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Microorganisms 2015, 3(3), 327-343; doi:10.3390/microorganisms3030327

Tetrachloromethane-Degrading Bacterial Enrichment Cultures and Isolates from a Contaminated Aquifer

1
CNRS Génétique Moléculaire, Génomique, Microbiologie, Université de Strasbourg, UMR 7156 UNISTRA-CNRS, 28 rue Goethe, 67083 Strasbourg, France
2
Environmental Research and Innovation (ERIN), Luxembourg Institute of Science and Technology (LIST), 5 avenue des Hauts-Fourneaux, 4362 Esch/Alzette, Luxembourg
*
Author to whom correspondence should be addressed.
Academic Editors: Marina G. Kalyuzhnaya and Ludmila Chistoserdova
Received: 29 April 2015 / Revised: 9 June 2015 / Accepted: 18 June 2015 / Published: 2 July 2015
(This article belongs to the Special Issue Microbial C1 Metabolism)
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Abstract

The prokaryotic community of a groundwater aquifer exposed to high concentrations of tetrachloromethane (CCl4) for more than three decades was followed by terminal restriction fragment length polymorphism (T-RFLP) during pump-and-treat remediation at the contamination source. Bacterial enrichments and isolates were obtained under selective anoxic conditions, and degraded 10 mg·L−1 CCl4, with less than 10% transient formation of chloroform. Dichloromethane and chloromethane were not detected. Several tetrachloromethane-degrading strains were isolated from these enrichments, including bacteria from the Klebsiella and Clostridium genera closely related to previously described CCl4 degrading bacteria, and strain TM1, assigned to the genus Pelosinus, for which this property was not yet described. Pelosinus sp. TM1, an oxygen-tolerant, Gram-positive bacterium with strictly anaerobic metabolism, excreted a thermostable metabolite into the culture medium that allowed extracellular CCl4 transformation. As estimated by T-RFLP, phylotypes of CCl4-degrading enrichment cultures represented less than 7%, and archaeal and Pelosinus strains less than 0.5% of the total prokaryotic groundwater community. View Full-Text
Keywords: C1 compound biodegradation; tetrachloromethane; carbon tetrachloride; bacterial communities; bacterial enrichment cultures; co-metabolism; chlorinated solvents; Pelosinus sp. TM1 C1 compound biodegradation; tetrachloromethane; carbon tetrachloride; bacterial communities; bacterial enrichment cultures; co-metabolism; chlorinated solvents; Pelosinus sp. TM1
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Penny, C.; Gruffaz, C.; Nadalig, T.; Cauchie, H.-M.; Vuilleumier, S.; Bringel, F. Tetrachloromethane-Degrading Bacterial Enrichment Cultures and Isolates from a Contaminated Aquifer. Microorganisms 2015, 3, 327-343.

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