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Microorganisms 2018, 6(1), 5; https://doi.org/10.3390/microorganisms6010005

Enzymatic Preparation of 2,5-Furandicarboxylic Acid (FDCA)—A Substitute of Terephthalic Acid—By the Joined Action of Three Fungal Enzymes

1
Department of Bio- and Environmental Sciences, Technische Universität Dresden—International Institute Zittau, 02763 Zittau, Germany
2
Natural and Environmental Sciences, University of Applied Sciences Zittau/Görlitz, 02763 Zittau, Germany
*
Author to whom correspondence should be addressed.
Received: 6 December 2017 / Revised: 5 January 2018 / Accepted: 6 January 2018 / Published: 9 January 2018
(This article belongs to the Special Issue Filamentous Fungi in White Biotechnology)
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Abstract

Enzymatic oxidation of 5-hydroxymethylfurfural (HMF) and its oxidized derivatives was studied using three fungal enzymes: wild-type aryl alcohol oxidase (AAO) from three fungal species, wild-type peroxygenase from Agrocybe aegerita (AaeUPO), and recombinant galactose oxidase (GAO). The effect of pH on different reaction steps was evaluated and apparent kinetic data (Michaelis-Menten constants, turnover numbers, specific constants) were calculated for different enzyme-substrate ratios and enzyme combinations. Finally, the target product, 2,5-furandicarboxylic acid (FDCA), was prepared in a multi-enzyme cascade reaction combining three fungal oxidoreductases at micro-scale. Furthermore, an oxidase-like reaction is proposed for heme-containing peroxidases, such as UPO, horseradish peroxidase, or catalase, causing the conversion of 5-formyl-2-furancarboxylic acid into FDCA in the absence of exogenous hydrogen peroxide. View Full-Text
Keywords: HMF; hydroxymethylfurfural; UPO; unspecific peroxygenase; aryl alcohol oxidase; galactose oxidase; Agrocybe aegerita; Pleurotus ostreatus; Pleurotus eryngii; Bjerkandera adusta HMF; hydroxymethylfurfural; UPO; unspecific peroxygenase; aryl alcohol oxidase; galactose oxidase; Agrocybe aegerita; Pleurotus ostreatus; Pleurotus eryngii; Bjerkandera adusta
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Karich, A.; Kleeberg, S.B.; Ullrich, R.; Hofrichter, M. Enzymatic Preparation of 2,5-Furandicarboxylic Acid (FDCA)—A Substitute of Terephthalic Acid—By the Joined Action of Three Fungal Enzymes. Microorganisms 2018, 6, 5.

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