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Pathogens 2018, 7(2), 37; https://doi.org/10.3390/pathogens7020037

Identification and In Silico Characterization of a Genetically Distinct Avian Rotavirus D Capsid Gene, VP7

1
Division of Biological Standardization, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, Uttar Pradesh, India
2
Department of Veterinary Pathology, G.B. Pant University of Agriculture and Technology, Pantnagar 263145, Uttarakhand, India
3
Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, Uttar Pradesh, India
*
Author to whom correspondence should be addressed.
Received: 11 March 2018 / Revised: 31 March 2018 / Accepted: 3 April 2018 / Published: 4 April 2018
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Abstract

Rotavirus D (RV-D) is gaining importance as a cause of gastroenteritis and runting and stunting syndrome (RSS) in poultry. To date, information is scarce on the molecular analysis of RV-D isolates worldwide. In this study, the VP7 gene, a major constituent of outer capsid structural protein, from a RV-D isolate (UKD48) obtained from Uttarakhand state was analyzed. Phylogenetically, the RV-D isolate was found to be closely related to a South Korean strain, and the nucleotide percent identity varied from 80.4–84.2% with other RV-D strains available globally. Furthermore, domain investigation within 21 aligned amino acid sequences of the VP7 gene affirmed that this gene has several domains: a conserved glycosylation site (N–I–T) having an important role in protein folding; a N-terminal signal peptide (“ITG”) for endoplasmic reticulum retention; and two hydrophobic sites for elucidating transmembrane portions, antigenic structures, and so forth. The findings suggest that the VP7 gene of the Indian RV-D isolate is genetically distinct from those of other avian RV-Ds. Although biological evidence is still needed to prove the functional characteristics of these domains in outer capsid structural proteins, the present study adds new knowledge and derives the need for further investigation. View Full-Text
Keywords: avian rotaviruses; rotavirus D; VP7 gene; phylogenetic analysis; domain identification avian rotaviruses; rotavirus D; VP7 gene; phylogenetic analysis; domain identification
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Deol, P.; Kattoor, J.J.; Sircar, S.; Batra, M.; Dhama, K.; Malik, Y.S. Identification and In Silico Characterization of a Genetically Distinct Avian Rotavirus D Capsid Gene, VP7. Pathogens 2018, 7, 37.

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