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Pathogens 2015, 4(2), 269-282; doi:10.3390/pathogens4020269

Impact of Environmental Factors on Legionella Populations in Drinking Water

1
School of Life Sciences, Arizona State University, Tempe, AZ 85281, USA
2
School of Sustainable Engineering and the Built Environment, Arizona State University, Tempe, AZ 85281, USA
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Mark W. LeChevallier
Received: 9 April 2015 / Revised: 12 May 2015 / Accepted: 13 May 2015 / Published: 19 May 2015
(This article belongs to the Special Issue Waterborne Pathogens)
View Full-Text   |   Download PDF [313 KB, uploaded 20 May 2015]   |  

Abstract

To examine the impact of environmental factors on Legionella in drinking water distribution systems, the growth and survival of Legionella under various conditions was studied. When incubated in tap water at 4 °C, 25 °C, and 32 °C, L. pneumophila survival trends varied amongst the temperatures, with the stable populations maintained for months at 25 °C and 32 °C demonstrating that survival is possible at these temperatures for extended periods in oligotrophic conditions. After inoculating coupons of PVC, copper, brass, and cast iron, L. pneumophila colonized biofilms formed on each within days to a similar extent, with the exception of cast iron, which contained 1-log less Legionella after 90 days. L. pneumophila spiked in a model drinking water distribution system colonized the system within days. Chlorination of the system had a greater effect on biofilm-associated Legionella concentrations, with populations returning to pre-chlorination levels within six weeks. Biofilms sampled from drinking water meters collected from two areas within central Arizona were analyzed via PCR for the presence of Legionella. Occurrence in only one area indicates that environmental differences in water distribution systems may have an impact on the survival of Legionella. These results document the impact of different environmental conditions on the survival of Legionella in water. View Full-Text
Keywords: Legionella pneumophila; survival; drinking water; distribution system; temperature; biofilm Legionella pneumophila; survival; drinking water; distribution system; temperature; biofilm
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Schwake, D.O.; Alum, A.; Abbaszadegan, M. Impact of Environmental Factors on Legionella Populations in Drinking Water. Pathogens 2015, 4, 269-282.

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