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Microorganisms 2018, 6(3), 67; https://doi.org/10.3390/microorganisms6030067

Transcription of IVIAT and Virulence Genes in Photobacterium damselae subsp. piscicida Infecting Solea senegalensis

1
Departamento de Microbiología, Universidad de Málaga, Andalucia Tech, Campus de Teatinos s/n, 29071 Málaga, Spain
2
Fish Immunology and Vaccinology Group, IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, 4200-135 Porto, Portugal
3
i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal
4
IFAPA Centro El Toruño, Camino Tiro Pichón s/n, 11500 El Puerto de Santa María (Cádiz), Spain
*
Authors to whom correspondence should be addressed.
Received: 19 June 2018 / Revised: 9 July 2018 / Accepted: 10 July 2018 / Published: 12 July 2018
(This article belongs to the Special Issue Marine Vibrios and Photobacteria: Taxonomy, Ecology and Pathogenesis)
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Abstract

Photobacterium damselae subsp. piscicida (Phdp) is responsible for disease outbreaks in marine aquaculture worldwide. Solea senegalensis, a valuable fish species for aquaculture in the south of Europe, is frequently affected by this pathogen. It is well established that bacteria respond to environmental signals and, in the case of pathogens, this ability may determine the outcome of their interaction with the host. Determination of gene expression under in vivo conditions constitutes a valuable tool in the assessment of microbial pathogenesis. Considering that different hosts may represent different environments for the pathogen, expression of Phdp virulence and in vivo induced antigen (IVIAT) genes during S. senegalensis infection has been determined in the present work. Increased transcription of genes encoding proteins involved in iron acquisition (Irp1, Irp2, HutB and HutD), oxidative stress defence (AhpC and Sod), adhesion (PDP_0080), toxins (AIP56) and metabolism (Impdh, Shmt and AlaRS) were detected in Phdp infecting S. senegalensis head kidney or liver. The highest increases corresponded to genes involved in survival under iron limiting conditions and oxidative stress, indicating their essential role during infection of sole. Results obtained give insight into Phdp virulence strategies and contribute to the identification of promising targets for the control of photobacteriosis. View Full-Text
Keywords: Photobacterium damselae subsp. piscicida; virulence; gene expression; in vivo; Solea senegalensis Photobacterium damselae subsp. piscicida; virulence; gene expression; in vivo; Solea senegalensis
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Núñez-Díaz, J.A.; Fumanal, M.; do Vale, A.; Fernández-Díaz, C.; Moriñigo, M.Á.; Balebona, M.C. Transcription of IVIAT and Virulence Genes in Photobacterium damselae subsp. piscicida Infecting Solea senegalensis. Microorganisms 2018, 6, 67.

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