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Microorganisms 2017, 5(4), 67; doi:10.3390/microorganisms5040067

A Two-Step Bioconversion Process for Canolol Production from Rapeseed Meal Combining an Aspergillus niger Feruloyl Esterase and the Fungus Neolentinus lepideus

1
INRA Institut National de la Recherche Agronomique, Aix Marseille Univ., UMR1163 BBF Biodiversité et Biotechnologie Fongiques, 163 Avenue de Luminy, 13288 Marseille CEDEX 09, France
2
Terres Inovia, Parc Industriel, 11 Rue Monge, 33600 Pessac, France
3
Centre International de Ressources Microbiennes, Champignons Filamenteux, CIRM-CF, Case 925, 163 Avenue de Luminy, 13288 Marseille CEDEX 09, France
4
CIRAD Centre de coopération Internationale en Recherche Agronomique pour le Développement, UMR IATE Montpellier SupAgro-INRA, 2, Place Pierre Viala, 34060 Montpellier, France
5
Terres Univia, 11 rue Monceau, CS60003, 75378 Paris CEDEX 8, France
6
Sécurité et Qualité des Produits d’Origine Végétale, INRA Institut National de la Recherche Agronomique UMR408 SQPOV, Université d’Avignon, 33 rue Louis Pasteur, 84029 Avignon, France
*
Author to whom correspondence should be addressed.
Received: 5 September 2017 / Revised: 2 October 2017 / Accepted: 11 October 2017 / Published: 14 October 2017
(This article belongs to the Special Issue Filamentous Fungi in White Biotechnology)
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Abstract

Rapeseed meal is a cheap and abundant raw material, particularly rich in phenolic compounds of biotechnological interest. In this study, we developed a two-step bioconversion process of naturally occurring sinapic acid (4-hydroxy-3,5-dimethoxycinnamic acid) from rapeseed meal into canolol by combining the complementary potentialities of two filamentous fungi, the micromycete Aspergillus niger and the basidiomycete Neolentinus lepideus. Canolol could display numerous industrial applications because of its high antioxidant, antimutagenic and anticarcinogenic properties. In the first step of the process, the use of the enzyme feruloyl esterase type-A (named AnFaeA) produced with the recombinant strain A. niger BRFM451 made it possible to release free sinapic acid from the raw meal by hydrolysing the conjugated forms of sinapic acid in the meal (mainly sinapine and glucopyranosyl sinapate). An amount of 39 nkat AnFaeA per gram of raw meal, at 55 °C and pH 5, led to the recovery of 6.6 to 7.4 mg of free sinapic acid per gram raw meal, which corresponded to a global hydrolysis yield of 68 to 76% and a 100% hydrolysis of sinapine. Then, the XAD2 adsorbent (a styrene and divinylbenzene copolymer resin), used at pH 4, enabled the efficient recovery of the released sinapic acid, and its concentration after elution with ethanol. In the second step, 3-day-old submerged cultures of the strain N. lepideus BRFM15 were supplied with the recovered sinapic acid as the substrate of bioconversion into canolol by a non-oxidative decarboxylation pathway. Canolol production reached 1.3 g/L with a molar yield of bioconversion of 80% and a productivity of 100 mg/L day. The same XAD2 resin, when used at pH 7, allowed the recovery and purification of canolol from the culture broth of N. lepideus. The two-step process used mild conditions compatible with green chemistry. View Full-Text
Keywords: Aspergillus niger; canolol; feruloyl esterase; Neolentinus lepideus; rapeseed meal; sinapic acid Aspergillus niger; canolol; feruloyl esterase; Neolentinus lepideus; rapeseed meal; sinapic acid
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MDPI and ACS Style

Odinot, E.; Fine, F.; Sigoillot, J.-C.; Navarro, D.; Laguna, O.; Bisotto, A.; Peyronnet, C.; Ginies, C.; Lecomte, J.; Faulds, C.B.; Lomascolo, A. A Two-Step Bioconversion Process for Canolol Production from Rapeseed Meal Combining an Aspergillus niger Feruloyl Esterase and the Fungus Neolentinus lepideus. Microorganisms 2017, 5, 67.

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