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Microorganisms 2017, 5(3), 57; doi:10.3390/microorganisms5030057

The Skin Bacterium Propionibacterium acnes Employs Two Variants of Hyaluronate Lyase with Distinct Properties

1
Department of Biomedicine, Aarhus University, 8000 Aarhus C, Denmark
2
Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark
*
Author to whom correspondence should be addressed.
Received: 2 August 2017 / Revised: 7 September 2017 / Accepted: 8 September 2017 / Published: 12 September 2017
(This article belongs to the Special Issue The Functions of the Microbiome in Skin Health and Disease)
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Abstract

Hyaluronic acid (HA) and other glycosaminoglycans are extracellular matrix components in the human epidermis and dermis. One of the most prevalent skin microorganisms, Propionibacterium acnes, possesses HA-degrading activity, possibly conferred by the enzyme hyaluronate lyase (HYL). In this study, we identified the HYL of P. acnes and investigated the genotypic and phenotypic characteristics. Investigations include the generation of a P. acnes hyl knockout mutant and HYL activity assays to determine the substrate range and formed products. We found that P. acnes employs two distinct variants of HYL. One variant, HYL-IB/II, is highly active, resulting in complete HA degradation; it is present in strains of the phylotypes IB and II. The other variant, HYL-IA, has low activity, resulting in incomplete HA degradation; it is present in type IA strains. Our findings could explain some of the observed differences between P. acnes phylotype IA and IB/II strains. Whereas type IA strains are primarily found on the skin surface and associated with acne vulgaris, type IB/II strains are more often associated with soft and deep tissue infections, which would require elaborate tissue invasion strategies, possibly accomplished by a highly active HYL-IB/II. View Full-Text
Keywords: Propionibacterium acnes; hyaluronate lyase; hyaluronic acid; glycosaminoglycan; extracellular matrix; skin microbiota Propionibacterium acnes; hyaluronate lyase; hyaluronic acid; glycosaminoglycan; extracellular matrix; skin microbiota
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Nazipi, S.; Stødkilde, K.; Scavenius, C.; Brüggemann, H. The Skin Bacterium Propionibacterium acnes Employs Two Variants of Hyaluronate Lyase with Distinct Properties. Microorganisms 2017, 5, 57.

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