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Pathogens 2016, 5(3), 46; doi:10.3390/pathogens5030046

Simultaneous Quantification and Differentiation of Streptococcus suis Serotypes 2 and 9 by Quantitative Real-Time PCR, Evaluated in Tonsillar and Nasal Samples of Pigs

1
Faculty of Veterinary Medicine, Department of Farm Animal Health, Utrecht University, P.O. Box 80.151, 3508 TD Utrecht, The Netherlands
2
Faculty of Veterinary Medicine, Department of Infectious Diseases and Immunology, Utrecht University, P.O. Box 80.165, 3508 TD Utrecht, The Netherlands
3
Central Veterinary Institute of Wageningen UR, P.O. Box 65, 8200 AB Lelystad, The Netherlands
*
Author to whom correspondence should be addressed.
Academic Editor: Mariela Segura
Received: 15 April 2016 / Revised: 23 June 2016 / Accepted: 27 June 2016 / Published: 30 June 2016
(This article belongs to the Special Issue Streptococcus suis)
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Abstract

Invasive Streptococcus suis (S. suis) infections in pigs are often associated with serotypes 2 and 9. Mucosal sites of healthy pigs can be colonized with these serotypes, often multiple serotypes per pig. To unravel the contribution of these serotypes in pathogenesis and epidemiology, simultaneous quantification of serotypes is needed. A quantitative real-time PCR (qPCR) targeting cps2J (serotypes 2 and 1/2) and cps9H (serotype 9) was evaluated with nasal and tonsillar samples from S. suis exposed pigs. qPCR specifically detected serotypes in all pig samples. The serotypes loads in pig samples estimated by qPCR showed, except for serotype 9 in tonsillar samples (correlation coefficient = 0.25), moderate to strong correlation with loads detected by culture (correlation coefficient > 0.65), and also in pigs exposed to both serotypes (correlation coefficient > 0.75). This qPCR is suitable for simultaneous differentiation and quantification of important S. suis serotypes. View Full-Text
Keywords: Streptococcus suis; S. suis; serotype 2; serotype 9; cps; qPCR; pig; tonsil; nose Streptococcus suis; S. suis; serotype 2; serotype 9; cps; qPCR; pig; tonsil; nose
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MDPI and ACS Style

Dekker, N.; Daemen, I.; Verstappen, K.; de Greeff, A.; Smith, H.; Duim, B. Simultaneous Quantification and Differentiation of Streptococcus suis Serotypes 2 and 9 by Quantitative Real-Time PCR, Evaluated in Tonsillar and Nasal Samples of Pigs. Pathogens 2016, 5, 46.

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