Next Article in Journal
Achromobacter Species Isolated from Cystic Fibrosis Patients Reveal Distinctly Different Biofilm Morphotypes
Previous Article in Journal
Correia Repeat Enclosed Elements and Non-Coding RNAs in the Neisseria Species
Previous Article in Special Issue
Enrichment of Fusobacteria in Sea Surface Oil Slicks from the Deepwater Horizon Oil Spill
Article Menu

Export Article

Open AccessArticle
Microorganisms 2016, 4(3), 32; doi:10.3390/microorganisms4030032

Phylogenetic Characterization of Marine Benthic Archaea in Organic-Poor Sediments of the Eastern Equatorial Pacific Ocean (ODP Site 1225)

1
Department of Marine Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
2
Biology Department, California State University Bakersfield, Bakersfield, CA 93311-1022, USA
3
Ramboll, Copenhagen DK-2300, Denmark
*
Author to whom correspondence should be addressed.
Academic Editors: Don Cowan and Johannes F. Imhoff
Received: 20 April 2016 / Revised: 16 July 2016 / Accepted: 22 August 2016 / Published: 6 September 2016
(This article belongs to the Special Issue Diversity and Dynamics of Marine Microbial Communities)
View Full-Text   |   Download PDF [1254 KB, uploaded 8 September 2016]   |  

Abstract

Sequencing surveys of microbial communities in marine subsurface sediments have focused on organic-rich, continental margins; the database for organic-lean deep-sea sediments from mid-ocean regions is underdeveloped. The archaeal community in subsurface sediments of ODP Site 1225 in the eastern equatorial Pacific (3760 m water depth; 1.1 and 7.8 m sediment depth) was analyzed by PCR, cloning and sequencing, and by denaturant gradient gel electrophoresis (DGGE) of 16S rRNA genes. Three uncultured archaeal lineages with different depth distributions were found: Marine Group I (MG-I) within the Thaumarchaeota, its sister lineage Marine Benthic Group A (MBG-A), the phylum-level archaeal lineage Marine Benthic Group B (also known as Deep-Sea Archaeal Group or Lokiarchaeota), and the Deep-Sea Euryarchaeotal Group 3. The MG-I phylotypes included representatives of sediment clusters that are distinct from the pelagic members of this phylum. On the scale from fully oxidized, extremely organic carbon-depleted sediments (for example, those the South Pacific Gyre) to fully reduced, organic carbon-rich marine subsurface sediments (such as those of the Peru Margin), Ocean Drilling Program (ODP) Site 1225 falls into the non-extreme organic carbon-lean category, and harbors archaeal communities from both ends of the spectrum. View Full-Text
Keywords: Marine archaea; Marine Group I; subsurface; marine sediment; Ocean Drilling Program Marine archaea; Marine Group I; subsurface; marine sediment; Ocean Drilling Program
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Lauer, A.; Sørensen, K.B.; Teske, A. Phylogenetic Characterization of Marine Benthic Archaea in Organic-Poor Sediments of the Eastern Equatorial Pacific Ocean (ODP Site 1225). Microorganisms 2016, 4, 32.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Microorganisms EISSN 2076-2607 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top