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Microorganisms 2015, 3(4), 725-745; doi:10.3390/microorganisms3040725

Dynamic In Vitro Models of the Human Gastrointestinal Tract as Relevant Tools to Assess the Survival of Probiotic Strains and Their Interactions with Gut Microbiota

1
Accueil Conception, Ingénierie et Développement de l’Aliment et du Médicament (EA 4678 CIDAM), Centre de Recherche en Nutrition Humaine Auvergne, Université d’Auvergne, Centre Biomédical de Recherche et de Valorisation (CBRV) 28 place Henri Dunant, 63000 Clermont-Ferrand, France
2
Microbes, Intestin, Inflammation et Susceptibilité de l’Hôte (M2iSH), Unité Mixte de Recherche Institut National de la Santé Et de la Recherche Médicale (UMR INSERM) / Université d’Auvergne U1071 Unité Sous Contrat - Institut National de Recherche Agronomique (USC-INRA) 2018, Centre de Recherche en Nutrition Humaine Auvergne, Université d’Auvergne, CBRV 28 place Henri Dunant, 63000 Clermont-Ferrand, France
3
Service de Bactériologie, Centre Hospitalier Universitaire (CHU), Clermont-Ferrand, 58 rue Montalembert, 63000 Clermont-Ferrand, France
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Carl Gordon Johnston
Received: 8 June 2015 / Revised: 8 June 2015 / Accepted: 11 September 2015 / Published: 23 October 2015
(This article belongs to the Special Issue Host-Gut Microbiota Metabolic Interactions)
View Full-Text   |   Download PDF [8137 KB, uploaded 23 October 2015]   |  

Abstract

The beneficial effects of probiotics are conditioned by their survival during passage through the human gastrointestinal tract and their ability to favorably influence gut microbiota. The main objective of this study was to use dynamic in vitro models of the human digestive tract to investigate the effect of fasted or fed state on the survival kinetics of the new probiotic Saccharomyces cerevisiae strain CNCM I-3856 and to assess its influence on intestinal microbiota composition and activity. The probiotic yeast showed a high survival rate in the upper gastrointestinal tract whatever the route of admistration, i.e., within a glass of water or a Western-type meal. S. cerevisiae CNCM I-3856 was more sensitive to colonic conditions, as the strain was not able to colonize within the bioreactor despite a twice daily administration. The main bacterial populations of the gut microbiota, as well as the production of short chain fatty acids were not influenced by the probiotic treatment. However, the effect of the probiotic on the gut microbiota was found to be individual dependent. This study shows that dynamic in vitro models can be advantageously used to provide useful insight into the behavior of probiotic strains in the human digestive environment. View Full-Text
Keywords: probiotic; Saccharomyces cerevisiae; survival; in vitro models; human gastrointestinal tract; intestinal microbiota; food matrix probiotic; Saccharomyces cerevisiae; survival; in vitro models; human gastrointestinal tract; intestinal microbiota; food matrix
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Cordonnier, C.; Thévenot, J.; Etienne-Mesmin, L.; Denis, S.; Alric, M.; Livrelli, V.; Blanquet-Diot, S. Dynamic In Vitro Models of the Human Gastrointestinal Tract as Relevant Tools to Assess the Survival of Probiotic Strains and Their Interactions with Gut Microbiota. Microorganisms 2015, 3, 725-745.

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