Next Article in Journal
Combating Fusarium Infection Using Bacillus-Based Antimicrobials
Previous Article in Journal
Physiological Peculiarities of Lignin-Modifying Enzyme Production by the White-Rot Basidiomycete Coriolopsis gallica Strain BCC 142
Article Menu

Export Article

Open AccessArticle
Microorganisms 2017, 5(4), 74; doi:10.3390/microorganisms5040074

Pan-Cellulosomics of Mesophilic Clostridia: Variations on a Theme

1
Department of Biomolecular Sciences, The Weizmann Institute of Science, Rehovot 7610001, Israel
2
Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv 6997801, Israel
3
Architecture et Fonction des Macromolecules Biologiques, CNRS and Universite Aix-Marseilles I & II, Marseilles 13288, France
4
Faculty of Natural Sciences, Ben-Gurion University of the Negev, Beer-Sheva 8499000, Israel
*
Authors to whom correspondence should be addressed.
Received: 13 October 2017 / Revised: 14 November 2017 / Accepted: 16 November 2017 / Published: 18 November 2017
(This article belongs to the Section Environmental Microbiology)
View Full-Text   |   Download PDF [5103 KB, uploaded 20 November 2017]   |  

Abstract

The bacterial cellulosome is an extracellular, multi-enzyme machinery, which efficiently depolymerizes plant biomass by degrading plant cell wall polysaccharides. Several cellulolytic bacteria have evolved various elaborate modular architectures of active cellulosomes. We present here a genome-wide analysis of a dozen mesophilic clostridia species, including both well-studied and yet-undescribed cellulosome-producing bacteria. We first report here, the presence of cellulosomal elements, thus expanding our knowledge regarding the prevalence of the cellulosomal paradigm in nature. We explored the genomic organization of key cellulosome components by comparing the cellulosomal gene clusters in each bacterial species, and the conserved sequence features of the specific cellulosomal modules (cohesins and dockerins), on the background of their phylogenetic relationship. Additionally, we performed comparative analyses of the species-specific repertoire of carbohydrate-degrading enzymes for each of the clostridial species, and classified each cellulosomal enzyme into a specific CAZy family, thus indicating their putative enzymatic activity (e.g., cellulases, hemicellulases, and pectinases). Our work provides, for this large group of bacteria, a broad overview of the blueprints of their multi-component cellulosomal complexes. The high similarity of their scaffoldin clusters and dockerin-based recognition residues suggests a common ancestor, and/or extensive horizontal gene transfer, and potential cross-species recognition. In addition, the sporadic spatial organization of the numerous dockerin-containing genes in several of the genomes, suggests the importance of the cellulosome paradigm in the given bacterial species. The information gained in this work may be utilized directly or developed further by genetically engineering and optimizing designer cellulosome systems for enhanced biotechnological biomass deconstruction and biofuel production. View Full-Text
Keywords: cellulosomes; cohesin; dockerin; scaffoldin; glycoside hydrolases cellulosomes; cohesin; dockerin; scaffoldin; glycoside hydrolases
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Dassa, B.; Borovok, I.; Lombard, V.; Henrissat, B.; Lamed, R.; Bayer, E.A.; Moraïs, S. Pan-Cellulosomics of Mesophilic Clostridia: Variations on a Theme. Microorganisms 2017, 5, 74.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Microorganisms EISSN 2076-2607 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top