Whole Genome Classification and Phylogenetic Analyses of Rotavirus B strains from the United States
AbstractRotaviruses (RVs) are a major etiological agent of acute viral gastroenteritis in humans and young animals, with rotavirus B (RVB) often detected in suckling and weaned pigs. Group A rotavirus classification is currently based on the two outer capsid proteins, VP7 and VP4, and the middle layer protein, VP6. Using RVB strains generated in this study and reference sequences from GenBank, pairwise identity frequency graphs and phylogenetic trees were constructed for the eleven gene segments of RVB to estimate the nucleotide identity cutoff values for different genotypes and determine the genotype diversity per gene segment. Phylogenetic analysis of VP7, VP4, VP6, VP1–VP3, and NSP1–NSP5 identified 26G, 5P, 13I, 5R, 5C, 5M, 8A, 10N, 6T, 4E, and 7H genotypes, respectively. The analysis supports the previously proposed cutoff values for the VP7, VP6, NSP1, and NSP3 gene segments (80%, 81%, 76% and 78%, respectively) and suggests new cutoff values for the VP4, VP1, VP2, VP3, NSP2, NSP4, and NSP5 (80%, 78%, 79%, 77% 83%, 76%, and 79%, respectively). Reassortment events were detected between the porcine RVB strains from our study. This research describes the genome constellations for the complete genome of Group B rotaviruses in different host species. View Full-Text
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Shepherd, F.K.; Herrera-Ibata, D.M.; Porter, E.; Homwong, N.; Hesse, R.; Bai, J.; Marthaler, D.G. Whole Genome Classification and Phylogenetic Analyses of Rotavirus B strains from the United States. Pathogens 2018, 7, 44.
Shepherd FK, Herrera-Ibata DM, Porter E, Homwong N, Hesse R, Bai J, Marthaler DG. Whole Genome Classification and Phylogenetic Analyses of Rotavirus B strains from the United States. Pathogens. 2018; 7(2):44.Chicago/Turabian Style
Shepherd, Frances K.; Herrera-Ibata, Diana M.; Porter, Elizabeth; Homwong, Nitipong; Hesse, Richard; Bai, Jianfa; Marthaler, Douglas G. 2018. "Whole Genome Classification and Phylogenetic Analyses of Rotavirus B strains from the United States." Pathogens 7, no. 2: 44.
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