRVB. The biofilms were composed of cells, extracellular DNA (eDNA), proteins, and lipids with a smaller polysaccharide fraction, which was biased towards mannopyranosyl linked carbohydrates. The same trend was seen with the graphite and stainless steel surfaces at these pH values, but in this case the biofilms associated with the stainless steel surfaces had a distinct eDNA basal layer that anchored the biofilm to the surface. At pH 13, no structured biofilm was observed, rather all the surfaces accumulated an indistinct organic layer composed of biofilm materials. This was particularly the case for the stainless steel coupons which accumulated relatively large quantities of eDNA. The results demonstrate that there is the potential for biofilm formation in an ILW-GDF provided an initiation source for the microbial biofilm is present. They also suggest that even when conditions are too harsh for biofilm formation, exposed surfaces may accumulate organic material such as eDNA. View Full-Text
Keywords: NRVB; intermediate level waste; alkaliphilic; biofilm; engineered barrier; graphite; stainless steel; carbonation; isosaccharinic acid; eDNA NRVB; intermediate level waste; alkaliphilic; biofilm; engineered barrier; graphite; stainless steel; carbonation; isosaccharinic acid; eDNA
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Charles, C.J.; Rout, S.P.; Laws, A.P.; Jackson, B.R.; Boxall, S.A.; Humphreys, P.N. The Impact of Biofilms upon Surfaces Relevant to an Intermediate Level Radioactive Waste Geological Disposal Facility under Simulated Near-Field Conditions. Geosciences 2017, 7, 57.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Geosciences EISSN 2076-3263 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top