Pathogens 2016, 5(1), 3; doi:10.3390/pathogens5010003
Brighter Fluorescent Derivatives of UTI89 Utilizing a Monomeric vGFP
1
Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, 1E Kent Ridge Road, NUHS Tower Block, Level 10, Singapore 119074, Singapore
2
Infectious Diseases Group, Genome Institute of Singapore, 60 Biopolis street, Genome, #02-01, Singapore 138672, Singapore
*
Author to whom correspondence should be addressed.
Academic Editor: Catharina Svanborg
Received: 11 September 2015 / Revised: 2 December 2015 / Accepted: 29 December 2015 / Published: 5 January 2016
(This article belongs to the Special Issue Molecular Aspects of Urinary Tract Infection)
Abstract
Fluorescent proteins, especially green fluorescent protein (GFP), have been instrumental in understanding urinary tract infection pathogenesis by uropathogenic Escherichia coli (UPEC). We have used a recently developed GFP variant, vsfGFP-9, to create new plasmid- and chromosome-based GFP derivatives of the UPEC strain UTI89. The vsfGFP-9 strains are nearly 10× brighter with no in vitro growth or in vivo virulence defects compared to previously reported GFP-expressing UTI89 strains. The chromosomal vsfGFP-9 strain is equivalent to the wild type UTI89 during in vivo UTI, while both plasmid GFP constructs have an equivalent virulence defect compared to non-plasmid carrying UTI89. These new vsfGFP-9 expressing strains should be useful for further studies of the pathogenesis of UTI89, and similar strategies can be used to create improved fluorescent derivatives of other UPEC strains. View Full-TextKeywords:
GFP; uropathogenic Escherichia coli; urinary tract infection
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Eshaghi, M.; Mehershahi, K.S.; Chen, S.L. Brighter Fluorescent Derivatives of UTI89 Utilizing a Monomeric vGFP. Pathogens 2016, 5, 3.
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