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Pathogens 2016, 5(2), 35; doi:10.3390/pathogens5020035

Combination of Heat Shock and Enhanced Thermal Regime to Control the Growth of a Persistent Legionella pneumophila Strain

1
Department of Civil Engineering, Polytechnique Montréal, Montréal, QC H3T 1J4, Canada
2
CIUSSSE—Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, QC J1G 2E8, Canada
3
Department of Microbiology and Infectious Diseases, Université de Sherbrooke, QC J1K 2R1, Canada
*
Author to whom correspondence should be addressed.
Academic Editor: Lawrence S. Young
Received: 15 March 2016 / Revised: 4 April 2016 / Accepted: 11 April 2016 / Published: 15 April 2016
(This article belongs to the Special Issue Pathogen Legionella pneumophila)
View Full-Text   |   Download PDF [1532 KB, uploaded 15 April 2016]   |  

Abstract

Following nosocomial cases of Legionella pneumophila, the investigation of a hot water system revealed that 81.5% of sampled taps were positive for L. pneumophila, despite the presence of protective levels of copper in the water. A significant reduction of L. pneumophila counts was observed by culture after heat shock disinfection. The following corrective measures were implemented to control L. pneumophila: increasing the hot water temperature (55 to 60 °C), flushing taps weekly with hot water, removing excess lengths of piping and maintaining a water temperature of 55 °C throughout the system. A gradual reduction in L. pneumophila counts was observed using the culture method and qPCR in the 18 months after implementation of the corrective measures. However, low level contamination was retained in areas with hydraulic deficiencies, highlighting the importance of maintaining a good thermal regime at all points within the system to control the population of L. pneumophila. View Full-Text
Keywords: legionellosis; sanitary hot water; temperature diagnostic; Legionella pneumophila; culture; quantitative polymerase chain reaction; copper concentration; hospital premise plumbing legionellosis; sanitary hot water; temperature diagnostic; Legionella pneumophila; culture; quantitative polymerase chain reaction; copper concentration; hospital premise plumbing
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Bédard, E.; Boppe, I.; Kouamé, S.; Martin, P.; Pinsonneault, L.; Valiquette, L.; Racine, J.; Prévost, M. Combination of Heat Shock and Enhanced Thermal Regime to Control the Growth of a Persistent Legionella pneumophila Strain. Pathogens 2016, 5, 35.

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